Abstract
The internal storage mechanisms and electron flow from the external substrate occurring in the aerobic granule sludge under both aerobic and anoxic conditions were explored with extensive storage experiments. The simultaneous growth and storage processes in aerobic granules were accurately modeled. It was found that the aerobic granules in an SBR, subjected to alternative feast and famine conditions, were able to rapidly take up carbon substrate in wastewater and to store it as intracellular storage products when the substrate was in excess.
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Almeida, J.S., Reis, M.A.M., Carrondo, M.J.T.: Competition between nitrate and nitrite reduction in denitrification by Pseudomonas Fluorescens. Biotechnol. Bioeng. 46, 476–484 (1995)
APHA.: Standard Methods for the Examination of Water and Wastewater, 19th ed. American Public Health Association, Washington, DC (1995)
Arrojo, B., Mosquera-Corral, A., Garrido, J.M., Mendez, R.: Aerobic granulation with industrial wastewater in sequencing batch reactors. Water Res. 38, 3389–3399 (2004)
Avcioglu, E., Karahan-Gulo, O., Orhon, D.: Estimation of stoichiometric and kinetic coefficients of ASM3 under aerobic and anoxic conditions via respirometry. Water Sci. Technol. 48, 185–194 (2003)
Beun, J.J., Paletta, F., van Loosdrecht, M.C.M., Heijnen, J.J.: Stoichiometry and kinetics of poly-β-hydroxybutyrate metabolism in aerobic, slow growing activated sludge cultures. Biotechnol. Bioeng. 67, 379–389 (2000a)
Beun, J.J., Verhoef, E.V., van Loosdrecht, M.C.M., Heijnen, J.J.: Stoichiometry and kineticskinetics of Poly-β Hydroxybutyrate metabolism under denitrifying conditions in activated sludge culture. Biotechnol. Bioeng. 68, 496–507 (2000b)
Beun, J.J., Heijnen, J.J., van Loosdrecht, M.C.M.: N-removal in a granular sludge sequencing batch airlift reactor. Biotechnol. Bioeng. 75, 82–92 (2001)
Carucci, A., Dionisi, D., Majone, M., Rolle, E., Smurra, P.: Aerobic storage by activated sludge on real wastewater. Water Res. 35, 3833–3844 (2001)
Cokgor, E.U., Sözen, S., Orhon, D., Henze, M.: Respirometric analysis of activated sludge behaviour - I. Assessment of the readily biodegradable substrate. Water Res. 32, 461–475 (1998)
de Kreuk, M.K., van Loosdrecht, M.C.M.: Formation of aerobic granules with domestic sewage. J. Environ. Eng. 132, 694–697 (2006)
Dionisi, D., Majone, M., Ramadori, R., Beccari, M.: The storage of acetate under anoxic conditions. Water Res. 35, 2661–2668 (2001)
Dircks, K., Pind, P.F., Mosbæk, H., Henze, M.: Yield determination by respirometry: The possible influence of storage under aerobic conditions in activated sludge. Water SA 25, 69–74 (1999)
Dircks, K., Henze, M., van Loosdrecht, M.C.M., Mosbaek, H., Aspegren, H.: Storage and degradation of poly-B-hydroxybutyrate in activated sludge under aerobic conditions. Water Res. 35, 2277–2285 (2001)
Flora, E.M.C.V., Suidan, M.T., Flora, J.R.V., Kim, B.J.: Speciation and chemical interactions in nitrifying biofilms II: Sensitivity analysis. J. Environ. Eng-ASCE 125, 878–884 (1999)
Gernaey, K., Petersen, B., Dochain, D., Vanrolleghem, P.A.: Modelling aerobic carbon source degradation processes using titrimetric data and combined respirometric-titrimetric data: structural and practical identifiability. Biotechnol. Bioeng. 79, 754–769 (2002)
Gujer, W., Henze, M., Mino, T., van Loosdrecht, M.C.M.: Activated sludge model NO. 3. Water Sci. Technol. 39, 183–193 (1999)
Hao, X.D., Heijnen, J.J., van Loosdrecht, M.C.M.: Sensitivity analysis of a biofilm model describing a one-stage completely autotrophic nitrogen removal (CANON) process. Biotechnol. Bioeng. 77, 266–277 (2002)
Henze, M., Grady, C.P.L. Jr., Gujer, W., Marais, G.V.R., Matsuo, T.: Activated sludge model No. 1. Scientific and Technical Report No. 1, IAWPRC, London (1987)
Insel, G., Orhon, D., Vanrolleghem, P.A.: Identification and modelling of aerobic hydrolysis mechanism—application of optimal experimental design. J. Chem. Tech. Biotechnol. 78, 437–445 (2003)
