D1′—a New Member of D1 Protein Family in Cyanobacteria

  • Ciprian Chis
  • Abdulah Mahboob
  • Sergey Vassilieev
  • Adriana Bica
  • Loredana Peca
  • Doug Brouce
  • Eva-Mari Aro
  • Cosmin Ionel Sicora
Part of the Advanced Topics in Science and Technology in China book series (ATSTC)

Abstract

The D1 protein of Photosystem II (PSII), encoded by the psbA genes, is an indispensable component of oxygenic photosynthesis. Due to strongly oxidative chemistry of PSII water splitting, the D1 protein is prone to constant photodamage requiring its replacement, whereas most of the other PSII subunits remain ordinarily undamaged. In cyanobacteria, the D1 protein is encoded by apsbA gene family, whose members are differentially expressed according to environmental cues. In the recent years many cyanobacterial genomes have been sequenced and are available on the Internet. We have constructed a database of the D1 protein sequences and run several phylogenetic studies in an effort to elucidate the structure of the psbA gene families for those species where the functional studies are missing. Also, we present data regarding the change in psbA gene family expression under environmental stress. A general classification of cyanobacterial D1 isoforms in various cyanobacterial species into D1m, D1:1, D1:2, and D10 forms depending on their expression pattern under acclimated growth conditions and upon stress is discussed, taking into consideration the phototolerance of different D1 forms and the expression conditions of respective members of the psbA gene family.

Keywords

D1 Photosystem II Cyanobacteria 

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References

  1. Aro EM, Virgin I, Andersson B Photoinhibition of Photosystem II. Inactivation, Protein Damage and Turnover Biochim. Biophys. Acta 1143: 113–134Google Scholar
  2. Kos PB, Deak Z, Cheregi O, Vass I (2008) Differential Regulation of psbA and psbD Gene Expression, and the Role of the Different D1 Protein Copies in the Cyanobacterium Thermosynechococcus Elongatus BP-1. Biochim. Biophys. Acta 1777: 74–83PubMedCrossRefGoogle Scholar
  3. Kulkarni RD, Golden SS (1994) Adaptation to High Light-Intensity in Synechococcus sp. Strain PCC-7942 — Regulation of 3 psbA Genes and 2 Forms of the D1 Protein. J. Bact. 176: 959–965PubMedGoogle Scholar
  4. Sicora CI, Appleton SE, Brown CM, Chung J, Chandler J, Cockshutt AM, Vass I, Campbell DA (2006) Cyanobacterial psbA Families in Anabaena and Synechocystis Encode Trace, Constitutive and UVB-Induced D1 Isoforms. Biochim. Biophys. Acta 1757: 47–56PubMedCrossRefGoogle Scholar
  5. Sicora CI, Brown CM, Cheregi O, Vass I, Campbell DA (2008) The psbA Gene Family Responds Differentially to Light and UVB Stress in Gloeobacter Violaceus PCC 7421, a Deeply Divergent Cyanobacterium. Biochim. Biophys. Acta 1777: 130–139PubMedCrossRefGoogle Scholar
  6. Vass I, Szilard A, Sicora C (2005) Adverse Effects of UV-B Light on the Structure and Function of the Photosynthetic Apparatus. In: M Pessarakli (ed.), Handbook of Photosynthesis, 2nd ed., Marcel Dekker, Inc.: New York, pp. 827–843Google Scholar

Copyright information

© Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Ciprian Chis
    • 1
    • 3
  • Abdulah Mahboob
    • 2
  • Sergey Vassilieev
    • 2
  • Adriana Bica
    • 3
  • Loredana Peca
    • 3
  • Doug Brouce
    • 2
  • Eva-Mari Aro
    • 4
  • Cosmin Ionel Sicora
    • 1
    • 3
  1. 1.Biological Research Center JibouRomania
  2. 2.Department of Biological SciencesBrock UniversitySt. CatharinesCanada
  3. 3.Biological Research Institute Cluj-NapocaRomania
  4. 4.Department of Biology, Plant Physiology and Molecular BiologyUniversity of TurkuFinland

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