Preimplantation Mouse Embryo: Developmental Fate and Potency of Blastomeres

  • Aneta Suwińska
Part of the Results and Problems in Cell Differentiation book series (RESULTS, volume 55)


During the past decade we have witnessed great progress in the understanding of cellular, molecular, and epigenetic aspects of preimplantation mouse development. However, some of the issues, especially those regarding the nature and regulation of mouse development, are still unresolved and controversial and raise heated discussion among mammalian embryologists. This chapter presents different standpoints and various research approaches aimed at examining the fate and potency of cells (blastomeres) of mouse preimplantation embryo. In dealing with this subject, it is important to recognize the difference between the fate of blastomere and the prospective potency of blastomere, with the first being its contribution to distinct tissues during normal development, and the second being a full range of its developmental capabilities, which can be unveiled only by experimental perturbation of the embryo. Studies of the developmental potential and the fate of blastomeres are of the utmost importance as they may lead to future clinical application in reproductive and regenerative medicine.


Zona Pellucida Preimplantation Genetic Diagnosis Blastocyst Stage Inner Cell Mass Cdx2 Expression 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



I would like to dedicate this work to Professor A.K. Tarkowski, my mentor, as an expression of respect for his work and scientific achievements. I am grateful to Professor A.K. Tarkowski, Professor M. Kloc, Dr. J. Kubiak, and Professor M. Maleszewski for critical reading and valuable suggestions. During the preparation of this work Aneta Suwinska was supported by the grant in the framework of PARENT-BRIDGE Programme from the Foundation for Polish Science POMOST/2010-1/9 and a grant from Polish Ministry of Science and Higher Education N N301 311637.


  1. Alarcón VB, Marikawa Y (2003) Deviation of the blastocyst axis from the first cleavage plane does not affect the quality of mouse postimplantation development. Biol Reprod 69:1208–1212PubMedGoogle Scholar
  2. Alarcón VB, Marikawa Y (2005) Unbiased contribution of the first two blastomeres to mouse blastocyst development. Mol Reprod Dev 72:354–361PubMedGoogle Scholar
  3. Alarcón VB, Marikawa Y (2008) Spatial alignment of the mouse blastocyst axis across the first cleavage plane is caused by mechanical constraint rather than developmental bias among blastomeres. Mol Reprod Dev 75:1143–1153PubMedGoogle Scholar
  4. Chazaud C, Yamanaka Y, Pawson T, Rossant J (2006) Early lineage segregation between epiblast and primitive endoderm in mouse blastocysts through the Grb2-MAPK pathway. Dev Cell 10:615–624PubMedGoogle Scholar
  5. Chroscicka A, Komorowski S, Maleszewski M (2004) Both blastomeres of the mouse 2-cell embryo contribute to the embryonic portion of the blastocyst. Mol Reprod Dev 68:308–312PubMedGoogle Scholar
  6. Chung Y, Klimanskaya I, Becker S, Marh J, Lu SJ, Johnson J, Meisner L, Lanza R (2005) Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres. Nature 439:216–219PubMedGoogle Scholar
  7. Davies TJ, Gardner RL (2002) The plane of first cleavage is not related to the distribution of sperm components in the mouse. Hum Reprod 17:2368–2379PubMedGoogle Scholar
  8. Dietrich JE, Hiiragi T (2007) Stochastic patterning in the mouse pre-implantation embryo. Development 134:4219–4231PubMedGoogle Scholar
  9. Dziadek M (1979) Cell differentiation in isolated inner cell masses of mouse blastocysts in vitro: onset of specific gene expression. J Embryol Exp Morphol 53:367–379PubMedGoogle Scholar
