Genome Engineering of Corynebacterium glutamicum
As a direct consequence of the recent advances in DNA sequencing technologies, complete genome sequences of more than 1,200 bacterial species have already been deciphered and they form an important resource for understanding the diversity of bacterial metabolic systems. Manipulation of bacterial genome sequences by integration, replacement, and disruption of individual genes has, in parallel, become a powerful strategy to improve bacterial traits. Regarding Corynebacterium glutamicum, whole genome sequences of two strains, namely R (3,314,179 bp) and ATCC 13032 (3,309,401 bp or 3,282,708 bp), have been determined and strain reconstruction studies initiated. Several techniques for genome-wide genetic manipulations using transposons, DNA recombinase, and homologous recombination reactions have been developed. These advances are particularly important because C. glutamicum has a long history of applications for the production for various commodity and fine chemicals. Armed with the microbe’s complete sequence, improvement and tailoring of its properties using genome engineering techniques continue to help facilitate the identification of metabolic bottlenecks and, consequently, their resolution. This in turn enhances the intrinsic characteristics of this bacterium as an industrial workhorse. In this chapter, recently developed techniques that enable to manipulate the C. glutamicum genome are summarized.
KeywordsTransposable Element Corynebacterium Glutamicum loxP Site Restriction Barrier Transposition Efficiency
We wish to thank Dr. C. Omumasaba (internal) for the critical reading of the manuscript.
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