Allelopathy pp 407-427 | Cite as

Microscopy for Modeling of Cell–Cell Allelopathic Interactions

  • Victoria V. Roshchina
  • V. A. Yashin
  • Alexandra V. Yashina
  • M. V. Goltyaev


The application of various microscopy methods—from stereomicroscopy to luminescence microscopy, microspectrofluorimetry and laser-scanning confocal microscopy—has been considered as an approach to model the cell–cell contacts and interactions in allelopathy. It bases on the direct observations of both secretions released from allelopathic species and the interaction(s) with the cell acceptors as biosensors (unicellular plant generative and vegetative microspores). Special attention was paid to the interactions with pigmented and fluorescing components of the secretions released by the cell donors from allelopathically active plant species. Colored allelochemicals are considered as histochemical dyes for the analysis of cellular mechanisms at the allelopathic contacts.


Fluorescence Spectrum Donor Cell Pollen Germination Allelopathic Interaction Optical Slice 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


  1. Aliotta G, Cafiero G (1999) Biological properties of rue (Ruta graveolens L.). Potential use in sustainable agricultural systems. In: Dakshini KMM (Inderjit), Foy CL (eds) Principles and practices in plant ecology: allelochemical interactions. CRC Press, Boca Raton, pp 551–563Google Scholar
  2. Alstyne KL, Nelson AV, Vyvyan JR, Cancilla DA (2006) Dopamine functions as an antiherbivore defense in the temperate green alga Ulvaria obscura. Oecologia 148:304–311PubMedCrossRefGoogle Scholar
  3. Gaur S, Rana A, Chauhan SVS (2007) Pollen allelopathy: past achievements and future approach. Allelopathy J 20:115–126Google Scholar
  4. Gerbach PV (2002) The essential oil secreting structures of Prostanthera ovalifolia (Lamiaceae). Ann Bot 89:255–260CrossRefGoogle Scholar
  5. Gilroy S (1997) Fluorescence microscopy of living plant cells. Annu Rev Plant Physiol Plant Mol Biol 48:165–190PubMedCrossRefGoogle Scholar
  6. Golovkin BN, Rudenskaya RN, Trofimova IA, Shreter AI (2001) Biologically active substances of plant origin, vol 3. Nauka, MoscowGoogle Scholar
  7. Hazak O, Bloch D, Poraty L, Stemberg H, Zhang J, Friml J, Yalovsky S (2010) A rho scaffold integrates the secretory system with feedback mechanisms in regulation of auxin distribution. PLoS Biol 8(1):e1000282. doi: 10.1371/journal.pbio.1000282 PubMedCrossRefGoogle Scholar
  8. Huang X, Jiang H, Hao G (2009) Direct HPLC detection of benzodilactones and quinones in glands of Lysimachia fordiana. Fitoterapia 80:173–176PubMedCrossRefGoogle Scholar
  9. Jaldappagari S, Motohashi N, Gangeenahalli MP, Naismith JH (2008) Bioactive mechanism of interaction between anthocyanins and macromolecules like DNA and proteins. Topics Heterocycl Chem 15:49–65CrossRefGoogle Scholar
  10. Karnaukhov VN, Yashin VA, Kulakov VI, Vershinin VM, Dudarev VV (1982) Apparatus for investigation of fluorescence characteristics of microscopic objects. US Patent, N4, 354, 114:1–14Google Scholar
  11. Karnaukhov VN, Yashin VA, Kulakov VI, Vershinin VM, Dudarev VV (1983) Apparatus for investigation of fluorescence characteristics of microscopic objects. Patent of England 2.039.03 R5R.CHIGoogle Scholar
  12. Karnaukhov VN, Yashin VA, Kazantsev AP, Karnaukhova NA, Kulakov VI (1987) Double-wave microfluorimeter-photometer based on standard attachment. Tsitologia (Cytology, USSR) 29:113–116Google Scholar
  13. Karnaukhova NA, Sergievich LA, Karnaukhov VN (2010) Application of microspectral analysis to study intracellular metabolism in single cells and cell systems. Nat Sci 2:444–449Google Scholar
