Update on Methods and Techniques to Study Endocytosis in Plants

  • Olga Šamajová
  • Tomáš Takáč
  • Daniel von Wangenheim
  • Ernst Stelzer
  • Jozef Šamaj
Chapter

Abstract

The growing interest in the investigation of endocytosis, vesicular transport routes, and corresponding regulatory mechanisms resulted in the exploitation of cell biological, genetic, biochemical, and proteomic approaches. Methods and techniques such as site-directed and T-DNA insertional mutagenesis, RNAi, classical inhibitor treatments, and recombinant GFP technology combined with confocal laser scanning microscopy (CLSM) and electron and immune-electron microscopy were routinely employed for investigation of endocytosis in plant cells. However, new approaches such as high-throughput confocal microscopy screens on mutants and proteomic analyses on isolated vesicular compartments and root cells treated with vesicular trafficking inhibitors (both focused on the identification of new endosomal proteins), together with chemical genomics and advanced microscopy approaches such as Förster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP), light sheet-based fluorescence microscopy, and super-resolution microscopy provided a significant amount of new data and these new methods appear as extremely promising tools in this field.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Olga Šamajová
    • 1
  • Tomáš Takáč
    • 1
  • Daniel von Wangenheim
    • 2
  • Ernst Stelzer
    • 2
  • Jozef Šamaj
    • 1
  1. 1.Centre of the Region Haná for Biotechnological and Agricultural Research, Faculty of Science, Department of Cell BiologyPalacký UniversityOlomoucCzech Republic
  2. 2.Physical Biology Group, Frankfurt Institute for Molecular Life Sciences (FMLS)Goethe Universität Frankfurt am MainFrankfurt am MainGermany

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