  10. Fleming TP, Warren PD, Chisholm JC, Johnson MH (1984) Trophectodermal processes regulate the expression of totipotency within the inner cell mass of the mouse expanding blastocyst. J Embryol Exp Morphol 84:63–90PubMedGoogle Scholar
  11. Fujimori T, Kurotaki Y, Miyazaki J, Nabeshima Y (2003) Analysis of cell lineage in two- and four-cell mouse embryos. Development 130:5113–5122PubMedGoogle Scholar
  12. Gardner RL (1985) Regeneration of endoderm from primitive ectoderm in the mouse embryo: fact or artifact. J Embryol Exp Morphol 88:303–326PubMedGoogle Scholar
  13. Gardner RL (1997) The early blastocyst is bilaterally symmetrical and its axis of symmetry is aligned with the animal-vegetal axis of the zygote in the mouse. Development 124:289–301PubMedGoogle Scholar
  14. Gardner RL (2001) Specification of embryonic axes begins before cleavage in normal mouse development. Development 128:839–847PubMedGoogle Scholar
  15. Gardner RL, Davies TJ (2003) Is the plane of first cleavage related to the point of sperm entry in the mouse? Reprod Biomed Online 6:157–160PubMedGoogle Scholar
  16. Gardner RL, Johnson MH (1972) An investigation of inner cell mass and trophoblast tissues following their isolation from the mouse blastocyst. J Embryol Exp Morphol 28:279–312PubMedGoogle Scholar
  17. Gardner R, Rossant J (1979) Investigation of the fate of 4,5 day post-coitum mouse inner cell mass by blastocyst injection. J Embryol Exp Morphol 52:141–152PubMedGoogle Scholar
  18. Geens M, Mateizel I, Sermon K, De Rycke M, Spits C, Cauffman G, Devroey P, Tournaye H, Liebaers I, Van de Velde H (2009) Human embryonic stem cell lines derived from single blastomeres of two 4-cell stage embryos. Hum Reprod 24:2709–2717PubMedGoogle Scholar
  19. González S, Ibáñez E, Santaló J (2011) Influence of early fate decisions at the two-cell stage on the derivation of mouse embryonic stem cell lines. Stem Cell Res 7:54–65PubMedGoogle Scholar
  20. Gray D, Plusa B, Piotrowska K, Na J, Tom B, Glover DM, Zernicka-Goetz M (2004) First cleavage of the mouse embryo responds to change in egg shape at fertilization. Curr Biol 14:397–405PubMedGoogle Scholar
  21. Guo G, Huss M, Tong GQ, Wang C, Sun LL, Clarke ND, Robson P (2010) Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell 18:675–685Google Scholar
  22. Handyside AH (1978) Time of commitment of inside cells isolated from preimplantation mouse embryos. J Embryol Exp Morphol 45:37–53PubMedGoogle Scholar
  23. Handyside AH, Barton SC (1977) Evaluation of the technique of immunosurgery for the isolation of inner cell masses from mouse blastocysts. J Embryol Exp Morphol 37:217–226PubMedGoogle Scholar
  24. Hansis C, Grifo JA, Tang Y, Krey LC (2002) Assessment of beta-HCG, beta-LH mRNA and ploidy in individual human blastomeres. Reprod Biomed Online 5:156–161PubMedGoogle Scholar
  25. Hansis C, Grifo JA, Krey LC (2004) Candidate lineage marker genes in human preimplantation embryos. Reprod Biomed Online 8:577–583PubMedGoogle Scholar
  26. Hardy K, Martin KL, Leese HJ, Winston RM, Handyside AH (1990) Human preimplantation development in vitro is not adversely affected by biopsy at the 8-cell stage. Hum Reprod 5:708–714PubMedGoogle Scholar
  27. Hiiragi T, Solter D (2004) First cleavage plane of the mouse egg is not predetermined but defined by the topology of the two apposing pronuclei. Nature 430:360–364PubMedGoogle Scholar
  28. Hiiragi T, Alarcon VB, Fujimori T, Louvet-Vallee S, Maleszewski M, Marikawa Y, Maro B, Solter D (2006) Where do we stand now? Mouse early embryo patterning meeting in Freiburg, Germany. Int J Dev Biol 50:581–586PubMedGoogle Scholar
