Abstract
Emerging interest of economic biobutanol production at industrial level is being stimulated owing to flourishing environmental issues and hiking of price for petroleum-based liquid fuels due to continuous depletion of oil reserves. Moreover, biobutanol also demonstrated various significant properties over bioethanol (commercialized biofuel) such as high calorific value, low freezing point, high hydrophobicity, low heat of vaporization, no need of modification in exiting car engines, less corrosive, no blending limit (can be used up to 100%), its dibutyl ether derivative has potential for diesel fuel, etc. Unfortunately, economic feasibility of biobutanol fermentation is suffering due to low butanol titer as butanol itself acts as inhibitor during fermentation. To overcome this problem several genetic and metabolic engineering strategies are being tried. Still, none of the attempts are successful efficiently as butanol disrupts the cytoplasmic membrane and its functions, which are essential for survival of organism. Therefore, online product recovery technologies with continuous fermentation are being optimized to enhance the butanol productivity. However, studies based on economic evaluation of biobutanol production illustrated that production cost of biobutanol primarily depends on cost of raw material. In this direction, conversion of cheaper lignocellulosic biomass (agriculture waste and wood residue) to biobutanol is promising the great potential towards the economic feasibility of this liquid fuel.
Keywords
- Wheat Straw
- Switch Grass
- Corn Stover
- Lignocellulosic Biomass
- Lignocellulosic Material
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, access via your institution.
Buying options



References
Hoekman SK (2009) Biofuels in the U.S.—challenges and opportunities. Renew Energy 34:14–22
Antoni D, Zverlov VV, Schwarz WH (2007) Biofuels from microbes. Appl Microb Biotechnol 77:23–35
Yang QZ, Song B (2008) Sustainability assessment of biofuels as alternative energy resources. ICSET
Huang H, Liu H, Gan YR (2010) Genetic modification of critical enzymes and involved genes in butanol biosynthesis from biomass. Biotechnol Adv. doi:10.1016/j.biotechadv.2010.05.015
Ma H, Oxley L, Gibson J, Li W (2010) A survey of China’s renewable energy economy. Renew Sustain Energy Rev 14:438–445
Demirdas A (2009) Biofuels securing the planet’s future energy needs. Energy Convers Manag 50:2239–2249
Demirdas A (2009) Political, economic and environmental impacts of biofuels: a review. Appl Energy 86:108–117
Kent SK (2009) Biofuels in the U.S.—challenges and opportunities. Renew Energy 34:14–22
Naik SN, Goud VV, Rout PK, Dalai AK (2010) Production of first and second generation biofuels: a comprehensive review. Renew Sustain Energy Rev 14:578–597
Gressel J (2008) Transgenics are imperative for biofuel crops. Plant Sci 174:246–263
Zhou A, Thomson E (2009) The development of biofuels in Asia. Appl Energy 86:11–20
Yan J, Lin T (2009) Biofuels in Asia. Appl Energy 86:1–10
Durre P (2007) Biobutanol: an attractive biofuel. Biotechnol J 2:1525–1534
Pfromm PH, Boadu VA, Nelson R, Vadlani P, Madl R (2010) Bio-butanol vs. bio-ethanol: a technical and economic assessment for corn and switchgrass fermented by yeast or Clostridium acetobutylicum. Biomass Bioenergy. doi:10.1016/j.biombioe.2009.12.017
Jones DT, Woods DR (1986) Acetone-Butanol fermentation revisited. Microbiol Rev 50(4):484–524
Durre P (1998) New insights an novel developments in clostridial acetone/butanol/isopropane fermentation. Appl Microb Biotechnol 49:639–648
Keis S, Shaheen R, Jones TD (2001) Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov. Int J Syst Evol Microbiol 51:2095–2103
