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Downstream Processes for Plant Cell and Tissue Culture

  • Ozlem Yesil-Celiktas
  • Fazilet Vardar-Sukan
Chapter

Abstract

Biotechnological cultivation of plant cells and tissues is one of the most challenging fields of recent scientific researches. The requirements of pharmaceutical and food industries in terms of value-added phytochemicals have increased rapidly in the last few years. Therefore, cost-effective and technically sound downstream processes have to be developed in order to supply standardized end-products. Depending on the utilization of the biomass or the culture broth, different types of facilities are needed for the recovery and quali-quantitative analysis. The aim of this chapter is to summarize mass transfer considerations and critically outline the developments in downstream processing of plant cell and tissue cultures by presenting fundamental knowledge as well as the advantages and disadvantages of some conventional and novel techniques such as supercritical fluid and membrane processes. Furthermore, state of the art for recovery and purification of secondary metabolites were discussed in the light of patents via Espacenet database covering US, Japan, European, Korean, and Chinese issued and applied patents and WIPO publications. Innovative downstream processes in combination with hyphenated techniques representing a modern approach to perform fast and reproducible analytical methods for quantification and quality assurance of secondary metabolites are major challenges for industrial-scale applications of plant cell and tissue cultures.

Keywords

Suspension Culture Hairy Root Supercritical Fluid Extraction Rosmarinic Acid Plant Cell Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

