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Metal Remediation via In Vitro Root Cultures

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Plant-Based Remediation Processes

Part of the book series: Soil Biology ((SOILBIOL,volume 35))

Abstract

The in vitro root culture and hairy roots culture represent a relative new approach for metal removal studies. Both systems are characterized by indefinite growth and genetic and biochemical stability. Higher growth rates are obtained, and the analysis of metal removal can be performed under controlled conditions and without the participation of microbiota using these in vitro systems. This chapter reviews some examples of in vitro root cultures obtained from plants, such as Rubia tinctorum, Scirpus americanus, and Typha latifolia, that grow in contaminated sites, and their capacity to remove heavy metals from culture medium. The importance of genetically transformed roots with Agrobacterium tumefaciens in phytoremediation studies is presented. The capabilities of hairy roots to remove and accumulate Cd, Cu, Ni, U, and Zn and the mechanism of tolerance in response to these metals are included, too. Finally, brief information related to scale-up for the production of hairy roots using bioreactors and the perspective of this technology is analyzed.

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  1. Faculty of Chemistry, University of San Luis Potosí, Manuel Nava 6, CP 78210, San Luis Potosí, Mexico

    María del Socorro Santos-Díaz

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Correspondence to María del Socorro Santos-Díaz .

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  1. , Radiol. Impact Performance Div., Belgian Nuclear Research Centre, Boeretang 200, Mol, 2400, Belgium

    Dharmendra Kumar Gupta

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del Socorro Santos-Díaz, M. (2013). Metal Remediation via In Vitro Root Cultures. In: Gupta, D. (eds) Plant-Based Remediation Processes. Soil Biology, vol 35. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-35564-6_6

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