  29. Hogan B, Tilly R (1978a) In vitro development of inner cell masses isolated immunosurgically from mouse blastocysts. II. Inner cell masses from 3,5- to 4,0-day p.c. blastocysts. J Embryol Exp Morphol 45:107–21PubMedGoogle Scholar
  30. Hogan B, Tilly R (1978b) In vitro development of inner cell masses isolated immunosurgically from mouse blastocysts. I. Inner cell masses from 3,5-day p.c. blastocysts incubated for 24h before immunosurgery. J Embryol Exp Morphol 45:93–105PubMedGoogle Scholar
  31. Handyside AH, Kontogianni EH, Hardy K, Winston RM (1990) Pregnancies from biopsied human preimplantation embryos sexed by Y-specific DNA amplification. Nature 344:768–770PubMedGoogle Scholar
  32. James RM, Klerkx AHEM, Keighren M, Flockhart JH, West JD (1995) Restricted distribution of tetraploid cells in mouse tetraploid <==> diploid chimaeras. Dev Biol 167:213–226PubMedGoogle Scholar
  33. Jedrusik A, Parfitt DE, Guo G, Skamagki M, Grabarek JB, Johnson MH, Robson P, Zernicka-Goetz M (2008) Role of Cdx2 and cell polarity in cell allocation and specification of trophectoderm and inner cell mass in the mouse embryo. Genes Dev 22:2692–2706PubMedGoogle Scholar
  34. Johnson MH, Ziomek CA (1981) The foundation of two distinct cell lineages within the mouse morula. Cell 24:71–80PubMedGoogle Scholar
  35. Johnson WH, Loskutoff NM, Plante Y, Betteridge KJ (1995) Production of four identical calves by the separation of blastomeres from an in vitro derived four-cell embryo. Vet Rec 137:15–16PubMedGoogle Scholar
  36. Kelly SJ (1975) Studies of the potency of the early cleavage blastomeres of the mouse. In: Balls M, Wild AE (eds) The early development of mammals. Cambridge University Press, Cambridge, pp 97–105Google Scholar
  37. Kelly SJ (1977) Studies of the developmental potential of 4- and 8-cell stage mouse blastomeres. J Exp Zool 200:365–376PubMedGoogle Scholar
  38. Klimanskaya I, Chung Y, Becker S, Lu SJ, Lanza R (2007) Derivation of human embryonic stem cells from single blastomeres. Nat Protoc 2:1963–1972PubMedGoogle Scholar
  39. Kubiak JZ, Tarkowski AK (1985) Electrofusion of mouse blastomeres. Exp Cell Res 157:561–566PubMedGoogle Scholar
  40. Kurimoto K, Kabuta Y, Ohinata Y, Ono Y, Uno KD, Hamada RG, Ueda HR, Saitou M (2006) An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis. Nucleic Acids Res 34:e42PubMedGoogle Scholar
  41. Kurotaki Y, Hatta K, Nakao K, Nabeshima Y, Fujimori T (2007) Blastocyst axis is specified independently of early cell lineage but aligns with the ZP shape. Science 316:719–723PubMedGoogle Scholar
  42. Louvet-Vallee S, Dard N, Santa-Maria A, Aghion J, Maro B (2001) A major posttranslational modification of ezrin takes place during epithelial differentiation in the early mouse embryo. Dev Biol 231:190–200PubMedGoogle Scholar
  43. Louvet-Vallée S, Vinot S, Maro B (2005) Mitotic spindles and cleavage planes are oriented randomly in the two-cell mouse embryo. Curr Biol 15:464–469PubMedGoogle Scholar
  44. Markert CL, Petters RM (1978) Manufactured hexaparental mice show that adults are derived from three embryonic cells. Science 202:56–58PubMedGoogle Scholar
  45. MacKay GE, West JD (2005) Fate of tetraploid cells in 4n↔2n chimeric mouse blastocysts. Mech Dev 122:1266–1281PubMedGoogle Scholar
  46. McGrath J, Solter D (1984) Completion of mouse embryogenesis requires both the maternal and paternal genomes. Cell 37:179–183PubMedGoogle Scholar
  47. McLaren A (1976) Mammalian chimaeras. Cambridge University Press, Cambridge, London, New York, MelbourneGoogle Scholar
  48. Mintz B (1962) Formation of genotypically mosaic mouse embryos. Am Zool 2:432Google Scholar
  49. Mintz B (1964) Formation of genetically mosaic mouse embryos and early development of “lethal (t 12/t 12)-normal” mosaics. J Exp Zool 157:273–292PubMedGoogle Scholar
