Detection of Platelet Activation in Blood Tubing Line by Observing Ultra-Weak Luminescence
We have applied the luciferin-luciferase reaction and are developing a technique to detect the released ATP from platelets in their aggregation. A photomultiplier tube (PMT) was used as a photodetector and a serial counting device was connected to count the output signal from the PMT. Using this apparatus, the luminous reaction of rat heparinized blood was observed in two types of blood circuits; one was totally composed of poly vinyl chloride (PVC) tube and the other was inserted a glass tube section into the PVC circuit. As a result, after the removal of residual ATPs in plasma by luciferin, emission intensity progressively increased in the both circuits. In the circuit without a glass section, the emission intensity was remarkably lower.
Keywordsplatelet thrombus formation luciferin in vitro
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