Abstract
The strain F17 which had broad antibacterial spectrum and strong antibacterial activity was isolated from Pinctada fucata sample collected from Sanya, China. This strain was finally identified as Bacillus flexus based on morphological, biochemical characteristics, and 16S rRNA sequence. Antifungal spectrum was confirmed by oxford piate assay system and showed that F17 had significant inhibitory effect against Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Listeria monocytogenes, Pseudomoas aerunginosa, Salmonella typhimurium, Vibrio parahaemolyticus, Shigella dysenteriae, etc. The volatilis components of the extracellular metabolites of F17 were analyzed by GC–MS. The results showed that the main chemical compositions were 2-Methyl butyric acid, Dioctyl phthalate, 2,3-Butanediol, 4-Chloro-2-nitrobenzyl alcohol, and Glycyl-l-proline in the fermentation fluid extracted by ethyl acetate. In those, 2-Methyl butyric acid and 2,3-Butanediol can be used in food flavor; 4-Chloro-2-nitrobenzyl alcohol has certain development potential for antiseptic. Glycyl-l-proline is an important raw material in pharmaceutical and nutrient food industry. The experiments showed the metabolites of F17 had great potential as food additives.
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Acknowledgments
This work was financially supported by National Science and Technology Planning Project (2012BAD28B05), Hainan Key Science and Technology Project (ZDXM20100005), Guangdong province Science and Technology Project (2011B031200009).
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Wu, Y., Zhang, Y., Li, L., Yang, X., Shi, W., Shao, Z. (2014). Identification of Symbiotic or Epiphyte Microbe F17 from Pinctada Fucata and Analysis of Its Main Metabolites by GC–MS. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 251. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37925-3_138
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DOI: https://doi.org/10.1007/978-3-642-37925-3_138
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