  50. Meilhac SM, Adams RJ, Morris SA, Danckaert A, Le-Garrec JF, Zernicka-Goetz M (2009) Active cell movements coupled to positional induction are involved in lineage segregation in the mouse. Dev Biol 331:210–221PubMedGoogle Scholar
  51. Moore NW, Adams CE, Rowson LEA (1968) Developmental potential of single blastomeres of the rabbit egg. J Reprod Fertil 17:527–531PubMedGoogle Scholar
  52. Morris SA, Teo RT, Li H, Robson P, Glover DM, Zernicka-Goetz M (2010) Origin and formation of the first two distinct cell types of the inner cell mass in the mouse embryo. Proc Natl Acad Sci USA 107:6364–6369PubMedGoogle Scholar
  53. Motosugi N, Bauer T, Polanski Z, Solter D, Hiiragi T (2005) Polarity of the mouse embryo is established at blastocyst and is not prepatterned. Genes Dev 19:1081–1092PubMedGoogle Scholar
  54. Mullen RJ, Whitten WK, Carter SC (1970) Studies on chimeric mice and half-embryos. In: Annual report of the Jackson Laboratory. Bar harbor, Maine, pp 67–68Google Scholar
  55. Nichols J, Gardner RL (1984) Heterogeneous differentiation of external cells in individual isolated early mouse inner cell masses in culture. J Embryol Exp Morphol 80:225–240PubMedGoogle Scholar
  56. Ozdzeński W, Szczesny E, Tarkowski AK (1997) Postimplantation development of mouse blastocysts with two separate inner cell masses. Anat Embryol (Berl) 195:467–471Google Scholar
  57. Pedersen RA, Spindle AI, Wiley LM (1977) Regeneration of endoderm by ectoderm isolated from mouse blastocysts. Nature 270:453–457Google Scholar
  58. Pierce GB, Arechaga J, Muro C, Wells RS (1988) Differentiation of ICM cells into trophectoderm. Am J Pathol 132:356–364PubMedGoogle Scholar
  59. Pinyopummin A, Takahashi Y, Hishinuma M, Kanagawa H (1994) Development of single blastomeres from 4-cell stage embryos after aggregation with parthenogenones in mice. Jpn J Vet Res 42:119–126PubMedGoogle Scholar
  60. Piotrowska K, Zernicka-Goetz M (2001) Role for sperm in spatial patterning of the early mouse embryo. Nature 409:517–521PubMedGoogle Scholar
  61. Piotrowska K, Wianny F, Pedersen RA, Zernicka-Goetz M (2001) Blastomeres arising from the first cleavage division have distinguishable fates in normal mouse development. Development 128:3739–3748PubMedGoogle Scholar
  62. Piotrowska-Nitsche K, Perea-Gomez A, Haraguchi S, Zernicka-Goetz M (2005) Four-cell stage mouse blastomeres have different developmental properties. Development 132:479–490PubMedGoogle Scholar
  63. Plachta N, Bollenbach T, Pease S, Fraser SE, Pantazis P (2011) Oct4 kinetics predict cell lineage patterning in the early mammalian embryo. Nat Cell Biol 13:117–123PubMedGoogle Scholar
  64. Plusa B, Grabarek JB, Piotrowska K, Glover DM, Zernicka-Goetz M (2002a) Site of the previous meiotic division defines cleavage orientation in the mouse embryo. Nat Cell Biol 4:811–815PubMedGoogle Scholar
  65. Plusa B, Piotrowska K, Zernicka-Goetz M (2002b) Sperm entry position provides a surface marker for the first cleavage plane of the mouse zygote. Genesis 32:193–198PubMedGoogle Scholar
  66. Plusa B, Hadjantonakis AK, Gray D, Piotrowska-Nitsche K, Jedrusik A, Papaioannou VE, Glover DM, Zernicka-Goetz M (2005) The first cleavage of the mouse zygote predicts the blastocyst axis. Nature 434:391–395PubMedGoogle Scholar
  67. Plusa B, Piliszek A, Frankenberg S, Artus J, Hadjantonakis AK (2008) Distinct sequential cell behaviours direct primitive endoderm formation in the mouse blastocyst. Development 135:3081–3091PubMedGoogle Scholar
  68. Ralston A, Rossant J (2008) Cdx2 acts downstream of cell polarization to cell-autonomously promote trophectoderm fate in the early mouse embryo. Dev Biol 313:614–629PubMedGoogle Scholar
