Stratum Corneum Quantification
The stratum corneum (SC) is the outermost skin layer. It can be removed sequentially by repeated application of appropriate adhesive tapes commonly known as “SC tape stripping.” This technique is a relatively noninvasive method to investigate the structure, properties, and functions of SC as well as the penetration of topically applied substances ex vivo and in vivo. Other techniques to remove SC include skin surface biopsy using cyanoacrylate strips and diverse skin scraping techniques. SC represents the main barrier for skin penetration of xenobiotics. It consists of corneocytes embedded in lipid layers with a thickness between 5 and 20 μm in healthy adults, except in the SC of the palm and sole where SC is thicker. Since SC is known to be a reservoir for topically applied chemicals, its removal by tape stripping has provided useful data on their penetration into skin. Tape stripping is therefore regarded as a valuable method to evaluate cutaneous bioavailability and bioequivalence of topically applied substances. This chapter reviews the experimental procedure of tape-stripping technique and methods to quantify SC removal.
KeywordsStratum Corneum Tape Strip Simple Colorimetric Method Stratum Corneum Protein Molar Hydrochloric Acid
- 2.Surber C, Schwarb FP, Smith EW (1999) Tape-stripping technique. In: Bronaugh RL, Maibach H (eds) Percutaneous absorption: drugs – cosmetics – mechanisms – methodology, vol 97, 3rd edn, Drugs and the pharmaceutical sciences. Dekker, New York, pp 395–409Google Scholar
- 11.Weigmann HJ, Lademann J, Meffert H, Schaefer H, Sterry W (1999) Determination of the horny layer profile by tape stripping in combination with optical spectroscopy in the visible range as a prerequisite to quantify percutaneous absorption. Skin Pharmacol Appl Skin Physiol 12:34–45PubMedGoogle Scholar