Screen of Streptomyces Hygroscopicus BOS-013 Strain and Identification of the Antifungal Antibiotic from Its Fermentation Broth
BOS-013 actinomycete strain was obtained by separating different kinds of soils from the nature. Moreover, the morphology, cultural characteristics, physiological-biochemical characteristics and 16S rDNA sequences of this strain were studied. A phylogenetic tree was constructed by comparing with the published 16S rDNA sequences of the related species and showed 99% identity of nucleotide sequence of 16S rDNA with Streptomyces hygroscopicus. The antifungal Biotic produced by Streptomyces Hygroscopicus BOS-013 was purified by means of macro-porous adsorbent resin, and the crystal of the antibiotic with high purity was got. In this paper, the methods of purification by adsorbing of microporous adsorbent resin and detection by high performance liquid chromatography with mass spectrum (HPLC-MS) were established. The study would be laid a good foundation for its physical-chemical properties. Meanwhile, we identified its structure by spectral analyses. The result appeared that the optimal eluant was 50% ethanol. The best developing agent in thin-layer chromatography was Petroleum ether: ethyl acetate (5:1, v / v). Quasi-molecular ion peak [M+H]+ was 547 given by positive ion mode-electrospray ionization-mass spectrometry and pushed-out its molecular weight was 546. Meanwhile we exserted its molecular formula was C30H43O9 combined with NMR spectrum. We did not find this compound, so it was infered as a new compound temporarily after Scifinder search.
KeywordsStreptomyces Screen Antibiotic dentification Fermentation Broth
Unable to display preview. Download preview PDF.
- 2.Institute of Microbiology, Chinese Academy of taxonomic groups of actinomycetes. Streptomyces identification manual, pp. 13–15. Science Press, Beijing (1975) Google Scholar
- 3.XuPing, Li, W.-J., Xu, L.-H., et al.: MICROWAVE method for rapid extraction of actinomycetes genome DNA. Microbiology 30(4), 82–84 (2003)Google Scholar
- 5.Ogawara, H., Kawamura, N., Kudo, T., et al.: Distribution of β-lactamases in actinomycetes. Antimicrob. Agents Chemother. 43, 3014–3017 (1999)Google Scholar