Abstract
Thin-layer cell spectroelectrochemistry was applied for the first time to measure the redox potentials of pheophytin (Phe) a and the primary quinone QA in photosystem (PS) II core complexes from a thermophilic cyanobacterium Thermosynechococcus elongatus. The determined potentials for oxygen-evolving PS II from a T. elongatus wild type strain were −522 ± 3 mV and −140 ± 2 mV for Phe a and QA, respectively. Based on the determined values together with kinetic analytical data in literature, a renewed diagram is proposed for the energetics in PS II, and further, modification of the redox properties for acclimation to cultivate conditions is also discussed.
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References
Dau H, Sauer K (1996) Exciton Equilibration and Photosystem II Exciton Dynamics — a Fluorescence Study on Photosystem II Membrane Particles of Spinach. Biochim. Biophys. Acta 1273: 175–190
Giorgi LB, Nixon PJ, Merry SAP, Joseph DM, Durrant JR, Rivas JDL, Barber J, Porter G, Klug DR (1996) Comparison of Primary Charge Separation in the Photosystem II Reaction Center Complex Isolated from Wild-Type and D1-130 Mutants of the Cyanobacterium Synechocystis PCC 6803. J. Biol. Chem. 271: 2093–2101
Grabolle M, Dau H (2005) Energetics of Primary and Secondary Electron Transfer in Photosystem II Membrane Particles of Spinach Revisited on Basis of Recombination-Fluorescence Measurements. Biochim. Biophys. Acta 1708: 209–218
Hawkridge FM, Ke B (1977) An Electrochemical Thin-Layer Cell for Spectroscopic Studies of Photosynthetic Electron-Transport Components. Anal. Biochem. 78: 76–85
Johnson GN, Rutherford AW, Krieger A (1995) A Change in the Midpoint Potential of the Quinone QA in Photosystem II Associated with Photoactivation of Oxygen Evolution. Biochim. Biophys. Acta 1229: 202–207
Kato Y Sugiura, M Oda A, Watanabe T (2009) Spectroelectrochemical Determination of the Redox Potential of Pheophytin a, the Primary Electron Acceptor in Photosytem II. Proc. Natl. Acad. Sci. USA 106: 17365–17370
Klimov VV, Allakhverdiev SI, Pashchenko VZ (1978) Measurement of the Activation Energy and Lifetime of Fluorescence of Photosystem 2 Chlorophyll. Dokl. Akad. Nauk SSSR 242: 1204–1207
Klimov VV, Allakhverdiev SI, Demeter S, Krasnovskii AA (1979) Photoreduction of Pheophytin in the Photosystem 2 of Chloroplasts with Respect to the Redox Potential of the Medium. Dakl. Akad. Nauk SSSR 249: 227–30
Kos PB, Deak Z, Cheregi O, Vass I (2008) Differential Regulation of psbA and psbD Gene Expression, and the Role of the Different D1 Protein Copies in the Cyanobacterium Thermosynechococcus Elongatus BP-1. Biochim. Biophys. Acta 1777: 74–83
Krieger A, Rutherford AW, Johnson GN (1995) On the Determination of Redox Midpoint Potential of the Primary Quinone Electron Acceptor, QA, in Photo-System II. Biochim. Biophys. Acta 1229: 193–201
Minagawa K, Narusaka Y, Inoue Y, Satoh K (1999) Electron Transfer between QA and QB in Photosystem II is Thermodynamically Perturbed in Phototolerant Mutants of Synechocystis sp. PCC 6803. Biochemistry 38: 770–775
Rappaport F, Guervova-Kuras M, Nixon PJ, Diner BA, Lavergne J (2002) Kinetics and Pathways of Charge Recombination in Photosystem II. Biochemistry 41: 8518–8527
Renger G (2008) Functional Patter of Photosystem II. In: Renger G (ed.), Primary Processes of Photosynthesis: Principles and Apparatus, Part II. Royal Society Chemistry: Cambridge, pp. 237–290
Rutherford AW, Mullet JE, Crofts AR (1981) Measurement of the Midpoint Potential of the Pheophytin Acceptor of Photosystem II. FEBS Lett. 123:235–237
Shibamoto T, Kato Y, Sugiura M, Watanabe T (2009) Redox Potential of the Primary Plastoquinone Electron Acceptor QA in Photosystem II from Thermo-Synechococcus Elongatus Determined by Spectro-Electrochemistry. Biochemistry 48: 10682–10684
Shibuya Y, Takahashi R, Okubo T, Suzuki H, Sugiura M, Noguchi T (2010) Hydrogen Bond Interaction of the Pheophytin Electron Acceptor and Its Radical Anion in Photosystem II as Revealed by Fourier Transform Infrared Difference Spectroscopy. Biochemistry 49: 493–501
Sugiura M, Inoue Y (1999) Highly Purified Thermo-Stable Oxygen-Evolving Photosystem II Core Complex from the Thermophilic Cyanobacterium Synechococcus Elongatus Having His-Tagged CP43. Plant Cell Physiol. 40: 1219–1231
Sugiura M, Kato Y, Takahashi R, Suzuki H, Watanabe T, Noguchi T, Rappaport F, Boussac A (2010) Energetics in Photosystem II from Thermosynechococcus Elongatus with a D1 Protein Encoded by Either the psbA1 or psbA3 gene. Biochim. Biophys. Acta 1797: 1491–1499
Weller A (1982) Photoinduced Electron Transfer in Solution: Exciplex and Radical Ion Pair Formation Free Enthalpies and Their Solvent Dependence. Z. Phys. Chem. 133: 93–98
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© 2013 Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg
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Kato, Y., Shibamoto, T., Oda, A., Sugiura, M., Watanabe, T. (2013). Spectroelectrochemical Determination of the Redox Potentials of Pheophytin a and Primary Quinone QA in Photosystem II from Thermosynechococcus Elongatus . In: Photosynthesis Research for Food, Fuel and the Future. Advanced Topics in Science and Technology in China. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-32034-7_10
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DOI: https://doi.org/10.1007/978-3-642-32034-7_10
Publisher Name: Springer, Berlin, Heidelberg
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