Enhanced and Specific Internalization of Polymeric Nanoparticles to Cells
We designed new polymer nanoparticles embedding quantum dots with artificial cell membrane-surface as a highly sensitive bioimaging probe. These nanoparticles were prepared by assembling phospholipid polymer as a platform and oligopeptide as a bioaffinity moiety on the surface of the nanoparticles. They showed high resistance to non-specific cellular uptake from HeLa cells due to the nature of phospholipid polymer with phosphorylcholine groups. On the other hand, when arginine octapeptide was immobilized on the surface, they could permeate the membrane of HeLa cells effectively and good fluorescence based on quantum dots could be observed. Cytotoxicity was not produced by these nanoparticles even after immobilization of octapeptide. Thus we obtained stable fluorescent polymer nanoparticles covered with artificial cell membrane, which are useful as an excellent bioimaging probe evaluation for biomolecular function in the target cells.
KeywordsNanoparticles cellular internalization phospholipid polymers oligopeptide quantum dots
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