Abstract
Mesenchymal stem cells (MSCs) are pluripotent progenitors and can differentiate into a variety of cell types, including osteoblasts, chondrocytes, and adipocytes. Vascular endothelial growth factor (VEGF) has been shown to stimulate endothelial cell mitogenesis and migrations. To investigate whether VEGF could induce rat bone marrow-derived MSCs differentiation into endothelial cells, we isolated and cultured rat bone marrow-derived MSCs from the femur of the Sprague–Dawley rats. MSCs were treated with VEGF for 2, 4, 7 days. The cellular morphology changes of MSCs were observed under phase contrast microscope. Then we tested the expression of endothelial cell markers VEGF receptor fms-related tyrosine kinase 1 (Flt-1) by reverse-transcription PCR (RT-PCR), western blot, and immunofluorescence technology. Our results showed that the expression levels of Flt-1 were significantly increased in time-dependent manner. The above results show that VEGF can induce mesenchymal stem cells differentiation into endothelial cells. The data provides the basis of constructing tissue engineering vascular.
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Acknowledgments
This work was financially supported by National Natural Science Foundation of China (31171303), Program for Changjiang Scholars and Innovative Research Team in University of Ministry of Education of China (IRT1166) and the Key Project of Chinese Ministry of Education (212010).
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Wang, N., Zhang, R., Wang, S., du, J., Tang, Y., Zhang, TC. (2014). Vascular Endothelial Growth Factor Induces Bone Marrow-Derived Mesenchymal Stem Cells Differentiation into Endothelial Cells. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_167
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DOI: https://doi.org/10.1007/978-3-642-37925-3_167
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