Stromatolites are sedimentary structures formed by microbial mats that are typically found in limestone-or dolostone-rich environments. Shark Bay, Australia, has abundant examples of living marine stromatolites. Although the stromatolites from Shark Bay are only about 2000–3000 years old, they are similar to fossilized evidence of life found on Earth up to 3.5 billion years ago. Using infra-red light centred at 720 nm, new chlorophyll d-containing microorganisms were isolated from the living stromatolites (collected from Shark Bay, Western Australia) and red algae on mangrove pneumatophores (collected from the Georges River, Sydney, Australia) and enriched in KES+ seawater medium. Microscopic examination of the red-light enriched cultures confirmed that they are Acaryochloris-like cyanobacteria. Using cyanobacterial-specific 16S rRNA gene primers, we obtained almost full length sequences of 16S rDNA from the newly isolated Chl d-containing cyanobacteria. The sequences shared 98% identity with Acaryochloris marina MBIC11017. Interestingly, the strain isolated from stromatolites (designated as “ssball1” strain) was more similar to Acaryochloris sp CR111A while the strain isolated from Georges River (designated as “Mangrove1” strain) was more closely related to Acaryochloris sp CCMEE 5401, which was isolated from an inland “lake”, Salton Sea in California,. Pigment composition of the newly isolated strains were determined using HPLC, However, no obvious differences were noted. Chl d was the major photopigment while Chl a was present as a minor photopigment, about 2%–3.5 % of the total chlorophyll.
- Acaryochloris marina
- Chlorophyll d
- Chlorophyll d-containing cyanobacteria
- 16S rRNA classification
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Behrendt L, Trampe E, Larkum AWD, Norman A, Qvortrup K, Chen M, Ralph P, Sørensen SJ, Kühl M (2011) Endolithic Chlorophyll d Containing Phototrophs. ISME J (in press, doi: 10.1038/ismej.2010.195).
Chen M, Schliep M, Willows RD, Cai ZL, Neilan BA, Scheer H (2010) A Red-Shifted Chlorophyll. Science 329: 1318–1319
Duxbury Z, Schliep M, Ritchie RJ, Larkum AWD, Chen M (2009) Chromatic Photoacclimation Extends Utilisable Photosynthetically Active Radiation in the Chlorophyll d-Containing Cyanobacterium, Acaryochloris Marina. Photosynth. Res. 101:69–75
Jungblut A, Hawes I, Mountfort D, Hitzfeld B, Dietrich DR, Burns BP, Neilan BA (2005) Diversity within Cyanobacterial Mat Communities in Variable Salinity Meltwater Ponds of McMurdo Ice Shelf, Antarctica, Environ. Microbiol. 7: 519–529
Kashiyama Y, et al. (2008) Evidence for Global Chlorophyll d. Science 321: 658–658
Kühl M, Chen M, Ralph PJ, Schreiber U, Larkum AWD (2005) A Niche for Cyanobacteria Containing Chlorophyll d. Nature 433: 820–820
Kühl M, Chen M, Larkum AWD (2007) Biology of the Chlorophyll d-Containing Cyanobacterium Acaryochloris Marina. In: J Seckbach (ed.), Algae and Cyanobacteria in Extreme Environments. Springer: Berlin, pp. 101–123
Miller SR, et al. (2005) Discovery of a Free-Living Chlorophyll d-Producing Cyanobacterium with a Hybrid Proteobacterial/Cyanobacterial Small-Subunit rRNA Gene. Proc. Natl. Acad. Sci. USA 102: 850–855
Miyashita H, Ikemoto H, Kurano N, Adachi K, Chihara M, Miyachi S (1996) Chlorophyll d As a Major Pigment. Nature 383: 402–402
Miyashita H, Adachi K, Miyachi S, Chihara M, Ikemoto H, Kurano N (1997) Pigment Composition of a Novel Oxygenic Photosynthetic Prokaryote Containing Chlorophyll d As the Major Chlorophyll. Plant and Cell Physiology, 38: 274–281
Mohr R, Voss B, Schliep M, Kurz T, Maldener I, Adas DG, Larkum AWD, Chen M, Hess WR (2010) Niche Adaptation in a New Chlorophyll d-Containing Cyanobacterium from the Genus Acaryochloris. ISME J 4: 1456–1469
Neilan BA, Jacobs D, DelDot T, Blackall LL, Hawkins PR, Cox PT, Goodman AE (1997) rRNA Sequences and Evolutionary Relationships among Toxic and Nontoxic Cyanobacteria of the Genus Microcystis. International Journal of Systematic Bacteriology 47: 693–697
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Li, Y., Larkum, A., Schliep, M., Kühl, M., Neilan, B., Chen, M. (2013). Newly Isolated Chl d-Containing Cyanobacteria. In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_148
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