Application of Laser Scanning Microscopy in Dermatology and Cutaneous Physiology

  • J. Lademann
  • S. Lange-Asschenfeldt
  • M. Ulrich
  • M. E. Darvin
  • K. König
  • M. C. Meinke
  • B. Lange-Asschenfeldt
Chapter

Abstract

The skin is not only the largest organ of the human body but also represents a barrier to the environment, protecting our body from water loss, penetration of environmental pollutants and microorganisms [1–3]. The skin has also a social function, as people frequently associate success in their private and professional lives with young-looking, smooth skin [4]. Therefore, not only medical but also cosmetic aspects are to be considered in skin treatment. While topically applied medical products must penetrate through the skin barrier in order to become effective in the living cells, cosmetic products do not necessarily induce any medical effects [5]. In most cases, these products remain on the skin surface or in the upper corneal layers in order to protect the skin (e.g., sunscreens) or to stabilise the dermal barrier (e.g., skin protection creams or lotions). Sometimes, in particular with respect to anti-aging creams, it is difficult to distinguish medical from cosmetic products.

Keywords

Stratum Corneum Hair Follicle Laser Scanning Microscopy Basal Cell Carcinoma Actinic Keratosis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. 1.
    Schittek B (2011) The antimicrobial skin barrier in patients with atopic dermatitis. Curr Probl Dermatol 41:54–67PubMedGoogle Scholar
  2. 2.
    Seyfarth F, Schliemann S, Antonov D, Elsner P (2011) Dry skin, barrier function, and irritant contact dermatitis in the elderly. Clin Dermatol 29(1):31–36PubMedCrossRefGoogle Scholar
  3. 3.
    Lademann J, Richter H, Schanzer S, Knorr F, Meinke M, Sterry W, Patzelt A (2011) Penetration and storage of particles in human skin: perspectives and safety aspects. Eur J Pharm Biopharm 77(3):465–468PubMedCrossRefGoogle Scholar
  4. 4.
    Martin KI, Glaser DA (2011) Cosmeceuticals: the new medicine of beauty. Mo Med 108(1):60–63PubMedGoogle Scholar
  5. 5.
    Situm M, Bulat V, Buljan M, Puljiz Z, Situm V, Bolanca Z (2010) Senile lentigo – cosmetic or medical issue of the elderly population. Coll Antropol 34(Suppl 2):85–88PubMedGoogle Scholar
  6. 6.
    Lademann J, Jacobi U, Surber C, Weigmann HJ, Fluhr JW (2009) The tape stripping procedure–evaluation of some critical parameters. Eur J Pharm Biopharm 72(2):317–323PubMedCrossRefGoogle Scholar
  7. 7.
    Wei H, Chen Y, Xu L, Zheng J (2007) Percutaneous penetration kinetics of lidocaine and prilocaine in two local anesthetic formulations assessed by in vivo microdialysis in pigs. Biol Pharm Bull 30(4):830–834PubMedCrossRefGoogle Scholar
  8. 8.
    Lademann J, Richter H, Schaefer UF, Blume-Peytavi U, Teichmann A, Otberg N, Sterry W (2006) Hair follicles – a long-term reservoir for drug delivery. Skin Pharmacol Physiol 19(4):232–236PubMedCrossRefGoogle Scholar
  9. 9.
    Lademann J, Otberg N, Richter H, Meyer L, Audring H, Teichmann A, Thomas S, Knuttel A, Sterry W (2007) Application of optical non-invasive methods in skin physiology: a comparison of laser scanning microscopy and optical coherent tomography with histological analysis. Skin Res Technol 13(2):119–132PubMedCrossRefGoogle Scholar
  10. 10.
    Roberts MS, Dancik Y, Prow TW, Thorling CA, Lin LL, Grice JE, Robertson TA, Konig K, Becker W (2011) Non-invasive imaging of skin physiology and percutaneous penetration using fluorescence spectral and lifetime imaging with multiphoton and confocal microscopy. Eur J Pharm Biopharm 77(3):469–488PubMedCrossRefGoogle Scholar
  11. 11.