Jiang, H.L., Tay, J.H., Maszenan, A.M., Tay, S.T.L.: Enhanced phenol biodegradation and aerobic granulation by two coaggregating bacterial strains. Environ. Sci. Technol. 40, 6137–6142 (2006)
Karahan, O., Artan, N., Orhon, D., Henze, M., van Loosdrecht, M.C.M.: Experimental assessment of bacterial storage yield. J. Environ. Eng. 128, 1030–1035 (2002)
Karahan, O., van Loosdrecht, M.C.M., Orhon, D.: Modification of activated sludge NO. 3 considering direct growth on primary substrate. Water Sci. Technol. 47, 219–225 (2003)
Karahan, O., van Loosdrecht, M.C.M., Orhon, D.: Modeling the utilization of starch by activated sludge for simultaneous substrate storage and microbial growth. Biotechnol. Bioeng. 94, 43–53 (2006a)
Karahan, O., Martins, M., Orhon, D., van Loosdrecht, M.C.M.: Experimental evaluation of starch utilization mechanism by activated sludge. Biotechnol. Bioeng. 93, 964–970 (2006b)
Keesman, K.J., Spanjers, H., van Straten, G.: Analysis of endogenous process behaviour in activated sludge. Biotechnol. Bioeng. 57, 155–163 (1997)
Koch, G., Kuhni, M., Gujer, W., Siegrist, H.: Calibration and validation of activated sludge model no. 3 for Swiss municipal wastewater. Water Res. 34, 3580–3590 (2000)
Krishna, C., van Loosdrecht, M.C.M.: Substrate flux into storage and growth in relation to activated sludge modelling. Water Res. 33, 3149–3161 (1999)
Kristensen, H.G., Elberg Jørgensen, P., Henze, M.: Characterisation of functional micro-organism groups and substrate in activated sludge and wastewater by AUR. NUR and OUR Water Sci. Technol. 25, 43–57 (1992)
Kujawa, K., Klapwijk, B.: A method to estimate denitrification potential for predenitrification systems using NUR batch test. Water Res. 33, 2291–2300 (1999)
Majone, M., Dircks, K., Beun, J.J.: Aerobic storage under dynamic conditions in activated sludge processes––the state of the art. Water Sci. Technol. 39, 61–73 (1999)
Malisse, K.: Monitoring van de denitrificatie in actief slib aan de hand van nitraat - en titrimetrische gegevens. Engineers Thesis. Faculty of Agricultural and Applied Biological Sciences. Ghent University, Dutch (2002)
Naidoo, V., Urbain, V., Buckley, C.A.: Characterisation of wastewater and activated sludge from European municipal wastewater treatment plants using the NUR test. Water Sci. Technol. 38, 303–310 (1998)
Ni, B.J., Yu, H.Q.: Storage and growth of denitrifiers in aerobic granules—Part I: model development. Biotechnol. Bioeng. 99, 314–323 (2008)
Ni, B.J., Yu, H.Q., Xie, W.M.: Storage and growth of denitrifiers in aerobic granules—Part II: model calibration and verification. Biotechnol. Bioeng. 99, 324–332 (2008)
Oh, J., Silverstein, J.: Acetate limitation and nitrite accumulation during denitrification. J. Environ. Eng. 125, 234–242 (1999)
Orhon, D., Söen, S., Artan, N.: The effect of heterotrophic yield on assessment of the correction factor for the anoxic growth. Water Sci. Technol. 34, 67–74 (1996)
Petersen, B.: Calibration, identifiability and optimal experimental design of activated sludge models. Ph.D. Thesis, Ghent University, Belgium (2000)
Petersen, B., Gernaey, K., Vanrolleghem, P.A.: Anoxic activated sludge monitoring with combined nitrate and titrimetric measurements. Water Sci. Technol. 45, 181–190 (2002)
Picioreanu, C., Kreft, J.U., van Loosdrecht, M.C.M.: Particle-based multidimensional multispecies biofilm model. Appl. Environ. Microbiol. 70, 3024–3040 (2004)
Pratt, S., Yuan, Z., Keller, J.: Modelling aerobic carbon oxidation and storage by integrating respirometric, titrimetric, and off-gas CO2 measurements. Biotechnol. Bioeng. 88, 135–147 (2004)
Reichert, P.: AQUASIM 2.0—User Manual, Computer Program for the Identification and Simulation of Aquatic Systems. Swiss Federal Institute for Environmental Science and Technology (EAWAG) (1998)
Schwarzenbeck, N., Erley, R., McSwain, B.S., Wilderer, P.A., Irvine, R.L.: Treatment of malting wastewater in a granular sludge sequencing batch reactor (SBRSBR). Acta Hydrochim. Hydrobiol. 32, 16–24 (2004)
