Study of Tetrodotoxin Detection in Cultured Medium

  • Quach T. Tu
  • Doan N. Hieu
  • Nguyen H. K. Tu
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 49)

Abstract

Detection of TTX produced in cultured medium containing peptone, yeast extract that was made from animal or other biological sources caused the false positive results when using thin layer chromatgraphy method. With such results, it was a lot of mistakes when detect tetrodotoxin in food as a toxin or in medicine as a drug. Therefore, it was needed to look up a modification in TTX detection in cultured medium. In the study, the tested samples were designed and the mobile phases for thin layer chromatgraphy were optimized. The results showed the suitable system for TTX detection was pyridine: ethylacetate: acetic acid: chloroform with the ratio as 15:5:5:2 or butanol: acetic acid: distilled water (15:3:6). The tetrodotoxin level was detectable at 10 ng.

Keywords

detection media thin layer chromatography tetrodotoxin 

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Copyright information

© IFMBE 2013

Authors and Affiliations

  • Quach T. Tu
    • 1
  • Doan N. Hieu
    • 1
  • Nguyen H. K. Tu
    • 1
  1. 1.School of BiotechnologyHochiminh City International UniversityHochiminh CityVietnam

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