    Meyer LE, Otberg N, Sterry W, Lademann J (2006) In vivo confocal scanning laser microscopy: comparison of the reflectance and fluorescence mode by imaging human skin. J Biomed Opt 11(4):044012PubMedCrossRefGoogle Scholar
  12. 12.
    Sattler E, Kastle R, Arens-Corell M, Welzel J (2012) How long does protection last? – In vivo fluorescence confocal laser scanning imaging for the evaluation of the kinetics of a topically applied lotion in an everyday setting. Skin Res Technol 18(3):370–377PubMedCrossRefGoogle Scholar
  13. 13.
    Astner S, Dietterle S, Otberg N, Rowert-Huber HJ, Stockfleth E, Lademann J (2008) Clinical applicability of in vivo fluorescence confocal microscopy for noninvasive diagnosis and therapeutic monitoring of nonmelanoma skin cancer. J Biomed Opt 13(1):014003PubMedCrossRefGoogle Scholar
  14. 14.
    Konig K (2008) Clinical multiphoton tomography. J Biophotonics 1(1):13–23PubMedCrossRefGoogle Scholar
  15. 15.
    Larsen TH, Gregersen P, Jemec GB (2001) Skin irritation and exposure to diisocyanates in orthopedic nurses working with soft casts. Am J Contact Dermat 12(4):211–214PubMedCrossRefGoogle Scholar
  16. 16.
    Laudanska H, Reduta T, Szmitkowska D (2003) Evaluation of skin barrier function in allergic contact dermatitis and atopic dermatitis using method of the continuous TEWL measurement. Rocz Akad Med Bialymst 48:123–127PubMedGoogle Scholar
  17. 17.
    Pinnagoda J, Tupker RA, Agner T, Serup J (1990) Guidelines for transepidermal water loss (TEWL) measurement. A report from the Standardization Group of the European Society of Contact Dermatitis. Contact Dermatitis 22(3):164–178PubMedCrossRefGoogle Scholar
  18. 18.
    Vergou T, Schanzer S, Richter H, Pels R, Thiede G, Patzelt A, Meinke MC, Sterry W, Fluhr JW, Lademann J (2012) Comparison between TEWL and laser scanning microscopy measurements for the in vivo characterization of the human epidermal barrier. J Biophotonics 5(2):152–158PubMedCrossRefGoogle Scholar
  19. 19.
    Teichmann A, Sadey Pour Saleh H, Schanzer S, Richter H, Lademann J (2006) Investigations of bedan face cream. Kosmetische Medizin 4:180–182Google Scholar
  20. 20.
    Weigmann HJ, Schanzer S, Teichmann A, Durat F, Antoniou C, Schaefer H, Sterry W, Lademann J (2007) Ex-vivo spectroscopic quantification of sunscreen efficacy: proposal of a universal sun protection factor. J Biomed Opt 12(4):044013PubMedCrossRefGoogle Scholar
  21. 21.
    Bal SM, Kruithof AC, Zwier R, Dietz E, Bouwstra JA, Lademann J, Meinke MC (2010) Influence of microneedle shape on the transport of a fluorescent dye into human skin in vivo. J Control Release 147(2):218–224PubMedCrossRefGoogle Scholar
  22. 22.
    Blume-Peytavi U, Massoudy L, Patzelt A, Lademann J, Dietz E, Rasulev U, Garcia Bartels N (2010) Follicular and percutaneous penetration pathways of topically applied minoxidil foam. Eur J Pharm Biopharm 76(3):450–453PubMedCrossRefGoogle Scholar
  23. 23.
    Knorr F, Lademann J, Patzelt A, Sterry W, Blume-Peytavi U, Vogt A (2009) Follicular transport route–research progress and future perspectives. Eur J Pharm Biopharm 71(2):173–180PubMedCrossRefGoogle Scholar
  24. 24.
    Leclerc EA, Huchenq A, Mattiuzzo NR, Metzger D, Chambon P, Ghyselinck NB, Serre G, Jonca N, Guerrin M (2009) Corneodesmosin gene ablation induces lethal skin-barrier disruption and hair-follicle degeneration related to desmosome dysfunction. J Cell Sci 122(Pt 15):2699–2709PubMedCrossRefGoogle Scholar
  25. 25.
    Patzelt A, Knorr F, Blume-Peytavi U, Sterry W, Lademann J (2008) Hair follicles, their disorders and their opportunities. Drugs Discov Today 5(2):e173–e181CrossRefGoogle Scholar
  26. 26.