References

  1. Abdelkar MSA, Lockwood B, Sansongsak P (2010) Uptake of nicotine from suspension culture of Nicotiana tabacum by molecularly imprinted polymers. J Pharm Pharmacol 62:633–637Google Scholar
  2. Alami I, Mari S, Clerivet A (1998) A glycoprotein from Ceratocystis fimbriata f. sp. platani triggers phytoalexin synthesis in Platanus x acerifolia cell-suspension cultures. Phytochemistry 48:771–776CrossRefGoogle Scholar
  3. Alexey E (2004) Charged cyclodextrin derivatives and their use in plant cell and tissue culture growth media. AU 2003298824 A1 20040623Google Scholar
  4. Alpay E, Demircioglu M (2006) Mass transfer and mass transfer operations. Ege University publication, IzmirGoogle Scholar
  5. Castor TP, Chikarmane HM, Hong GT, Shallice C (1998) Methods for fraction of biological derived materials. USP, 5854064Google Scholar
  6. Chan DJC, Ahmad AL, Chan LK (2010) Improvement of biomass and juvenile hormone III (JH III) production from Cyperus aromaticus cell suspension culture via in situ membrane filtration technology. Acta Physiol Plant 32:1153–1159CrossRefGoogle Scholar
  7. Charoenrat T, Ketudat-Cairns M, Jahic M, Enfors SO, Veide A (2006) Recovery of recombinant beta-glucosidase by expanded bed adsorption from Pichia pastoris high-cell-density culture broth. J Biotechnol 122:86–98PubMedCrossRefGoogle Scholar
  8. Chaterjee A, Shukla S, Mishra P, Rastogi A, Singh SP (2010) Prospects of in vitro production of thebaine in opium poppy (Papaver somniferum L.). Ind Crops Prod 32:668–670CrossRefGoogle Scholar
  9. Chen SQ (2005) Plant cell tissue simple-pouring bioreactor culture system CN 1710058 A 20051221Google Scholar
  10. Chiang L, Abdullah MA (2007) Enhanced anthraquinones production from adsorbent-treated Morinda elliptica cell suspension cultures in production medium strategy. Process Biochem 42:757–763CrossRefGoogle Scholar
  11. Choi JW (1992) In situ berberine separation with immobilized adsorbent in cell-suspension cultures of Thalictrum rugosum. Korean J Chem Eng 9:128–134CrossRefGoogle Scholar
  12. Corry JP, Reed WL, Curtis WR (1993) Enhanced recovery of solavetivone from Agrobacterium transformed root cultures of hyoscyamus-muticus using integrated product extraction. Biotechnol Bioeng 42:503–508PubMedCrossRefGoogle Scholar
  13. Curino A, Milanesi L, Benassati S, Skliar M, Boland R (2001) Effect of culture conditions on the synthesis of vitamin D-3 metabolites in Solanum glaucophyllum grown in vitro. Phytochemistry 58:81–89PubMedCrossRefGoogle Scholar
  14. Darani KK, Farahani EV (2005) Application of supercritical fluid extraction in biotechnology. Crit Rev Biotechnol 25:231–242CrossRefGoogle Scholar
  15. Dierkes H, Steinhagen V, Bork M, Lutge C, Knez Z (2011) Cell lysis of plant or animal starting materials by a combination of a spray method and decompression for the selective extraction and seperation of valuable intracellular materials EP 2315825 A1 20110504Google Scholar
  16. Dobbs JM, Wong JM, Lahiere RJ, Johnston KP (1987) Modification of supercritical fluid phase-behavior using polar cosolvents. Ind Eng Chem Res 26:56–65CrossRefGoogle Scholar
  17. Donnez D, Kim KH, Antoine S, Conreux A, De Luca V, Jeandet P (2011) Bioproduction of resveratrol and viniferins by an elicited grapevine cell culture in a 2 L stirred bioreactor. Process Biochem 46:1056–1062CrossRefGoogle Scholar
  18. Gallois R, Prevot JC, Clement A, Jacob JL (1997) Purification and characterization of phosphoribosylpyrophosphate synthetase from rubber tree latex. Plant Physiol 115:847–852PubMedGoogle Scholar
  19. Ghosh A, Ganapathi TR, Nath P, Bapat VA (2009) Establishment of embryogenic cell suspension cultures and Agrobacterium-mediated transformation in an important Cavendish banana cv. Robusta (AAA) Plant Cell Tiss Org 97:131–139Google Scholar
  20. Gichner T, Stavreva DA, Cerovskan N, Wagner ED, Plewa MJ (1995) Metabolic-Activation of m-phenylenediamine to products mutagenic in Salmonella typhimurium by medium isolated fiom tobacco Suspension Cell-Cultures. Mutat Res-Fund Mol M 331:127–132CrossRefGoogle Scholar
  21. Gromova M, Roby C (2010) Toward Arabidopsis thaliana hydrophilic metabolome: assessment of extraction methods and quantitative 1H NMR. Physiol Plantarum 140:111–127CrossRefGoogle Scholar
  22. Gurel A (1989) The Effects of Plant Growth Regulators on The Production of Dihaploids of Tobacco and Datura through Callus Culture, PhD thesis, Ege University Graduate School of Natural and Applied Sciences, p.101, BornovaGoogle Scholar
  23. Harashima H, Kato K, Shinmya A, Sekine M (2007) Auxin is required for the assembly of A-type cyclin-dependent kinase complexes in tobacco cell suspension culture. J Plant Physiol 164:1103–1112PubMedCrossRefGoogle Scholar