Comyns AE (2006) BP and Du plan ‘biobutanol’. Focus on Catalysts 2006(8):7
Comyns AE (2007) Bio-futures. Focus Catalysts 2007(8):1
Comyns AE (2009) Biobutanol: a European jv for BP and DuPont. Focus Catalysts 2007(8):3
Zhang Z, Lohr L, Escalante C, Wetzstein M (2010) Food versus fuel: what do prices tell us? Energy Policy 38:445–451
Qureshi N, Saha BC, Hector RE, Dien B, Hughes S, Liu S, Iten L, Bowman MJ, Sarath G, Cotta MA (2010) Production of butanol (a biofuel) from agricultural residues: part II—use of corn stover and switchgrass hydrolysates. Biomass Bioenergy 35:559–669
Qureshi N, Saha BC, Hector RE, Hughes SR, Cotta MA (2008) Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii: part I—batch fermentation. Biomass Bioenergy 32:168–175
Qureshi N, Saha BC, Cotta MA (2008) Butanol production from wheat straw by simultaneous saccharification and fermentation using Clostridium beijerinckii: part II—fed-batch fermentation. Biomass Bioenergy 32:176–183
Qureshi N, Ezeji TC, Ebener J, Dien BS, Cotta MA, Blaschek HP (2008) Butanol production by Clostridium beijerinckii. Part I: use of acid and enzyme hydrolyzed corn fiber. Bioresourse Technol 99:5915–5922
Qureshi N, Saha BC, Dien B, Hector RE, Cotta MA (2010) Production of butanol (a biofuel) from agricultural residues: part I—use of barley straw hydrolysate. Biomass Bioenergy 34:559–565
Larson DE (2008) Biofuel production technologies: status, prospects and implications for trade and development. In: United Nations conference on trade and development, 2008, New York and Geneva
Chauhan S (2010) Biomass resources assessment for power generation: a case study from Haryana state, India. Biomass Bioenergy 34:1300–1308
Badr HR, Toledo R, Hamdy MK (2001) Continuous acetone ethanol butanol fermentation by immobilized cells of Clostridium acetobutylicum. Biomass Bioenergy 20:119–132
Huang WC, Ramey DE, Yang ST (2004) Continuous production of butanol by Clostridium acetobutylicum immobilized in a fibrous bed bioreactor. Appl Biochem Biotechnol 115:887–898
Nimcevic D, Gapes JR (2000) The acetone-butanol fermentation in pilot plant and pre-industrial scale. J Mol Microbiol Biotechnol 2(1):15–20
Tashiro Y, Takeda K, Kobayashi G, Sonomoto K, Ishizaki A, Yoshino S (2004) High butanol production by Clostridium saccharoperbutylacetonicum N1-4 in fed-batch culture with pH-stat continuous butyric acid and glucose feeding method. J Biosci Bioeng 98(4):263–268
Qureshi N, Lai LL, Blaschek HP (2004) Scale-up of a high productivity continuous biofilm reactor to produce butanol by adsorbed cells of Clostridium beijerinckii. Food Bioproducts Process 82:164–173
Liew ST, Arbakariya A, Rosfarizan M, Raha AR (2005) Production of solvent (acetone-butanol-ethanol) in continuous fermentation by Clostridium saccharobutylicum DSM 13864 using gelatinised sago starch as a carbon source. Malays J Microbiol 2(2):42–45
Ezeji TC, Qureshi N, Blaschek HP (2005) Continuous butanol fermentation and feed starch retrogradation: butanol fermentation sustainability using Clostridium beijerinckii BA101. J Biotechnol 115:179–187
Ezeji T, Qureshi N, Blaschek HP (2007) Production of acetone–butanol–ethanol (ABE) in a continuous flow bioreactor using degermed corn and Clostridium beijerinckii. Process Biochem 42:34–39
Worden RM, Grethlein AJ, Jain MK, Datta R (1991) Production of butanol and ethanol from synthesis gas via fermentation. Fuel 70(5):615–619
Qureshi N, Maddox IS (1995) Continuous production of acetone-butanol-ethanol using immobilised cells of Clostridium acetobutylicum and intetration with product removal by liquid–liquid extraction. J Ferment Bioengineering 80(2):185–189
