Transepidermal Water Loss (TEWL)

Chapter

Abstract

The epidermal barrier is the outermost part of our body, or more precisely, of our skin. Approximately 90 % of the barrier function can be attributed to the stratum corneum. The importance of the epidermal barrier and more specifically the stratum corneum with its major components the corneocytes, the intercorneal bilamellar lipids, and the cornified envelope has become increasingly evident over the last years.

Skin as a barrier prevents the organism from loss of essential components such as ions, water, and serum proteins. The skin barrier also reflects internal processes, diseases, disease activity, and some of the lifestyle, manifested in intrinsic and extrinsic aging. It also has sociocultural functions and plays an important role in communication and self-expression. Transepidermal water loss (TEWL) is one of the most important parameters to evaluate the epidermal permeability barrier function of the skin. A low TEWL is generally a characteristic feature of an intact skin protective function. There are different methods for TEWL measurement, the unventilated (closed) chamber method, the ventilated chamber method, and the method using an open chamber. This chapter is mainly focused on the latter. To perform accurate and reliable measurements, variable factors related to the panelists, environment, and the instrument should be taken into account and will be addressed in this chapter.

Keywords

Atopic Dermatitis Stratum Corneum Skin Surface Skin Barrier Epidermal Barrier 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

SC

Stratum corneum

TEWL

Transepidermal water loss

References

  1. 1.
    Elias PM, Choi EH (2005) Interactions among stratum corneum defensive functions. Exp Dermatol 14(10):719–726PubMedCrossRefGoogle Scholar
  2. 2.
    Darlenski R, Sassning S, Tsankov N, Fluhr JW (2009) Non-invasive in vivo methods for investigation of the skin barrier physical properties. Eur J Pharm Biopharm 72(2):295–303PubMedCrossRefGoogle Scholar
  3. 3.
    Pinnagoda J, Tupker RA, Agner T, Serup J (1990) Guidelines for transepidermal water loss (TEWL) measurement. A report from the Standardization Group of the European Society of Contact Dermatitis. Contact Dermatitis 22(3):164–178PubMedCrossRefGoogle Scholar
  4. 4.
    Rogiers V (2001) EEMCO guidance for the assessment of transepidermal water loss in cosmetic sciences. Skin Pharmacol Appl Skin Physiol 14(2):117–128PubMedGoogle Scholar
  5. 5.
    Angelova-Fischer I, Bauer A, Hipler UC et al (2005) The objective severity assessment of atopic dermatitis (OSAAD) score: validity, reliability and sensitivity in adult patients with atopic dermatitis. Br J Dermatol 153(4):767–773PubMedCrossRefGoogle Scholar
  6. 6.
    Sugarman JL, Fluhr JW, Fowler AJ, Bruckner T, Diepgen TL, Williams ML (2003) The objective severity assessment of atopic dermatitis score: an objective measure using permeability barrier function and stratum corneum hydration with computer-assisted estimates for extent of disease. Arch Dermatol 139(11):1417–1422PubMedCrossRefGoogle Scholar
  7. 7.
    Miteva M, Richter S, Elsner P, Fluhr JW (2006) Approaches for optimizing the calibration standard of Tewameter TM 300. Exp Dermatol 15(11):904–912PubMedCrossRefGoogle Scholar
  8. 8.
    Fluhr JW, Feingold KR, Elias PM (2006) Transepidermal water loss reflects permeability barrier status: validation in human and rodent in vivo and ex vivo models. Exp Dermatol 15(7):483–492PubMedCrossRefGoogle Scholar
  9. 9.
    Fluhr JW (2011) Practical aspects of cosmetic testing. How to set up a scientific study in skin physiology. Springer, Berlin/HeidelbergGoogle Scholar

Copyright information

© Springer Berlin Heidelberg 2014

Authors and Affiliations

  1. 1.Department of DermatologyCharité – Universitätsmedizin BerlinBerlinGermany
  2. 2.Department of Dermatology and VenereologyTokuda HospitalSofiaBulgaria

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