D-Xylitol pp 245-263 | Cite as

Current Analytical Methods for Qualitative and Quantitative Measurement of d-Xylitol

  • Zhongqi Jiang
  • Samuel Amartey
  • Zi-Hua Jiang
  • Wensheng QinEmail author


d-xylitol is a naturally-occurring five-carbon sugar alcohol. It can also be derived from the chemical reduction of d-xylose. It is widely used in recent years and will continue to be used as a food additive and sweetening agent in the food industry. The qualitative detection and quantification of d-xylitol in the presence of other sugars and sugar alcohols in fruits, vegetables and other natural sources is essential for industry production. A number of analytical methods have been developed over the years for qualitative detection and quantitative measurement of d-xylitol. Since most samples to be analyzed contain a mixture of compounds, highly efficient and sensitive analytical methods for d-xylitol in the mixture are required. Current analytical methods are usually comprised of two components: (1) an efficient separation unit, and (2) a structure identification unit. In this chapter, we provide an overview on these analytical methods used for the qualitative and quantitative determination of d-xylitol in samples from various sources. Chromatography-based techniques including GC, HPLC and CE methods with different detection options, such as UV, RI, ELS, etc., have been widely used. More advanced analytical instruments derived from hyphenation of chromatography with structure determination tools such as MS and NMR are becoming more and more accessible. The GC–MS, LC–MS and LC–MS/MS have now become routine methods for d-xylitol measurement. The coupling of spectroscopic methods such as NMR and MS to the chromatography methods can also provide structural information of the compounds being analyzed. Other methods such as the immunoassay and enzymatic assay methods are also discussed.


d-xylitol Analytical method Detection method Chromatographic separation Spectroscopy Biosensor 



Proton nuclear magnetic resonance


Bovine serum albumin


Capillary electrophoresis


Capillary zone electrophoresis


Enzyme-linked immunosorbent assay


Evaporative light-scattering


Electrospray ionization mass spectrometry


Flow injection analyisis


Flame Ionization Detector


Fourier transform ion cyclotron resonance


Gas chromatography


Gas chromatography-flame ionization detector


Gas chromatography-mass spectrometry


High-pH anion exchange chromatography


High-performance liquid chromatography


Indirect competitive enzyme-linked immunosorbant assay


1-isopropyl-3-(3-dimethylaminopropyl) carbodiimide perchlorate

Ig E

Immunoglobulin E

Ig G

Immunoglobulin G


Internal standard


Capillary isotachophoresis


Liquid chromatography-mass spectrometry


Liquid chromatography-nuclear magnetic resonance


Mass spectrometry


Nicotinamide adenine dinucleotide


Nicotinamide adenine dinucleotide phosphate


Nuclear magnetic resonance


Optimum performance laminar chromatography


Pulsed amperometric detector


Refractive index


Relative standard deviation




Thin layer chromatography




d-xylitol dehydrogenase


d-xylitol oxidase


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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Zhongqi Jiang
    • 1
  • Samuel Amartey
    • 2
  • Zi-Hua Jiang
    • 3
  • Wensheng Qin
    • 4
    Email author
  1. 1.College of Animal SciencesZhejiang UniversityHangzhouPeople’s Republic of China
  2. 2.Division of BiologyImperial College of Science, Technology and MedicineSouth KensingtonUK
  3. 3.Department of ChemistryLakehead UniversityThunder BayCanada
  4. 4.Department of Biology & Biorefining Research InstituteLakehead UniversityThunder BayCanada

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