Proteomic Investigations for Boron Neutron Capture Therapy
Proteomic investigations are of primary importance for discovery-driven biomarker studies. In fact, for understanding the biochemical and physiological differences between tumor and normal cells/tissues, and for using these differences in compound design, synthesis, and targeting, it is important to investigate protein profiles related to physiological and pathological conditions, and to describe the effect of bioactive compounds. Today, different proteomic methodologies are available for clinical proteomic applications. In this chapter, the main steps of traditional two-dimensional gel electrophoresis (2DE) and innovative multidimensional protein identification (MudPIT) are described, which are useful for BNCT investigations. In particular, some results concerning the possibility of characterizing proteins interacting with 10boron compounds and shotgun proteomic analysis of tumor tissues for biomarker discovery using a mass spectrometry-based approach are described.
KeywordsBoron Neutron Capture Therapy Proteomic Investigation Experimental Mass Spectrum Phospholipid Hydroperoxide Glutathione Peroxidase Surface Enhance Laser Desorption
Polyvinylidene fluoride transfer membranes
Multidimensional protein identification technology
Liquid tandem mass spectrometry
Immobilized pH gradient
Sodium dodecyl sulfate-polyacrylamide gel electrophoresis
The authors are grateful to Dr. A. Roveri and Dr. S. Altieri for the PHGPx reaction with BSH and neutron autoradiography, respectively.
- 17.Mauri P, Scarpa A, Nascimbeni AC, Benazzi L, Parmagnani E, Mafficini A, Della Peruta M, Bassi C, Miyazaki K, Sorio C (2005) Identification of proteins released by pancreatic cancer cells by multidimensional protein identification technology: a strategy for identification of novel cancer markers. FASEB J 19(9):1125–1127PubMedGoogle Scholar
- 19.Sodek KL, Evangelou AI, Ignatchenko A, Agochiya M, Brown TJ, Ringuette MJ, Jurisica I, Kislinger T (2008) Identification of pathways associated with invasive behavior by ovarian cancer cells using multidimensional protein identification technology (MudPIT). Mol Biosyst 4(7):762–773PubMedCrossRefGoogle Scholar
- 22.Mauri PL, Basilico F, Wittig A, Heimans J, Sauerwein W (2006). Pharmacokinetics and metabolites of 10B-contaning compounds in biological fluids. In: Oral presentation. 12th ICNCT, Kagawa, 9 Oct 2006Google Scholar
- 24.Doi A, Kawabata S, Iida K, Yokoyama K, Kajimoto Y, Kuroiwa T, Shirakawa T, Kirihata M, Kasaoka S, Maruyama K, Kumada H, Sakurai Y, Masunaga S, Ono K, Miyatake S (2008) Tumor-specific targeting of sodium borocaptate (BSH) to malignant glioma by transferrin-PEG liposomes: a modality for boron neutron capture therapy. J Neurooncol 87(3):287–294PubMedCrossRefGoogle Scholar