Abstract
In this chapter, we present a methodology of documenting the angular anisotropy of skin elasticity with high sensitivity and dynamic range using the Reviscometer® RVM 600 and its use to document changes in keratinocyte morphology elicited by topical application of a reversible antagonist to keratinocyte nicotinic acetylcholine receptors (nAChRs). The method is based on determining the directional dependence of the speed of an acoustic shear wave on the skin surface at intervals of 3°. Based on the angular distribution of the resonance running time, we define two parameters: the anisotropy and the width of the angular dispersion. The mechanical properties of the skin are not isotropic (uniform in all directions), and there is a need to assess this angular anisotropy. During development, from infancy to adolescence, skin is expected to respond to tension isotropically to accommodate for growth, while in adulthood this isotropic behavior regresses due to site-specific habituation to tension. We find that with increasing age the anisotropy increases while the angular dispersion width decreases. Changes in viable epidermis keratinocyte morphology and stratum corneum moisturization may be assessed and documented by these parameters. The ratio of these values provides a sensitive parameter for the assessment of the directional behavior of the skin mechanical properties. This parameter provides a large effective dynamic range capable of demonstrating close to an order of magnitude difference in skin viscoelasticity from infants up to 75 years of age and documenting efficacy of topical treatments. Furthermore, we show that the direction of the angular anisotropy relates to the direction of the dermal cleavage lines as defined by Langer, indicating that the anisotropy of the mechanical properties of skin stems from structural parameters. Based on these results, we conclude that the proposed methodology is able to capture accurately age-related and morphological changes in the skin of the mechanical properties and demonstrate a structure-function relationship.
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Abbreviations
- ASTM:
-
American Society for Testing and Material Standards
- DMAE:
-
2-(Dimethylamino)ethanol
- FWHM:
-
Full width at half maximum
- IRHD:
-
International rubber hardness degree
- RRT:
-
Resonance running time
References
Elias PM (2005) Stratum corneum defensive functions: an integrated view. J Invest Dermatol 125:183–200
Lanir Y (1986) Skin mechanics. In: Skalak R, Chien S (eds) Handbook of bioengineering. McGraw-Hill, New York, pp 11.11–11.24
Nizet JL, Pierard-Franchimont C, Pierard GE (2001) Influence of body posture and gravitational forces on shear wave propagation in the skin. Dermatology 202:177–180
Langer K (1861) Zur anatomie und Physiologie der Haut: I. Uber die Splatbarkeit der Cutis. Sitzungber Akad Wiss Wien 44:19–46
Cox H (1942) The cleavage lines of the skin. Br J Surg 29:234–240
Gibson T, Stark H, Evans J (1969) Directional variation in extensibility of human skin in vivo. J Biomech 2:201–204
Takakuda K, Miyairi H (1996) Tensile behaviour of fibroblasts cultured in collagen gel. Biomaterials 17:1393–1397
Jansen L, Rottier PB (1957) Elasticity of human skin related to age. Dermatologica 115:106–111
Lavker RM, Zheng PS, Dong G (1987) Aged skin: a study by light, transmission electron, and scanning electron microscopy. J Invest Dermatol 88:44s–51s
Elsner P (1995) Skin elasticity. In: Bardesca E, Elsner P, Wilhelm K, Maibach H (eds) Bioengineering of the skin: methods and instrumentation. CRC Press, Boca Raton
Rodrigues L (2001) EEMCO guidance to the in vivo assessment of tensile functional properties of the skin. Part 2: instrumentation and test modes. Skin Pharmacol Appl Skin Physiol 14:52–67
Serup J, Jemec G, Grove G (2006) Handbook of non-invasive methods and the skin. CRC Press, Boca Raton
Dahlgren R, Elsnau W (1986) Measurement of skin condition by sonic velocity. J Soc Cosmet Chem 35:1–9
Davis BR, Bahniuk E, Young JK, Barnard CM, Mansour JM (1989) Age-dependent changes in the shear wave propagation through human skin. Exp Gerontol 24:201–210
Hermanns-Le T, Jonlet F, Scheen A, Pierard GE (2001) Age- and body mass index-related changes in cutaneous shear wave velocity. Exp Gerontol 36:363–372
Pereira JM, Mansour JM, Davis BR (1990) Analysis of shear wave propagation in skin; application to an experimental procedure. J Biomech 23:745–751
Vexler A, Polyansky I, Gorodetsky R (1999) Evaluation of skin viscoelasticity and anisotropy by measurement of speed of shear wave propagation with viscoelasticity skin analyzer. J Invest Dermatol 113:732–739
Varani J, Nickoloff BJ, Dixit VM, Mitra RS, Voorhees JJ (1989) All-trans retinoic acid stimulates growth of adult human keratinocytes cultured in growth factor-deficient medium, inhibits production of thrombospondin and fibronectin, and reduces adhesion. J Invest Dermatol 93:449–454
Berardesca E, Gabba P, Farinelle N, Borroni G, Rabbiosi G (1990) In vivo tretinoin-induced changes in skin mechanical properties. Br J Dermatol 122(4):525–529
Grando SA, Horton RM, Pereira EF, Diethelm-Okita BM, George PM, Albuquerque EX (1995) A nicotinic acetylcholine receptor regulating cell adhesion and motility is expressed in human Keratinocytes. J Invest Dermatol 105:774–781
Ruvolo E, Southall M, Bordoloi B, Lukenbach E, Lukenbach G (2009) Compositions for the treatment of signs of aging. US issued patent US20090292027 A1
Tronnier H (1980) Dermatologisch-phamakologische Methoden zur Prüfung kosmeticher Präparate und Grundstoffe. Ärzliche Kosmotogie 10:361–367
Kinsler LE, Frey AR, Coppens AB, Sanders JV (1999) Fundamentals of acoustics, 4th edn. John Wiley-Sons, Inc., New York
Potts RO, Chrisman DA, Burns EM (1983) The dynamic mechanical properties of human skin in vivo. J Biomech 16:365–372
Dorogi PL, DeWitt GM, Stone BR, Buras EM Jr (1986) Viscoelastometry of skin in vivo using shear wave propagation. Bioeng Skin 2:59–70
Mridha M, Odman S, Oberg PA (1992) Mechanical pulse wave propagation in gel, normal and oedematous tissues. J Biomech 25:1213–1218
Biscoe B, Sebastian K (1993) Analysis of the “durometer” indentation. Rubber Chem Technol 66:827–836
ASTM (2003) D2240-03 Standard test method for rubber property—durometer hardness. ASTM International, West Conshohocken
Ruvolo EC Jr, Stamatas GN, Kollias N (2007) Skin viscoelasticity displays site- and age-dependent angular anisotropy. Skin Pharmacol Physiol 20:313–321
Rajadhyaksha M, González S, Zavislan JM, Anderson R, Webb RH (1999) In vivo confocal scanning laser microscopy of human skin II: advances in instrumentation and comparison with histology. J Invest Dermatol 113:293–303
Uhoda I, Faska N, Robert C, Cauwenbergh G, Pierard GE (2002) Split face study on the cutaneous tensile effect of 2-dimethylaminoethanol (deanol) gel. Skin Research and Technology 8:164–167
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Ruvolo, E., Kollias, N. (2014). Assessment of Mechanical Properties of Skin by Shearwave Propagation and Acoustic Dispersion. In: Berardesca, E., Maibach, H., Wilhelm, KP. (eds) Non Invasive Diagnostic Techniques in Clinical Dermatology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32109-2_21
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DOI: https://doi.org/10.1007/978-3-642-32109-2_21
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