  24. Hartonen K, Parshntsev J, Sandberg K, Bergelin E, Nisula L, Riekkola M-L (2007) Isolation of flavonoids from aspen knotwood by pressurized hot water extraction and comparison with other extraction techniques. Talanta 74:32–38PubMedCrossRefGoogle Scholar
  25. Hellwig S, Drossard J, Twyman RM, Fischer R (2004) Plant cell cultures for the production of recombinant proteins. Nat Biotechnol 22:1415–1422PubMedCrossRefGoogle Scholar
  26. Hong HJ, Lee JE, Ahn JE, Kim DI (1998) Enhanced production of digoxin by digitoxin biotransformation using in situ adsorption in Digitalis lanata cell cultures. J Microbiol Biotechnol 8:478–483Google Scholar
  27. Hwang SY, Lee EK, Terashima M (2005) Chromatography based purification of rhGM-CSF from recombinant rice cell culture. Chem Eng Technol 28:1421–1425CrossRefGoogle Scholar
  28. Jo SH, Kwon SY, Park DS, Yang KS, Kim JW, Lee KT, Kwak SS, Lee HS (2006) High-yield production of functional human lactoferrin in transgenic cell cultures of Siberian ginseng (Acanthopanax senticosus) Biotechnol. Bioprocess Eng 11:442–448CrossRefGoogle Scholar
  29. Kaiser CS, Römpp H, Schmidt PC (2001) Pharmaceutical Applications of Supercritical Carbon Dioxide. Pharmazie 56:907–926PubMedGoogle Scholar
  30. Kang SH, Kim JH, Mun S (2010) Optimal design of a tandem simulated moving bed process for separation of paclitaxel, 13-dehydroxybaccatin III, and 10- deacetylpaclitaxel. Process Biochem 45:1468–1476CrossRefGoogle Scholar
  31. Kim TH, Shin JH, Baek HH, Lee HJ (2001) Volatile flavour compounds in suspension culture of Agastache rugosa Kuntze (Korean mint). J Sci Food Agric 81:569–575CrossRefGoogle Scholar
  32. Kurosumi A, Kobayashi F, Mtui G, Nakamura Y (2006) Development of optimal culture method of Sparassis crispa and a new extraction method of antineoplastic constituent. Biochem Eng J 30:109–113CrossRefGoogle Scholar
  33. Kwon TH, Yang MS (2004) Production method of a large amount of biological active interleukin-12 protein using plant cell suspention culture cheaply and safely. KR 20040097575 A 20041118Google Scholar
  34. Machado MW, Neto CS, Salgado J, Zaffari G, Barison A, Campos FR, de Corilo YE, Eberlin MN, Biavatti MW (2010) Search for alkaloids on callus culture of Passiflora alata. Brazillian Arch Biol Technol 53:901–910CrossRefGoogle Scholar
  35. Masse JP (1991) Therapeutic composition containing alpha-linolenic acid and a compound capable of promoting the passage of the acid through the cell membrane, plant extract comprising the acid and the compound, and process for the preparation of the extract, US 5002767 A 19910326Google Scholar
  36. McHugh MA, Krukonis VJ (1994) Supercritical fluid extraction. Principles and practice, 2nd edn. Butterworth-Heinemann, StonehamGoogle Scholar
  37. Nakamura S, Hayashi T (1993) Purification and properties of an extracellular endo-1, 4-beta-glucanase from suspension-cultured poplar cells. Plant Cell Physiol 34:1009–1013Google Scholar
  38. Nikolov ZL, Woodard SL (2004) Downstream processing of recombinant proteins from transgenic feedstock. Curr Opin Biotechnol 15:479–486PubMedCrossRefGoogle Scholar
  39. Ogawa K, Matsushita T, Funatsu K (1991) Establishment of a simple separation process for highly pure beta-carotene from cultured carrot cells. Kagaku Kogaku Ronbunshu 17:437–441CrossRefGoogle Scholar
  40. Prakash G, Srivastava AK (2007) Azadirachtin production in stirred tank reactors by Azadirachta indica suspension culture. Process Biochem 42:93–97CrossRefGoogle Scholar
  41. Pyo SH, Song BK, Han BH, Kim JH (2004) A large-scale purification of paclitaxel from cell cultures of Taxus chinensis Process Biochem 39:1985–1991Google Scholar
  42. Rivzi SSH, Benado AL, Zollweg JA, Daniels JA (1986) Supercritical fluid extraction: Fundamental principles and modelling methods. Food Technol 7:55–65Google Scholar
  43. Salgin U, Ustun AS, Mehmetoglu U, Calimli A (2005) Supercritical CO2 extraction of accumulated capsidiol from biotic elicitor-activated Capsicum annuum L fruit tissues. J Chem Technol Biotechnol 80:124–132CrossRefGoogle Scholar
  44. Sandell J (1996) Balancing market and technology requirements in early stage product development. Dissertation, Department of Industrial Marketing and Economics, Chalmers University of Technology, GoteborgGoogle Scholar
  45. Schmidt SA, Williams SJ, Wang CIA, Sornaraj P, James B, Kobe B, Dodds PN, Ellis JG, Anderson PA (2007) Purification of the M flax-rust resistance protein expressed in Pichia pastoris. Plant J 50:1107–1117PubMedCrossRefGoogle Scholar
  46. Seader JD, Henly EJ (1998) Separation process principles. Wiley, USAGoogle Scholar
  47. Sim SJ, Chang HN, Liu JR, Jung KH (1994) Production and secretion of indole alkaloids in hairy root cultures of Catharanthus roseus—effects of in situ adsorption, fungal elicitation and permeabilization. J Ferment Bioeng 78:229–234CrossRefGoogle Scholar
