Recombinant Allergens and Applications

  • Ying-Tao Ma
  • Zhao-Wei Yang
  • Zhong-Shan Gao
Part of the Advanced Topics in Science and Technology in China book series (ATSTC)


Recombinant DNA technology has great potential in various aspects of allergen-related research and clinical applications. Sufficient amounts of purified wild-type or immunologically modified allergens or fragments have been produced in heterologous expression systems for use in many research fields, such as molecular characterization of the allergen (e.g., three-dimensional structure and epitope mapping), allergen standardization, component-resolved diagnosis (CRD) and patient-tailored specific immunotherapy (SIT). Strategies for obtaining recombinant allergens generally involve three steps, with the choice of heterologous expression system, bacteria, yeast, insect or plant cell, and the purification methods being of major importance. Here we review the major methods used for determining the three-dimensional structure and for epitope mapping, the recent progress in the application of recombinant allergens in clinical research of allergenic disease, such as the recombinant allergen-based microarray diagnosis and therapeutic vaccine.


Recombinant Allergen Natural Counter Recombinant Aller 
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Copyright information

© Zhejiang University Press, Hangzhou and Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Ying-Tao Ma
    • 1
  • Zhao-Wei Yang
    • 1
  • Zhong-Shan Gao
    • 1
  1. 1.Allergy Research Center/Department of HorticultureZhejiang UniversityHangzhouChina

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