Molecular Biology Methods in the Diagnosis of Multiple Myeloma

  • Anna Dmoszyńska
  • Sylwia Chocholska
Part of the Principles and Practice book series (PRINCIPLES)


The development of multiple myeloma (MM) is a multistep process including sequential series of genetic abnormalities. Failure to obtain informative metaphases from conventional cytogenetic techniques might be overcome by using molecular cytogenetic methods, which utilize DNA from interphase nuclei, thus permitting the evaluation of chromosomal regions in non-dividing cells. The advent of molecular biology methods, such as fluorescence in situ hybridization (FISH), comparative genomic hybridization (CGH) or gene expression profiling (GEP) has provided new insights into the biology of MM. These methods may help to explain clinical heterogeneity of MM and to differentiate between the disease subtypes related to good and bad prognosis.


Multiple Myeloma Bone Marrow Sample Phosphate Buffer Saline Solution Molecular Biology Method Bone Marrow Plasma Cell 
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© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Department of Hematooncology and Bone Marrow TransplantationMedical University of LublinLublinPoland

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