Diagnostic Magnetic Resonance Technology
For the sensitive and quantitative measurement of protein biomarkers, pathogens, and cells in clinical samples, magnetic nanoparticles (MNPs) offer unique advantages over traditional detection methods. Specifically, due to the inherently negligible magnetic background of biological material, MNPs can be used to obtain highly sensitive measurements in minimally processed samples. Our detection platform, termed diagnostic magnetic resonance (DMR), exploits MNPs to modulate the nuclear magnetic spin-spin relaxation time of water. Here, we review work done by our group to develop more effective MNP biosensors, advanced conjugational strategies to target the MNPs to molecular targets, and highly sensitive miniaturized NMR systems. We demonstrate this platform as a robust and easy-to-use system for the detection of a wide range of targets in clinical settings including whole cells, proteins, DNA/mRNA, metabolites, drugs, viruses, and bacteria.
KeywordsNuclear Magnetic Resonance Nuclear Magnetic Resonance Data Nuclear Magnetic Resonance Signal Solenoidal Coil Nuclear Magnetic Resonance Probe
We thank T. J. Yoon, J. H. Chung, J. B. Haun, and N. Sergeyev for assistance with experiments and R. M. Westervelt (Harvard) and C. Castro (MGH) for many helpful discussions. This work was supported in part by the following NIH grants: U54CA151884, R01EB010011, R01EB004626, P01CA069246, P50CA86355, U01CA141556, U24CA092782, and R21CA14122. H. Shao acknowledges financial support from the B.S.-Ph.D. National Science Scholarship awarded by the Agency for Science, Technology and Research, Singapore.
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