  69. Randle BJ (1982) Cosegregation of monoclonal antibody reactivity and cell behaviour in the mouse preimplantation embryo. J Embryol Exp Morphol 70:261–278PubMedGoogle Scholar
  70. Rossant J (1975a) Investigation of the determinative state of the mouse inner cell mass. II. The fate of isolated inner cell masses transferred to the oviduct. J Embryol Exp Morphol 33:991–1001PubMedGoogle Scholar
  71. Rossant J (1975b) Investigation of the determinative state of the mouse inner cell mass. I. Aggregation of isolated inner cell masses with morulae. J Embryol Exp Morphol 33:979–990PubMedGoogle Scholar
  72. Rossant J (1976) Postimplantation development of blastomeres isolated from 4- and 8-cell mouse eggs. J Embryol Exp Morphol 36:283–290PubMedGoogle Scholar
  73. Rossant J, Lis WT (1979) Potential of isolated mouse inner cell masses to form trophectoderm derivatives in vivo. Dev Biol 70:255–261PubMedGoogle Scholar
  74. Rossant J, Vijh KM (1980) Ability of outside cells from preimplantation mouse embryos to form inner cell mass derivatives. Dev Biol 76:475–482PubMedGoogle Scholar
  75. Seidel F (1952) Die entwicklugspotenzen einen isolierten blastomere des zweizellen-stadiums im saugetierei. Naturwissenschaften 39:355–356Google Scholar
  76. Seidel F (1960) Die entwicklungsfahigkeiten isolierter furchungszellen aus dem ei des kaninchens Oryctolagus cuniculus. Roux Arch Entw Mech 152:43–130Google Scholar
  77. Solter D, Knowles BB (1975) Immunosurgery of mouse blastocyst. Proc Natl Acad Sci USA 72:5099–5102PubMedGoogle Scholar
  78. Spindle AI (1978) Trophoblast regeneration by inner cell masses isolated from cultured mouse embryos. J Exp Zool 203:483–489PubMedGoogle Scholar
  79. Staessen C, Platteau P, Van Assche E, Michiels A, Tournaye H, Camus M, Devroey P, Liebaers I, Van Steirteghem A (2004) Comparison of blastocyst transfer with or without preimplantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: a prospective randomized controlled trial. Hum Reprod 19:2849–2858PubMedGoogle Scholar
  80. Strumpf D, Mao CA, Yamanaka Y, Ralston A, Chawengsaksophak K, Beck F, Rossant J (2005) Cdx2 is required for correct cell fate specification and differentiation of trophectoderm in the mouse blastocyst. Development 132:2093–2102PubMedGoogle Scholar
  81. Surani MAH, Handyside AH (1983) Reassortment of cells according to position in mouse morulae. J Exp Zool 225:505–511PubMedGoogle Scholar
  82. Surani MA, Barton SC, Norris ML (1984) Development of reconstituted mouse eggs suggests imprinting of the genome during gametogenesis. Nature 308:548–550PubMedGoogle Scholar
  83. Suwinska A, Czolowska R, Ozdzenski W, Tarkowski AK (2008) Blastomeres of the mouse embryo lose totipotency after the fifth cleavage division: expression of Cdx2 and Oct4 and developmental potential of inner and outer blastomeres of 16- and 32-cell embryos. Dev Biol 322:133–44PubMedGoogle Scholar
  84. Szczepanska K, Stanczuk L, Maleszewski M (2011) Isolated mouse inner cell mass is unable to reconstruct trophectoderm. Differentiation 82:1–8PubMedGoogle Scholar
  85. Tarkowski AK (1959a) Experiments on the development of isolated blastomeres of mouse eggs. Nature 184:1286–1287PubMedGoogle Scholar
  86. Tarkowski AK (1959b) Experimental studies on regulation in the development of isolated blastomeres of mouse eggs. Acta Theriol 3:191–267Google Scholar
  87. Tarkowski AK (1961) Mouse chimaeras developed from fused eggs. Nature 190:857–860PubMedGoogle Scholar
  88. Tarkowski AK (1998) Mouse chimaeras revisited: recollections and reflections. Int J Dev Biol 42:903–908PubMedGoogle Scholar
  89. Tarkowski AK (1963) Studies on mouse chimeras developed from eggs fused in vitro. Natl Cancer Inst Monogr 11:51–71PubMedGoogle Scholar
