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Proteomic Investigations for Boron Neutron Capture Therapy

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Neutron Capture Therapy

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Abstract

Proteomic investigations are of primary importance for discovery-driven biomarker studies. In fact, for understanding the biochemical and physiological differences between tumor and normal cells/tissues, and for using these differences in compound design, synthesis, and targeting, it is important to investigate protein profiles related to physiological and pathological conditions, and to describe the effect of bioactive compounds. Today, different proteomic methodologies are available for clinical proteomic applications. In this chapter, the main steps of traditional two-dimensional gel electrophoresis (2DE) and innovative multidimensional protein identification (MudPIT) are described, which are useful for BNCT investigations. In particular, some results concerning the possibility of characterizing proteins interacting with 10boron compounds and shotgun proteomic analysis of tumor tissues for biomarker discovery using a mass spectrometry-based approach are described.

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Abbreviations

BSH:

Sodium mercaptoundecahydro-closo-dodecaborate

BPA:

L-para-boronophenylalanine

PVDF:

Polyvinylidene fluoride transfer membranes

MudPIT:

Multidimensional protein identification technology

LC-MS/MS:

Liquid tandem mass spectrometry

IPG:

Immobilized pH gradient

SDS-PAGE:

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

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Acknowledgements

The authors are grateful to Dr. A. Roveri and Dr. S. Altieri for the PHGPx reaction with BSH and neutron autoradiography, respectively.

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Authors and Affiliations

  1. Proteomics and Metabolomics Unit, Institute for Biomedical Technologies (ITB-CNR), Via Fratelli Cervi, 93, 20090, Segrate Milan, Italy

    Pier Luigi Mauri & Fabrizio Basilico

Authors
  1. Pier Luigi Mauri
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  2. Fabrizio Basilico
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Correspondence to Pier Luigi Mauri .

Editor information

Editors and Affiliations

  1. Zentrum für Konservative Onkologie, Klinik für Strahlentherapie, Univ.Klinikum Essen, Hufelandstr. 55, Essen, 45122, Germany

    Wolfgang Sauerwein

  2. Philipps-University Marburg, Department of Radiation Oncology, University Hospital Marburg,, Baldingerstrasse 1, Marburg, 35043, Germany

    Andrea Wittig

  3. Joint Research Centre, Institute for Engergy, European Commission, Westerduinweg 3, LE Petten, 1755, Netherlands

    Raymond Moss

  4. , Department of Neurosurgery, Kagawa National Children's Hospital, Zentsuji-cho 2603, Zentsuji, 765-8501, Kagawa, Japan

    Yoshinobu Nakagawa

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Mauri, P.L., Basilico, F. (2012). Proteomic Investigations for Boron Neutron Capture Therapy. In: Sauerwein, W., Wittig, A., Moss, R., Nakagawa, Y. (eds) Neutron Capture Therapy. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-31334-9_10

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  • DOI: https://doi.org/10.1007/978-3-642-31334-9_10

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  • Print ISBN: 978-3-642-31333-2

  • Online ISBN: 978-3-642-31334-9

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