  14. Mathesius U, Bayliss C, Weinman JJ, Schlaman HRM, Spaink HP, Rolfe BG, McCully ME, Djordjevic MA (1998) Flavonoids synthesized in cortical cells during nodule initiation are early developmental markers in white clover. Molec Plant Microbe Interact 11(12):1223–1232CrossRefGoogle Scholar
  15. Murphy SD (1992) The determination of allelopathic potential of pollen and nectar. In: Linskens HF, Jackson IF (eds) Plant toxins analysis. Springer, Berlin, pp 333–357Google Scholar
  16. Murphy SD (1999) Pollen allelopathy. In: Dakshini KMM (Inderjit), Foy CL (eds) Principles and practices in plant ecology: allelochemical interactions. CRC Press, Boca Raton, pp 129–148Google Scholar
  17. Murphy SD (2007) Allelopathic pollen: isolating the allelopathic effects. In: Roshchina VV, Narwal SS (eds) Cell diagnostics. Science Publisher, Enfield, pp 185–198Google Scholar
  18. Pacek A, Stpiczynska M (2007) The structures of elaiophores of Oncidium cheirophorum Rchb.F. and Ornithocephalus kruegeri Rchb.F. (Orchidaceae). Acta Agrobot 60:9–14Google Scholar
  19. Pawley J, Pawley JB (2006) Handbook of biological confocal microscopy. Springer, BerlinCrossRefGoogle Scholar
  20. Roshchina VV (1999) Mechanisms of cell–cell communication. In: Narwal SS (ed) Allelopathy update, vol 2. Science Publishers, Enfield, pp 3–25Google Scholar
  21. Roshchina VV (2001a) Neurotransmitters in plant life. Science Publisher, EnfieldGoogle Scholar
  22. Roshchina VV (2001b) Molecular-cellular mechanisms in pollen alllelopathy. Allelopathy J 8:11–28Google Scholar
  23. Roshchina VV (2002) Rutacridone as a fluorescent dye for the study of pollen. J Fluoresc 12:241–243CrossRefGoogle Scholar
  24. Roshchina VV (2003) Autofluorescence of plant secreting cells as a biosensor and bioindicator reaction. J Fluoresc 13:403–420CrossRefGoogle Scholar
  25. Roshchina VV (2004) Cellular models to study the allelopathic mechanisms. Allelopathy J 13:3–16Google Scholar
  26. Roshchina VV (2005) Allelochemicals as fluorescent markers, dyes and probes. Allelopathy J 16:31–46Google Scholar
  27. Roshchina VV (2006a) Plant microspores as biosensors. Trends Modern Biol 126:262–274Google Scholar
  28. Roshchina VV (2006b) Chemosignaling in plant microspore cells. Biol Bull 33:414–420Google Scholar
  29. Roshchina VV (2007a) Cellular models as biosensors. In: Roshchina VV, Narwal SS (eds) Cell diagnostics. Science Publisher, Enfield, pp 5–22Google Scholar
  30. Roshchina VV (2007b) Luminescent cell analysis in allelopathy. In: Roshchina VV, Narwal SS (eds) Cell diagnostics. Science Publisher, Enfield, pp 103–115Google Scholar
  31. Roshchina VV (2008) Fluorescing world of plant secreting cells. Science Publisher, EnfieldGoogle Scholar
  32. Roshchina VV, Karnaukhov VN (2010) The fluorescence analysis of the medicinal drugs’ interaction with unicellular biosensors. Pharmacia (Russia) 3:43–46Google Scholar
  33. Roshchina VV, Melnikova EV (1995) Spectral analysis of intact secretory cells and excretions of plants. Allelopathy J 2:179–188Google Scholar
  34. Roshchina VV, Melnikova EV (1996) Microspectrofluorometry: a new technique to study pollen allelopathy. Allelopathy J 3:51–58Google Scholar
  35. Roshchina VV, Melnikova EV (1999) Microspectrofluorimetry of intact secreting cells, with applications to the study of allelopathy. In: Dakshini KMM (Inderjit), Foy CL (eds) Principles and practices in plant ecology: allelochemical interactions. CRC Press, Boca Raton, pp 99–126Google Scholar
  36. Roshchina VV, Melnikova EV (1998) Allelopathy and plant generative cells. Participation of acetylcholine and histamine in a signalling at the interactions of pollen and pistil. Allelopathy J 5:171–182Google Scholar
  37. Roshchina VV, Roshchina VD (1993) The excretory function of higher plants. Springer, BerlinCrossRefGoogle Scholar
