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Microfluidic Tools for DNA Analysis

Abstract

This chapter introduces the use of microfluidic tools for DNA analysis. It will cover both qualitative analysis and quantitative analysis. A microfluidic device typically implies multicomponent integration. Most reviews in scientific journals discuss microfluidics by a sequential introduction for each component. In order to present the great power of microfluidics as emerging tools for DNA research, we organized each section according to the primary function that can be achieved by a kind of microfluidic device, and emphasize the primary innovation leading to the unique function for each specific device. As microfluidic tools showed many distinct advantages over existing approaches and thus hold dramatic commercial potential, we will also discuss the problems during the commercialization process of microfluidic devices. DNA analysis we discussed herein includes amplification, detection, sequencing, counting, sizing, and weighing, and our perspective covers a wide range of related fields including chemistry, molecular biology, physics, and micro/nano-fabrication technologies, which reveals that DNA analysis on microfluidic devices is a highly interdisciplinary subject and will have lasting impact among biologists, chemists, physicians, and engineers.

Keywords

  • Microfluidic chip
  • DNA
  • Miniaturization
  • Amplification
  • Solid phase
  • Sample-in–answer-out
  • Droplet
  • Structural variation
  • Cantilever
  • Qualitative analysis
  • Quantitative analysis

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Zhang, Y., Jiang, X. (2013). Microfluidic Tools for DNA Analysis. In: Fan, C. (eds) DNA Nanotechnology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-36077-0_7

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