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RNA Interference and Applications in Plants

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 250))

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Abstract

RNA interference (RNAi) inhibits gene expression in a very specific way. It has a vital significance in the regulation of gene expression, the defense of viral infection, the controlling of jumping genes. In recent years, RNAi has become an important method of studying gene function with its high specificity, efficiency, hereditability and other significant advantages and has been used in the study of functional genomics and traits improvement. This paper simply introduces the RNAi mechanism and discusses the applications of RNAi in plant functional genomics, crop quality improvement, and resistance of disease and insect.

Fund project: project supporting by science and technology in Tianjin, sweet sorghum polymer molecular breeding (10ZCKFNC00100).

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References

  1. Guo S, Kemphues KJ (1995) Par-1, a gene required for establishing polarity in C. elegans embryos, encodes a putative Ser/Thr kinase that is asymmetrically distributed. Cell 81(4):611–620

    Article  CAS  Google Scholar 

  2. Fire A, Xu S, Montgomery MK (1998) Potent and specific genetic interference by double stranded RNA in Caenorhabditis elegans. Nature 391:806–811

    Article  CAS  Google Scholar 

  3. Hamilton AJ, Baulcombe DC (1999) A species of small antisense RNA in post-transcriptional gene silencing in plants. Science 286:950–952

    Article  CAS  Google Scholar 

  4. Bernstein E, Caudy A, Hammond SM, Hannon GJ (2001) Role for a bidentate ribonuclease in the initiation step of RNA interference. Nature 409:363–366

    Article  CAS  Google Scholar 

  5. Kim VN (2003) RNA interference in functional genomics and medicine. Korean Med. Sci. 18:309–318

    Article  CAS  Google Scholar 

  6. Chuang C, Meyerowitz EM (2000) Specific and heritable genetic interference by double stranded RNA in Arabidopsis thaliana. Proc Natl Acad Sci USA 97(9):4985–4990

    Article  CAS  Google Scholar 

  7. Padmanaban S, Lin XY, Perera I (2004) Differential expression of vacuolar H+ 2ATPase subunit c genes in tissues active in membrane trafficking and their roles in plant growth as revealed by RNAi. Plant Physiol 134:113

    Article  Google Scholar 

  8. Moritoh S, Miki D, Akiyama M (2005) RNAi mediated silencing of OsGEN-L (OsGEN-like), a new member of the RAD-XPG nuclease family, causes male sterility by defect of microspore development in rice. Plant Cell Physiol 46:699–715

    Article  CAS  Google Scholar 

  9. Travella S, Klimm TE, Keller B (2006) RNA interference-based gene silencing as an efficient tool for functional genomics in hexaploid bread wheat. Plant Physiol 142:6–20

    Article  CAS  Google Scholar 

  10. Li Q, Tao G, Qiu Y (2009) RNAi vector construction of potato Y virus gene CP and determination of interference effect. Genom Appl Biol 28(3):460–464

    Google Scholar 

  11. Zibu W, Qi J (2011) Construction of the RNAi vector and expression of starch branch enzyme gene in wheat grain endosperm, mol. Plant Breed 9(4):438–442

    Google Scholar 

  12. Liu Q, Singh SP, Green AG (2002) High-stearic and high-oleic Cotton seed oils produced by hairpin RNA-mediated post-transcriptional gene silencing. Plant Physiol 129(4):1732–1743

    Article  CAS  Google Scholar 

  13. Ogita S, Uefuji H, Yube Y (2003) Producing decaffeinated coffee plants. Nature 423(19):823

    Article  CAS  Google Scholar 

  14. Tang G, Galili G (2004) Using RNAi to improve plant nutritional value: from mechanism to application. Trends Biotechnol 22(9):463–469

    Article  CAS  Google Scholar 

  15. Fukusaki E, Kawasaki K, Kajiyama S et al (2004) Flower color modulations of Torenia hybrida by down regulation of chalcone sythase genes with RNA interference. J Biotechnol 111(3):229–240

    Article  CAS  Google Scholar 

  16. Chen Y, Li H (2007) The cloning of tomatos ACO gene and inhibition of its RNAi to release ethylene. J Agri Biotechnol 15(3):464–468

