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Detection of N-Acetyl-D-Glucosamine in Hyaluronan by Thin Layer Chromatography

  • Nguyen H. K. Tu
  • Pham V. M. Thien
Conference paper
Part of the IFMBE Proceedings book series (IFMBE, volume 49)

Abstract

Various reactions that produce color are known to be selective for certain types of carbohydrates. It was difficult to identify N-acetyl-glucosamine in hyaluronan because the hyaluronan was produced very low in microorgansims. Besides, in the traditional methods of Morgan-Elson, using alkali borate to lysis hyaluronan to give N-acety-D-glucosamine caused the unspecific products that could not react to di-methyl-benzaldehyde (DMAB). The study developed the method to detect N-acetyl-D-glucosamine by thin layer chromatography (TLC) that showed the stable purple traces on TLC than in liquid. The study was confirmed by applying high pressure liquid chromatography (HPLC).

Keywords

hyaluronan N-acetyl-d-glucosamine thin layer chromatography 

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Copyright information

© IFMBE 2013

Authors and Affiliations

  1. 1.School of BiotechnologyHochiminh City International UniversityHochiminh CityVietnam

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