  38. Roshchina VV, Melnikova EV, Spiridonov NA, Kovaleva LV (1995) Azulenes, the blue pigments of pollen. Doklady Biol Sci 340:93–96Google Scholar
  39. Roshchina VV, Melnikova EV, Kovaleva LV (1996) Autofluorescence in system pollen-pistil of Hippeastrum hybridum. Doklady Biol Sci 349:118–120Google Scholar
  40. Roshchina VV, Melnikova EV, Karnaukhov VN, Golovkin BN (1997) Application of microspectrofluorimetry in spectral analysis of plant secretory cells. Biol Bull (Russia) 2:167–171Google Scholar
  41. Roshchina VV, Melnikova EV, Mit’kovskaya LI, Karnaukhov VN (1998) Microspectrofluorimetry for the study of intact plant secretory cells. J Gen Biol (Russia) 59:531–554Google Scholar
  42. Roshchina VV, Melnikova EV, Yashin VA, Karnaukhov VN (2002) Autofluorescence of intact spores of horsetail Equisetum arvense L. during their development. Biophysics (Russia) 47:318–324Google Scholar
  43. Roshchina VV, Yashin VA, Kononov AV (2004) Autofluorescence of plant microspores studied by confocal microscopy and microspectrofluorimetry. J Fluoresc 14:745–750PubMedCrossRefGoogle Scholar
  44. Roshchina VV, Yashin VA, Kononov AV, Yashina AV (2007) Laser-scanning confocal microscopy (LSCM): study of plant secretory cells. In: Roshchina VV, Narwal SS (eds) Cell diagnostics. Science Publisher, Enfield, pp 93–102Google Scholar
  45. Roshchina VV, Yashina AV, Yashin VA (2008) Cell communication in pollen allelopathy analyzed with laser-scanning confocal microscopy. Allelopathy J 21:219–226Google Scholar
  46. Roshchina VV, Yashina AV, Yashin VA, Prizova NK (2009a) Models to study pollen allelopathy. Allelopathy J 23:3–24Google Scholar
  47. Roshchina VV, Yashin VA, Yashina AV, Gol’tyaev MV, Manokhina IA (2009b) Microscopic objects for the study of chemosignaling. In: Zinchenko VP, Kolesnikov SS, Berezhnov AV (eds) Reception and intracellular signalling. Biological Center of RAS, Pushchino, pp 699–703Google Scholar
  48. Roshchina VV, Yashina AV, Yashin VA, Gol’tyaev MV (2011a) Fluorescence of biologically active compounds in plant secretory cells. In: Narwal SS, Pavlovic P, Jacob J (eds) Research methods in plant science, vol 2., Forestry and AgroforestryStudium Press, Houston, pp 3–25Google Scholar
  49. Roshchina VV, Yashin VA, Yashina AV, Gol’tyaev MV (2011b) Colored allelochemicals in modelling of cell–cell allelopathic interactions. Allelopathy J 28:1–12Google Scholar
  50. Roshchina VV, Yashin VA, Vikhlyantsev IM (2011c) Fluorescence of plant microspores as biosensors. Biol Membr 28:1–12CrossRefGoogle Scholar
  51. Roy S, Bhattacharya S, Das P, Chattopadhyay J (2007) Interaction among non-toxic phytoplankton, toxic phytoplankton and zooplankton: inferences from field observations. J Biol Phys 33:1–17PubMedCrossRefGoogle Scholar
  52. Salih A, Jones A, Bass D, Cox G (1997) Confocal imaging of exine for grass pollen analysis. Grana 36:215–224CrossRefGoogle Scholar
  53. Sharma AD, Sharma R (1999) Anthocyanin-DNA copigmentation complex: mutual protection against oxidative damage. Phytochem 52:1313–1318CrossRefGoogle Scholar
  54. Solé J, García-Ladona E, Ruardij P, Estrada M (2005) Modelling allelopathy among marine alga. Ecol Model 183:373–384CrossRefGoogle Scholar
  55. Stanley RG, Linskens HF (1974) Pollen, biology, biochemistry, managements. Springer, BerlinGoogle Scholar
  56. Wymer CL, Beven AF, Boudonck K, Lloyd CW (1999) Confocal microscopy of plant cells. Methods Molec Biol 122:103–130Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Victoria V. Roshchina
    • 1
  • V. A. Yashin
    • 1
  • Alexandra V. Yashina
    • 1
  • M. V. Goltyaev
    • 1
  1. 1.Institute of Cell Biophysics, RASPushchinoRussia

Personalised recommendations