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Proteomic Investigations for Boron Neutron Capture Therapy

  • Pier Luigi Mauri
  • Fabrizio Basilico
Chapter

Abstract

Proteomic investigations are of primary importance for discovery-driven biomarker studies. In fact, for understanding the biochemical and physiological differences between tumor and normal cells/tissues, and for using these differences in compound design, synthesis, and targeting, it is important to investigate protein profiles related to physiological and pathological conditions, and to describe the effect of bioactive compounds. Today, different proteomic methodologies are available for clinical proteomic applications. In this chapter, the main steps of traditional two-dimensional gel electrophoresis (2DE) and innovative multidimensional protein identification (MudPIT) are described, which are useful for BNCT investigations. In particular, some results concerning the possibility of characterizing proteins interacting with 10boron compounds and shotgun proteomic analysis of tumor tissues for biomarker discovery using a mass spectrometry-based approach are described.

Keywords

Boron Neutron Capture Therapy Proteomic Investigation Experimental Mass Spectrum Phospholipid Hydroperoxide Glutathione Peroxidase Surface Enhance Laser Desorption 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

BSH

Sodium mercaptoundecahydro-closo-dodecaborate

BPA

L-para-boronophenylalanine

PVDF

Polyvinylidene fluoride transfer membranes

MudPIT

Multidimensional protein identification technology

LC-MS/MS

Liquid tandem mass spectrometry

IPG

Immobilized pH gradient

SDS-PAGE

Sodium dodecyl sulfate-polyacrylamide gel electrophoresis

Notes

Acknowledgements

The authors are grateful to Dr. A. Roveri and Dr. S. Altieri for the PHGPx reaction with BSH and neutron autoradiography, respectively.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Proteomics and Metabolomics UnitInstitute for Biomedical Technologies (ITB-CNR)Segrate MilanItaly

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