Preimplantation Mouse Embryo: Developmental Fate and Potency of Blastomeres
During the past decade we have witnessed great progress in the understanding of cellular, molecular, and epigenetic aspects of preimplantation mouse development. However, some of the issues, especially those regarding the nature and regulation of mouse development, are still unresolved and controversial and raise heated discussion among mammalian embryologists. This chapter presents different standpoints and various research approaches aimed at examining the fate and potency of cells (blastomeres) of mouse preimplantation embryo. In dealing with this subject, it is important to recognize the difference between the fate of blastomere and the prospective potency of blastomere, with the first being its contribution to distinct tissues during normal development, and the second being a full range of its developmental capabilities, which can be unveiled only by experimental perturbation of the embryo. Studies of the developmental potential and the fate of blastomeres are of the utmost importance as they may lead to future clinical application in reproductive and regenerative medicine.
KeywordsZona Pellucida Preimplantation Genetic Diagnosis Blastocyst Stage Inner Cell Mass Cdx2 Expression
I would like to dedicate this work to Professor A.K. Tarkowski, my mentor, as an expression of respect for his work and scientific achievements. I am grateful to Professor A.K. Tarkowski, Professor M. Kloc, Dr. J. Kubiak, and Professor M. Maleszewski for critical reading and valuable suggestions. During the preparation of this work Aneta Suwinska was supported by the grant in the framework of PARENT-BRIDGE Programme from the Foundation for Polish Science POMOST/2010-1/9 and a grant from Polish Ministry of Science and Higher Education N N301 311637.
- Guo G, Huss M, Tong GQ, Wang C, Sun LL, Clarke ND, Robson P (2010) Resolution of cell fate decisions revealed by single-cell gene expression analysis from zygote to blastocyst. Dev Cell 18:675–685Google Scholar
- Kelly SJ (1975) Studies of the potency of the early cleavage blastomeres of the mouse. In: Balls M, Wild AE (eds) The early development of mammals. Cambridge University Press, Cambridge, pp 97–105Google Scholar
- McLaren A (1976) Mammalian chimaeras. Cambridge University Press, Cambridge, London, New York, MelbourneGoogle Scholar
- Mintz B (1962) Formation of genotypically mosaic mouse embryos. Am Zool 2:432Google Scholar
- Mullen RJ, Whitten WK, Carter SC (1970) Studies on chimeric mice and half-embryos. In: Annual report of the Jackson Laboratory. Bar harbor, Maine, pp 67–68Google Scholar
- Ozdzeński W, Szczesny E, Tarkowski AK (1997) Postimplantation development of mouse blastocysts with two separate inner cell masses. Anat Embryol (Berl) 195:467–471Google Scholar
- Pedersen RA, Spindle AI, Wiley LM (1977) Regeneration of endoderm by ectoderm isolated from mouse blastocysts. Nature 270:453–457Google Scholar
- Seidel F (1952) Die entwicklugspotenzen einen isolierten blastomere des zweizellen-stadiums im saugetierei. Naturwissenschaften 39:355–356Google Scholar
- Seidel F (1960) Die entwicklungsfahigkeiten isolierter furchungszellen aus dem ei des kaninchens Oryctolagus cuniculus. Roux Arch Entw Mech 152:43–130Google Scholar
- Staessen C, Platteau P, Van Assche E, Michiels A, Tournaye H, Camus M, Devroey P, Liebaers I, Van Steirteghem A (2004) Comparison of blastocyst transfer with or without preimplantation genetic diagnosis for aneuploidy screening in couples with advanced maternal age: a prospective randomized controlled trial. Hum Reprod 19:2849–2858PubMedGoogle Scholar
- Tarkowski AK (1959b) Experimental studies on regulation in the development of isolated blastomeres of mouse eggs. Acta Theriol 3:191–267Google Scholar