Shahalam, A.B., Elsamra, R., Ayoub, G.M., Acra, A.: Parametric sensitivity of comprehensive model of aerobic fluidized-bed biofilm process. J Environ. Eng-ASCE 122, 1085–1093 (1996)
Siegrist, H., Vogt, D., Garcia-Heras, J.L., Gujer, W.: Mathematical model for meso- and thermophilic anaerobic sewage sludge digestion. Environ. Sci. Technol. 36, 1113–1123 (2002)
Sin, G.: Systematic calibration of activated sludge model. Thesis for the Degree of Doctor (Ph.D) in Applied Biological Sciences: Environmental Technology. Ghent University (2004)
Sin, G., Guisasola, A., De Pauw, D.J.W., Baeza, J.A., Carrera, J., Vanrolleghem, P.A.: A new approach for modelling simultaneous storage and growth processes for activated sludge systems under aerobic conditions. Biotechnol. Bioeng. 92, 600–613 (2005)
Soen, A., Ubay, Cokgor E., Henze, Orhon D., M., : Respirometric analysis of activated sludge behaviour––II. Heterotrophic growth under aerobic and anoxic conditions. Water Sci. Technol. 32, 476–488 (1998)
Sozen, S., Orhon, D.: The effect of nitrite correction on the evaluation of the rate of nitrate utilization under anoxic conditions. J. Chem. Technol. Biotechnol. 74, 790–800 (1999)
Su, K.Z., Yu, H.Q.: Formation and characterization of aerobic granules in a sequencing batch reactor treating soybean processing wastewater. Environ. Sci. Technol. 39, 2818–2827 (2005)
Su, K.Z., Yu, H.Q.: A generalized model for aerobic granule-based sequencing batch reactor 1. Model development. Environ. Sci. Technol. 40, 4703–4708 (2006)
Tay, S.T.L., Zhang, W.Q., Tay, J.H.: Start-up, microbial community analysis and formation of aerobic granules in a tert-butyl alcohol degrading sequencing batch reactor. Environ. Sci. Technol. 39, 5774–5780 (2005)
Thomsen, J.K., Geest, T., Cox, R.P.: Mass spectrometric studies of the effect of pH on the accumulation of intermediates in denitrification by Paracoccus denitrificans. Appl. Environ. Microbiol. 60, 536–541 (1994)
van Loosdrecht, M.C.M., Heijnen, J.J.: Modelling of activated sludge processes with structured biomass. Water Sci. Technol. 45, 12–23 (2002)
van Loosdrecht, M.C.M., Henze, M.: Maintenance, endogeneous respiration, lysis, decay and predation. Water Sci. Technol. 39, 107–117 (1999)
van Loosdrecht, M.C.M., Pot, M.A., Heijnen, J.J.: Importance of bacterial storage polymers in bioprocesses. Water Sci. Technol. 35, 41–47 (1997)
Vanrolleghem, P.A., Van Daele, M., Dochain, D.: Practical identifiability of a biokinetic model of activated sludge respiration. Water Res. 29, 2561–2570 (1995)
Vanrolleghem, P.A., Spanjers, H., Petersen, B., Ginestet, P., Takács, I.: Estimating (combinations of) activated sludge model No.1 parameters and components by respirometry. Water Sci. Technol. 39, 195–215 (1999)
Vanrolleghem, P., Sin, G., Gernaey, K.: Transient response of aerobic and anoxic activated sludge activities to sudden concentration changes. Biotechnol. Bioeng. 86, 277–290 (2004)
Wanner, O., Reichert, P.: Mathematical modelling of mixed-culture bioflms. Biotechnol. Bioeng. 49, 172–184 (1996)
Wild, D., Kisliakova, A., Siegrist, H.: P-fixation by Mg, Ca and zeolite A during stabilization of excess sludge from enhanced biological P-removal. Water Sci. Technol. 34, 391–398 (1996)
Wilderer, P.A., Warren, L.J., Dau, U.: Competition in denitrification systems affecting reduction rate and accumulation of nitrite. Water Res. 21, 239–245 (1987)
Xu, S.L., Hultman, B.: Experiences in wastewater characterization and model calibration for the activated sludge process. Water Sci. Technol. 33, 89–98 (1996)
Yi, S., Zhang, W.Q., Wu, B., Tay, S.T.L., Tay, J.H.: Biodegradation of p-nitrophenol by aerobic granules in a sequencing batch reactor. Environ. Sci. Technol. 40, 2396–2401 (2006)
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Ni, BJ. (2013). Storage and Growth Processes in Aerobic Granular Sludge. In: Formation, characterization and mathematical modeling of the aerobic granular sludge. Springer Theses, vol 131. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-31281-6_6
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