Metabolic Engineering of Plant Cellular Metabolism: Methodologies, Advances, and Future Directions

  • Rafael Zárate
  • Nabil el Jaber-Vazdekis
  • Robert Verpoorte


Plant natural products are useful compounds, such as pigments, fragrances, pharmaceuticals used for the treatment of several human diseases. Development of plant manipulation techniques, such as particle bombardment, Agrobacterium-mediated transformation, vacuum infiltration, agrodrench, viral vector, protoplasts fusion and ultrasound, as well as recombinant DNA, and genetic technologies applied toward metabolic engineering of bioactive plant natural products are presented, together with different genetic engineering methods, such as overexpression of transgenes, multiple expression of transgenes, gene silencing, and transcription factors as powerful tools for the engineering of biosynthetic pathways. Future perspectives and the potential of different approaches are presented to highlight how a better understanding of secondary metabolite pathways represents a direct successful highway to genetic manipulation of desired metabolic pathways.


Hairy Root Metabolic Engineering Indole Alkaloid Tobacco Rattle Virus Vacuum Infiltration 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.



The authors would like to thank Dr. Suman Chandra for the invitation to contribute to this book. RZ and NJV acknowledge Instituto Canario de Investigación del Cáncer and Agencia Canaria de Investigación Innovación y Sociedad de la Información, Spain for financial support.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  • Rafael Zárate
    • 1
  • Nabil el Jaber-Vazdekis
    • 1
  • Robert Verpoorte
    • 2
  1. 1.Instituto Canario de Investigación del Cáncer, Hospital Universitario La CandelariaSanta Cruz de TenerifeSpain
  2. 2.Natural Products LaboratoryInstitute of Biology Leiden, University of LeidenLeidenThe Netherlands

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