  90. Tarkowski AK, Witkowska A, Nowicka J (1970) Experimental partheonogenesis in the mouse. Nature 226:162–165PubMedGoogle Scholar
  91. Tarkowski AK, Wojewodzka M (1982) A method for obtaining chimaeric mouse blastocysts with two separate inner cell masses: a preliminary report. J Embryol Exp Morphol 71:215–221PubMedGoogle Scholar
  92. Tarkowski AK, Wroblewska J (1967) Development of blastomeres of mouse eggs isolated at the 4- and 8-cell stage. J Embryol Exp Morphol 18:155–180PubMedGoogle Scholar
  93. Tarkowski AK, Witkowska A, Opas J (1977) Development of cytochalasin B-induced tetraploid and diploid/tetraploid mosaic mouse embryos. J Embryol Exp Morphol 41:47–64PubMedGoogle Scholar
  94. Tarkowski AK, Ozdzenski W, Czołowska R (2001a) Mouse singletons and twins developed from isolated diploid blastomeres supported with tetraploid blastomeres. Int J Dev Biol 45:591–596PubMedGoogle Scholar
  95. Tarkowski AK, Ozdzenski W, Czołowska R (2001b) How many blastomeres of the 4-cell embryo contribute cells to the mouse body? Int J Dev Biol 45:811–816PubMedGoogle Scholar
  96. Tarkowski AK, Ozdzenski W, Czolowska R (2005a) Identical triplets and twins developed from isolated blastomeres of 8- and 16-cell mouse embryos supported with tetraploid blastomeres. Int J Dev Biol 49:825–832PubMedGoogle Scholar
  97. Tarkowski AK, Jagiello K, Czolowska R, Ozdzenski W (2005b) Mouse chimaeras developed from electrofused blastocysts: new evidence for developmental plasticity of the inner cell mass. Int J Dev Biol 49:909–914PubMedGoogle Scholar
  98. Tarkowski AK, Suwinska A, Czolowska R, Ozdzeński W (2010) Individual blastomeres of 16- and 32-cell mouse embryos are able to develop into foetuses and mice. Dev Biol 348:190–198PubMedGoogle Scholar
  99. Torres-Padilla ME, Parfitt DE, Kouzarides T, Zernicka-Goetz M (2007) Histone arginine methylation regulates pluripotency in the early mouse embryo. Nature 445:214–218PubMedGoogle Scholar
  100. Tsunoda Y, McLaren A (1983) Effect of various procedures on the viability of mouse embryos containing half the normal number of blastomeres. J Reprod Fertil 69:315–322PubMedGoogle Scholar
  101. Tsunoda Y, Yasui T, Okubo Y, Nakamura K, Sugie T (1987) Development of one or two blastomeres from eight-cell mouse embryos to term in the presence of parthenogenetic eggs. Theriogenology 28:615–623PubMedGoogle Scholar
  102. Wakayama S, Hikichi T, Suetsugu R, Sakaide Y, Bui H, Mizutani E, Wakayama T (2007) Efficient establishment of mouse embryonic stem cell lines from single blastomeres and polar bodies. Stem Cells 25:986–993PubMedGoogle Scholar
  103. Willadsen SM (1981) The developmental capacity of blastomeres from 4- and 8- cell sheep embryos. J Embryol Exp Morphol 65:165–172PubMedGoogle Scholar
  104. Waksmundzka M, Wisniewska A, Maleszewski M (2006) Allocation of cells in mouse blastocyst is not determined by the order of cleavage of the first two blastomeres. Biol Reprod 75:582–587PubMedGoogle Scholar
  105. Van de Velde H, Cauffman G, Tournaye H, Devroey P, Liebaers I (2008) The four blastomeres of a 4-cell stage human embryo are able to develop individually into blastocysts with inner cell mass and trophectoderm. Hum Reprod 23:1742–1747PubMedGoogle Scholar
  106. Ziomek CA, Johnson MH (1982) The roles of phenotype and position in guiding the fate of 16-cell mouse blastomeres. Dev Biol 91:440–447PubMedGoogle Scholar
  107. Ziomek CA, Johnson MH, Handyside AH (1982) The developmental potential of mouse 16-cell blastomeres. J Exp Zool 221:345–355PubMedGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Department of Embryology, Faculty of BiologyUniversity of WarsawWarsawPoland

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