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Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012) pp 359–366Cite as

Breeding of Streptomyces diastatochromogenes for Mass-Producing ε-Poly-L-Lysine by Composite Mutation

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Part of the book series: Lecture Notes in Electrical Engineering ((LNEE,volume 249))

Abstract

In order to improve the productivity of ε-Poly-l-Lysine (ε-PL) by Streptomyces diastatochromogenes Cbγ4, the selection and breeding of mass-producing strains were carried out through the single spore suspension treated with several groups of the composite mutation of NTG and UV and LiCl. The result showed that a high yield producing strain 6#−7 was obtained through 0.8 g/L NTG for 45 min, followed with UV irradiation for 45 s under magnetic stirring, and then through a series of screening, such as resistant plate, methylene blue plate, shake-flask screening. With the good genetic stability, the yield of ε-PL could reach 0.775 ± 0.046 g/L at 72 h, 42.2 % higher than the initial strain. Moreover, the mutant displayed a great potential for increasing productivity in later period of fermentation.

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Acknowledgments

This work was supported by the National Natural Science Foundation (21276197), the National High-tech R&D Program (863 Program, 2013AA102106) and the National Science and Technology Program of China (2011BAD23B05-3).

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Authors and Affiliations

  1. Key Laboratory of Industrial Microbiology, Ministry of Education, College of Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, People’s Republic of China

    Shuai Song, Zhilei Tan, Fengzhu Guo, Xue Zhang, Qingchao Song & Shiru Jia

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  1. Shuai Song
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  2. Zhilei Tan
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  3. Fengzhu Guo
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  4. Xue Zhang
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  5. Qingchao Song
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  6. Shiru Jia
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Corresponding author

Correspondence to Shiru Jia .

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Editors and Affiliations

  1. College of Bioengineering, Tianjin University of Science and Technology, Tianjin, People's Republic of China

    Tong-Cun Zhang

  2. Nanjing University of Technology, Nanjing, People's Republic of China

    Pingkai Ouyang

  3. Department of Microbiology & Molecular Genetics, University of Texas Houston Medical School, Texas, Texas, USA

    Samuel Kaplan

  4. Wellcome Trust Sanger Institute, Cambridge, United Kingdom

    Bill Skarnes

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© 2014 Springer-Verlag Berlin Heidelberg

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Song, S., Tan, Z., Guo, F., Zhang, X., Song, Q., Jia, S. (2014). Breeding of Streptomyces diastatochromogenes for Mass-Producing ε-Poly-L-Lysine by Composite Mutation. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_37

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  • DOI: https://doi.org/10.1007/978-3-642-37916-1_37

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-37915-4

  • Online ISBN: 978-3-642-37916-1

  • eBook Packages: EngineeringEngineering (R0)

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