    Patzelt A, Richter H, Buettemeyer R, Huber HJ, Blume-Peytavi U, Sterry W, Lademann J (2008) Differential stripping demonstrates a significant reduction of the hair follicle reservoir in vitro compared to in vivo. Eur J Pharm Biopharm 70(1):234–238PubMedCrossRefGoogle Scholar
  27. 27.
    Elias PM, Steinhoff M (2008) “Outside-to-inside” (and now back to “outside”) pathogenic mechanisms in atopic dermatitis. J Invest Dermatol 128(5):1067–1070PubMedCrossRefGoogle Scholar
  28. 28.
    Jacobi U, Meykadeh N, Sterry W, Lademann J (2003) Effect of the vehicle on the amount of stratum corneum removed by tape stripping. J Dtsch Dermatol Ges 1(11):884–889PubMedCrossRefGoogle Scholar
  29. 29.
    Lademann J, Ilgevicius A, Zurbau O, Liess HD, Schanzer S, Weigmann HJ, Antoniou C, Pelchrzim RV, Sterry W (2006) Penetration studies of topically applied substances: optical determination of the amount of stratum corneum removed by tape stripping. J Biomed Opt 11(5):054026PubMedCrossRefGoogle Scholar
  30. 30.
    Meinke M, Abdollahnia M, Gahr F, Platzek T, Sterry W, Lademann J (2009) Migration and penetration of a fluorescent textile dye into the skin–in vivo versus in vitro methods. Exp Dermatol 18(9):789–792PubMedCrossRefGoogle Scholar
  31. 31.
    Weigmann H, Lademann J, Meffert H, Schaefer H, Sterry W (1999) Determination of the horny layer profile by tape stripping in combination with optical spectroscopy in the visible range as a prerequisite to quantify percutaneous absorption. Skin Pharmacol Appl Skin Physiol 12(1–2):34–45PubMedGoogle Scholar
  32. 32.
    Feldmann RJ, Maibach HI (1969) Percutaneous penetration of steroids in man. J Invest Dermatol 52(1):89–94PubMedGoogle Scholar
  33. 33.
    Baroli B (2010) Penetration of nanoparticles and nanomaterials in the skin: fiction or reality? J Pharm Sci 99(1):21–50PubMedCrossRefGoogle Scholar
  34. 34.
    Burnett ME, Wang SQ (2011) Current sunscreen controversies: a critical review. Photodermatol Photoimmunol Photomed 27(2):58–67PubMedCrossRefGoogle Scholar
  35. 35.
    Johnson JC, Yan H, Schaller RD, Petersen PB, Yang PD, Saykally RJ (2002) Near-field imaging of nonlinear optical mixing in single zinc oxide nanowires. Nano Lett 2(4):279–283CrossRefGoogle Scholar
  36. 36.
    Segets D, Tomalino LM, Gradl J, Peukert W (2009) Real-time monitoring of the nucleation and growth of ZnO nanoparticles using an optical hyper-rayleigh scattering method. J Phys Chem C 113(28):11995–12001CrossRefGoogle Scholar
  37. 37.
    Darvin ME, Konig K, Kellner-Hofer M, Breunig HG, Werncke W, Meinke MC (2012) Safety assessment by multiphoton fluorescence/SHG/HRS tomography of ZnO nanoparticles used in cosmetic products. Skin Pharmacol Physiol 25(4):219–226PubMedCrossRefGoogle Scholar
  38. 38.
    Lademann O, Richter H, Kramer A, Patzelt A, Meinke MC, Graf C, Gao Q, Korotianskiy E, Rühl E, Weltmann KD, Lademann J, Koch S (2011) Stimulation of the penetration of particles into the skin by plasma tissue interaction. Laser Phys Lett 8(10):758–764CrossRefGoogle Scholar

Copyright information

© Springer Berlin Heidelberg 2014

Authors and Affiliations

  • J. Lademann
    • 1
  • S. Lange-Asschenfeldt
    • 2
  • M. Ulrich
    • 2
  • M. E. Darvin
    • 1
  • K. König
    • 3
    • 4
  • M. C. Meinke
    • 1
  • B. Lange-Asschenfeldt
    • 2
  1. 1.Department of Dermatology, Center of Experimental and Applied Cutaneous Physiology, Venerology and AllergologyCharité – Universitätsmedizin BerlinBerlinGermany
  2. 2.Department of Dermatology, Skin Cancer CenterCharité – Universitätsmedizin BerlinBerlinGermany
  3. 3.JenLab GmbHJenaGermany
  4. 4.Department of Biophotonics and Laser TechnologySaarland UniversitySaarbrückenGermany

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