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The Application of Salting-Out in the Analysis of Methoxy-Phenolic Compounds in Pu-erh Tea

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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012)

Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

Abstract

This paper presents the application of salting-out in the analysis of methoxy-phenolic compounds in pu-erh tea by using headspace solid microextraction (HS-SPME) combined with gas chromatography mass spectrometry (GC/MS). At first, the influences of extraction and the best extraction condition were investigated, include SPME fibers, extraction time and extraction temperature. Under the optimum condition, the salting-out performances of NH4Cl, KCl, NaCl, MgCl2, Na2SO4, (NH4)2SO4 and C6H5O7Na3 were studied. Sodium citrate exhibits the highest extraction efficiency. Compared with unsalted, the total peak area of methoxy-phenolic compounds increased by 2.8 times with sodium citrate added. With C6H5O7Na3, the proposed method affords wide of linearity, good linear regression coefficients (0.992–0.999), detection limits varied from 1.54 to 14.53 ng/g and the relative standard deviations for repeatability were below 10 %. Recovery was ranged from 82.05 to 112.04 % and the relative standard deviations were below 10 %.

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Acknowledgments

This work was financially supported by the program of National High Technology Research and Development Program of China (863 Program) (Grant No. SS2012AA023408), the Cheung Kong Scholars, and Innovative Research Team Program in University of Ministry of Education, China (Grant No. IRT1166).

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Authors and Affiliations

  1. Key Laboratory of Industrial Fermentation Microbiology, Tianjin Industrial Microbiology Key Laboratory, Ministry of Education, College of Biotechnology, Tianjin University of Science & Technology, Tianjin, 300457, People’s Republic of China

    Chao Wang, Liping Du, Yan Lu, Tao Li, Jianxun Li, Wei Li & Dongguang Xiao

  2. Yunnan Tasly Biology Tea Technology Limited Incorporation, Yunnan, 665000, People’s Republic of China

    Changwen Li & Yongquan Xu

Authors
  1. Chao Wang
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  2. Liping Du
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  3. Yan Lu
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  4. Tao Li
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  5. Jianxun Li
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  6. Wei Li
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  7. Dongguang Xiao
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  8. Changwen Li
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  9. Yongquan Xu
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Corresponding author

Correspondence to Liping Du .

Editor information

Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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Wang, C. et al. (2014). The Application of Salting-Out in the Analysis of Methoxy-Phenolic Compounds in Pu-erh Tea. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_45

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_45

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

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