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Studying Membrane Transport Processes by Non-invasive Microelectrodes: Basic Principles and Methods

  • Sergey Shabala
  • Lana Shabala
  • Ian Newman
Chapter

Abstract

Non-invasive microelectrode flux measurement is a convenient tool to study membrane transport processes in plants in situ. Its high resolution (a few microns in space and several seconds in time) enables real-time studies of membrane transport processes at various levels of plant structural organization, from intact organs to various tissues, single cells or protoplasts derived from these cells. Being noninvasive, the technique allows flux measurements over several hours or days, even from rapidly moving tissues such as growing or nutating plant axial organs. Importantly, fluxes of several ions and neutral molecules can be measured concurrently, enabling not only quantitative estimates of rates of measured processes, but also providing some valuable information about the stoichiometry between activities of various membrane transporters and their time dependence. In this review, we revise the principles of non-invasive ion and neutral molecule flux measurements using the MIFE technique and discuss some methodological aspects of manufacturing, calibrating, and using flux measuring microelectrodes.

Keywords

Flux Measurement Flux Equation Nernst Slope Electrode Holder Membrane Transport Process 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Abbreviations

LIX

Liquid ion exchanger

NMR

Nuclear magnetic resonance

Notes

Acknowledgments

This work was supported by an Australian Research Council grant to Prof Sergey Shabala.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.School of Agricultural ScienceUniversity of TasmaniaHobartAustralia
  2. 2.School of Mathematics and PhysicsUniversity of TasmaniaHobartAustralia

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