Cell Co-culture and Signaling Analysis Based on Microfluidic Devices Coupling with ESI-Q-TOF MS

  • Huibin Wei
Part of the Springer Theses book series (Springer Theses)


The cell analysis is carried out with the intention to explore the physiological activities of living organisms, therefore obtain useful information on of disease prevention and treatment. The living organism is an organic entity, and each organ exists interdependently. Every single external stimulation would cause a chain reaction of the related organs, which induce the whole body’s quick response. The signal transmission between organs essentially is the transmission of certain substance, which was secreted by the cells belonging to the stimulation-caused organ. This compound searches for the receptor on the target tissue, and further transfers the signal. Through regulating the content and type of the secretions acting as the signal factors, the information is transferred to the receptor cells, and response is made by the living organism. Therefore, cell signaling is a part of a complex system of communication that governs basic cellular activities and coordinates cell actions, which requires for further studies. The lack of perceiving and correctly responding to their microenvironment is one possible reason for the loss of functional capabilities of development, tissue repair, and immunity as well as normal tissue homeostasis, which causes diseases such as cancer, autoimmunity, and diabetes.


Growth Hormone PC12 Cell HepG2 Cell Microfluidic Device PDMS Layer 
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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Beijing Research Institute of Chemical Industry, SINOPECBeijingChina

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