Abstract
Optimization of four process parameters was attempted using Box-Behnken design for production of diosgenin by a mixed culture with Aspergillus oryzae, Phanerochaete chrysosporium, and Aspergillus niger. Maximum diosgenin yield of 42.89 ± 0.53 mg/g was obtained after optimization of culture conditions such as Dioscorea zingiberensis C.H.Wright (DZW) concentration 42.98 g/l, inoculum size 1.73 ml of spore suspension (1: 2: 3 ratio of A. oryzae, P. chrysosporium, and A. niger), initial pH 5.0, and cultivation time 5.27 days (127 h) and incubated at 30 °C on a rotary shaker set at 180 r/min as mixed culture.
Keywords
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This work was financially supported by the Tianjin Science and Technology Support Plan Project (10ZCKFSH0060) and the Natural Science Foundation of China (No. 20906071).
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Xie, J., Xu, X., Bie, S. (2014). Optimization of Diosgenin Production by Mixed Culture Using Response Surface Methodology. In: Zhang, TC., Ouyang, P., Kaplan, S., Skarnes, B. (eds) Proceedings of the 2012 International Conference on Applied Biotechnology (ICAB 2012). Lecture Notes in Electrical Engineering, vol 249. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-37916-1_12
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DOI: https://doi.org/10.1007/978-3-642-37916-1_12
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