Abstract
CRISPR is a recently discovered adaptive prokaryotic immune system. A crucial step in CRISPR defense mechanism is transcription of CRISPR cassette, which is followed by processing of the resulting long transcript (pre-crRNA) into small RNA molecules (crRNA) that recognize invading viruses. We model CRISPR transcript processing, and show that the system functions as a strong amplifier, which can rapidly generate a large number of crRNAs from only few pre-crRNA molecules. Based on this analysis, we propose a synthetic gene circuit that can produce a large number of desired molecules from a potentially toxic substrate.
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References
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© 2012 Springer-Verlag Berlin Heidelberg
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Djordjevic, M., Severinov, K., Djordjevic, M. (2012). CRISPR Transcript Processing: An Unusual Mechanism for Rapid Production of Desired Molecules. In: Lones, M.A., Smith, S.L., Teichmann, S., Naef, F., Walker, J.A., Trefzer, M.A. (eds) Information Processign in Cells and Tissues. IPCAT 2012. Lecture Notes in Computer Science, vol 7223. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28792-3_5
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DOI: https://doi.org/10.1007/978-3-642-28792-3_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-28791-6
Online ISBN: 978-3-642-28792-3
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