Abstract
The random DNA fragments (500–3000 bp), which obtained from genomic of avian Pasteuella multocida CVCC474 with restriction enzyme Sau3AI were cloned into the BamHIsite of the eukaryotic expression vector pcDNA3.1(+) to construct the genomic expression library. The library was subdivided into five clone pools and the recombinant plasmids were extracted from each group. The Balb/c mice named groups 1–5 were vaccinated with these recombinant plasmids respectively, and the mice injected with pcDNA3.1(+) and PBS were negative control. After immunization the serum antibodies were tested by ELISA. Immunized mice were challenged with virulent of avian Pasteurella multocida, the relative protection rate was counted. The results showed the levels of antibodies and the relative protection rate in group one was significantly higher than 1–4 groups (P < 0.05) and control groups (P < 0.01). The results indicated genomic expression library vaccines provide a promising approach for the prevention of avian pasteurellosis.
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Acknowledgments
This work was supported by a grant from the Natural scientific research Project of Henan province, China (No. 2011B180019).
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Gong, Q., Qin, C., Cheng, M. (2014). Construction of Genomic Expression Library Vaccines Against Avian Pasteurella Multocida and Detection of the Immune Efficacy. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_139
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DOI: https://doi.org/10.1007/978-3-642-37925-3_139
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