  48. Soletto D, Binaghi L, Lodi A, Carvalho JCM, Converti A (2005) Batch and fed-batch cultivations of Spirulina platensis using ammonium sulphate and urea as nitrogen sources. Aquaculture 243:217–224CrossRefGoogle Scholar
  49. Son SH, Choi SM, Lee YH, Choi KB, Yun SR, Kim JK, Park HJ, Kwon OW, Noh EW, Seon JH, Park YG (2000) Large-scale growth and taxane production in cell cultures of Taxus cuspidata (Japanese yew) using a novel bioreactor. Plant Cell Rep 19:628–633CrossRefGoogle Scholar
  50. Su WW, Lei F, Kao NP (1995) High density cultivation of Anchusa officinalis in a stirred-tank bioreactor with in situ filtration. Appl Microbiol Biotechnol 44:293–299CrossRefGoogle Scholar
  51. Sutor R, Kreis W (1996) Partial purification and characterization of the cell-wall-associated lanatoside 15′-O-acetylesterase from Digitalis lanata suspension cultures. Plant Physiol Biochem 34:763–770Google Scholar
  52. Takahashi T, Warren GS (1994) Studies on an inhibitor of TMV viral-antigen accumulation released by cultured-cells of Nicotiana-tabacum. Plant Sci 100:83–96CrossRefGoogle Scholar
  53. Tanaka H, Jajima M (1985) Extraction of material produced in plant cell. JP 60180594 A 19850914Google Scholar
  54. Tapia JC, Garcia R, Escamilla EM, Calva G, Rocha JA (1993) Capsaicin recovery from a cell-culture broth. Ind Eng Chem Res 32:2242–2246CrossRefGoogle Scholar
  55. Taylor LT (1996) Supercritical fluid extraction. Wiley, USAGoogle Scholar
  56. Thibault JF, Della VG, Ralet MC (1991) Process for treating products rich in plant cell walls, in particular rich in pectins, said pectins, the extraction residues from the process, their use and compositions containing them. EP 0432210 A1 19910619Google Scholar
  57. Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631PubMedCrossRefGoogle Scholar
  58. Thorpe TA (2007) History of plant tissue culture. Mol Biotechnol 37:69–180CrossRefGoogle Scholar
  59. Tikhomiroff C, Allais S, Klvana M, Hisiger S, Jolicoeur M (2002) Continuous selective extraction of secondary metabolites from Catharanthus roseus hairy roots with silicon oil in a two-liquid-phase bioreactor. Biotechnol Prog 18:1003–1009PubMedCrossRefGoogle Scholar
  60. Venkatramesh M, Wagner D, Lall S, Lejard F (2010) Production and extraction of procyanidins from plant cell cultures. WO 2010114567 A1 20101007Google Scholar
  61. Wang Y, Zhao B, Ouyang F (1999) Airlift cyclic immersion lighting plant cell tissue organ culture method and culture reactor CN 1063224 C 20010314Google Scholar
  62. Wang CG, Wu JY, Mei XG (2001) Enhanced taxol production and release in Taxus chinensis cell suspension cultures with selected organic solvents and sucrose feeding. Biotechnol Prog 17:89–94PubMedCrossRefGoogle Scholar
  63. Warzecha H, Obitz P, Stockigt J (1999) Purification, partial amino acid sequence and structure of the product of raucaffricine-O-beta-D-glucosidase from plant cell cultures of Rauwolfia serpentine. Phytochemistry 50:1099–1109PubMedCrossRefGoogle Scholar
  64. Williams RD, Chauret N, Bedard C, Archambault J (1992) Effect of polymeric adsorbents on the production of sanguinarine by Papaver somniferum cell-cultures. Biotechnol Bioeng 40:971–977PubMedCrossRefGoogle Scholar
  65. Winkelnkemper T, Schuldt S, Schembecker G (2011) Systematic downstream process development for purification of baccatin III with key performance indicators. Sep Purif Technol 77:355–366CrossRefGoogle Scholar
  66. Xu XH, Zhang W, Cao XP, Xue S (2011) Abietane diterpenoids synthesized by suspension-cultured cells of Cephalotaxus fortunei. Phytochem Lett 4:52–55CrossRefGoogle Scholar
  67. Yang MS (2011) Method of producing humanized fragment antibody via transgenic plant cell suspention culture. KR 20100125870 A 20101201Google Scholar
  68. Yatian D (2008) Curtain-shape anchoring culture bioreactor and method for culturing plant cell, tissue or organ using the same CN 101100641 A 20080109Google Scholar
  69. Yesil-Celiktas O, Nartop P, Gurel A, Bedir E, Vardar Sukan F (2007) Determination of phenolic content and antioxidant activity of extracts obtained from Rosmarinus officinalis’ calli. J Plant Physiol 164:1536–1542PubMedCrossRefGoogle Scholar
  70. Yesil-Celiktas O, Gurel A, Vardar-Sukan F (2010) Large scale cultivation of plant cell and tissue culture in bioreactors. Transworld Research Network, KeralaGoogle Scholar
  71. Zhang W, Franco CMM (2005) Production of plant secondary metabolites using adsorption and elicitation in cell suspention culture. WO 2005012507 A1 20050210Google Scholar
  72. Zhang CH, Xu HB (2001) Improved paclitaxel production by in situ extraction and elicitation in cell suspension cultures of Taxus chinensis. Biotecnol Lett 23:189–193CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 2013

Authors and Affiliations

  1. 1.Department of BioengineeringEge UniversityBornovaTurkey

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