    CAS  Google Scholar 

  17. Wan Q, Zhang X, Song M (2007) RNAi specific mediated gene Lcy silenced to increase the content of lycopenein tomatoes. J Biol Eng 23(3):429–433

    CAS  Google Scholar 

  18. Lichtenstein C, Klee H, Montoya A (1984) Nucleotide sequence and transcript mapping of the gene of the pTiANC octopine Ti-plasmid: a bacterial gene involved in plant tumori genesis. J Mol Appl Genet 2:354–362

    CAS  Google Scholar 

  19. Ooms G, Hooykaas PJ, Moolenaar G (1981) Crown gall plant tumors of abnormal morphology, induced by Agro bacterium tumefaciens carrying mutated octopine Ti plasmids; analysis of T2 DNA functions. Gene 14:33–50

    Article  CAS  Google Scholar 

  20. Depicker A, van Montagu M, Schell J (1978) Homologous DNA sequences in different Tip lasmids are essential for oncogenicity. Nature 275:150–153

    Article  CAS  Google Scholar 

  21. Waterhouse PM, Graham MW, Wang MB (1998) Virus resistance and gene silencing in plants can be induced by simultaneous expression of sense and antisense RNA. Proc Natl Acad Sci USA 95(23):13959–13964

    Article  CAS  Google Scholar 

  22. Pinto YM, Kok RA, Baulcombe DC (1999) Resistance to rice yellow mottle virus (RYMV) in cultivated African rice varieties containing RYMV transgenes. Nat Biotechnol 17:702–707

    Article  CAS  Google Scholar 

  23. Wang MB, Abbott DC, Waterhouse PM (2000) A single copy of a virus-derived transgene encoding hairpin RNA gives immunity to barley yellow dwarf virus. Mol Plant Pathol 1(6):3472356

    Article  Google Scholar 

  24. Kalantidis K, Psaradakis S, Tabler M (2002) The occurrence of CMV2 specific short RNAs in transgenic tobacco expressing virus derived double-stranded RNA is indicative of resistance t o the virus. MPMI 15(8):8262833

    Article  Google Scholar 

  25. Tenlado F, Dlaz- Rufz JR (2001) Double- stranded RNA- mediated interference with plant virus infection. J Virol 75(24):12288–12297

    Article  Google Scholar 

  26. Andika IB, Kondo H, Tamada T (2005) Evidence that RNA silencing-mediated resistance to Beet necrotic yellow vein virus is less effective in roots than in leaves. MPMI 18(3):194–204

    Article  CAS  Google Scholar 

  27. Mao YB, Cai WJ, Wang JW (2007) Silencing a cotton boll worn P450 monooxygenase gene by plant-mediated RNAi impairs larval tolerance of gossypol. Nat Biotechnol 25:1307–1313

    Article  CAS  Google Scholar 

  28. Bautista M, Tanaka T (2009) RNA interference mediated knockdown of a cytochrome P450, CYP6BG1, from the diamondback moth, Plutella xylostella, reduces larval resistance to permethrin. Insect Biochem Mol Biol 39:38–46

    Article  CAS  Google Scholar 

  29. Cigan AM, Unger Wallace E, Collet H (2005) Source transcriptional gene silencing as a tool for uncovering gene function in maize. Plant J 43:929–940

    Article  CAS  Google Scholar 

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Author information

Authors and Affiliations

  1. Department of Agronomy, Tianjin Agricultural University, Tianjin, 300384, People’s Republic of China

    Yunrong An, Zhongyou Pei, Nan Xin & Haifeng Wang

Authors
  1. Yunrong An
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  2. Zhongyou Pei
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  3. Nan Xin
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  4. Haifeng Wang
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Corresponding author

Correspondence to Zhongyou Pei .

Editor information

Editors and Affiliations

  1. College of Bioengineering ,Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas- Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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An, Y., Pei, Z., Xin, N., Wang, H. (2014). RNA Interference and Applications in Plants. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 250. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37922-2_102

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  • DOI: https://doi.org/10.1007/978-3-642-37922-2_102

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37921-5

  • Online ISBN: 978-3-642-37922-2

  • eBook Packages: EngineeringEngineering (R0)

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