Abstract
Lipoproteins were separated from serum of normal healthy volunteers by sequential ultracentrifugation (Aviram, Biochem Med 30:111–118, 1983) and dialyzed against saline with EDTA (1 mM) for removing excess salt. Protein concentration of the separated fraction was determined by the lowry method (Lowry et al J Biol Chem 193:265–275, 1951). Translation to molar concentrations was calculated using a protein weight of 500 kDa for LDL. For HDL an average particle weight of 360 kDa and 45 % protein content was assumed for calculations.
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Haber, A. (2012). Materials and Methods. In: Metallocorroles for Attenuation of Atherosclerosis. Springer Theses. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-30328-9_6
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DOI: https://doi.org/10.1007/978-3-642-30328-9_6
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