Maddox IS, Qureshi N, Thomson KR (1995) Production of Acetone-Butanol-Ethanol from concentrated substrates using Clostridium acetobutylicum in an integrated fermentation-product removal process. Process Biochem 30(3):209–215
Qureshi N, Blaschek HP (2001) Recovery of butanol from fermentation broth by gas stripping. Renew Energy 22:557–564
Qureshi N, Maddox IS (2005) Reduction of butanol inhibition by perstraction: utilization of concentrated lactose/whey permeate by Clostridium acetobutylicum to enhance butanol fermetation economics. Food Bioproducts Process 83:43–52
Assobhei O, Kanouni AE, Ismaili M, Loutfi M, Petitdemange H (1998) Effect of acetic and butyric acids on the stability of solvent and spore formation by Clostridium acetobutylicum ATCC 824 during repeated subculturing. J Ferment Bioeng 85(2):209–212
Evans PJ, Wang HY (1988) Enhancement of butanol formation by Clostridium acetobutylicum in the presence of decanol-oleyl alcohol mixed extractants. Appl Environ Microbiol 54(7):1662–1667
Lin YL, Blaschek HP (1983) Butanol production by a butanol-tolerant strain of Clostridium acetobutyricum in extruded corn broth. Appl Environ Microbiol 45(3):966–973
Johnson JL, Toth J, Santiwatanakul S, Chen JS (1997) Cultures of Clostridium acetobutylicum from various collections comprise Clostridium acetobutylicum, Clostridium beijerinckii, and two other distinct types based on DNA–DNA reassociation. Int J Syst Bacteriol 47(2):420–424
Stefanie K, Shaheen R, Jones TD (2001) Emended descriptions of Clostridium acetobutylicum and Clostridium beijerinckii, and descriptions of Clostridium saccharoperbutylacetonicum sp. nov. and Clostridium saccharobutylicum sp. nov. Int J Syst Evol Microbiol 51:2095–2103
Winzer K, Lorenz K, Zickner B, Durre P (2000) Differential regulation of two thiolase genes from Clostridium acetobutylicum DSM 792. J Mol Microbiol Biotechnol 2(4):531–541
Lenz TG, Morelra AR (1980) Economic evaluation of the acetone-butanol fermentation. Ind Eng Chem Prod Res Dev 19:478–483
Qureshi N, Blaschek HP (2000) Economics of butanol fermentation using hyper-butanol producing Clostridium beijerinckii BA101. Trans I Chem E 78:139–144
Qureshi N, Blaschek HP (2001) ABE production from corn: a recent economic evaluation. J Ind Microbiol Biotechnol 27:292–297
Gapes JR (2000) The economics of acetone-butanol fermentation: theoritical and market considerations. J Microbiol Biotechnol 2(1):27–32
Yan Q, Wang A, Yu W, Wang L (2009) Development strategies of biofuel in China. In: International conference on energy and envioronment technology, 2009. IEEE Computer Society, p 588–593
Ezeji TC, Qureshi N, Blaschek HP (2007) Production of acetone butanol (AB) from liquified corn starch, a commercial substrate, using Clostridium beijerickii coupled with product recovery by gas stripping. J Ind Microbiol Biotechnol 34:771–777
Foda MI, Dong H, Li Y (2010) Study the suitability of cheese whey for bio-butanol production by Clostridia. J Am Sci 6(8):39–46
Ezeji TC, Qureshi N, Blaschek HP (2007) Bioproduction of butanol from biomass: from genes to bioreactors. Curr Opin Biotechnol 18:220–227
Koukiekolo R, Cho HY, Kosugi A, Inui M, Yukawa H, Doi RH (2005) Degradation of corn fiber by Clostridium cellulovorans cellulases and hemicellulases and contribution of scaffolding protein CbpA. Appl Environ Microbiol 71(7):3504–3511
Tao L, Aden A (2009) The economics of current and future biofuels. In Vitro Cell Dev Biology Plant 45:199–217
Qureshi N, Saha BC, Hector RE, Cotta MA (2008) Removal of fermentation inhibitors from alkaline peroxide pretreated and enzymatically hydrolyzed wheat straw: production of butanol from hydrolysate using Clostridiun beijierinckii in batch reactors. Biomass Bioenergy 32:1353–1358
Ezeji TC, Qureshi N, Blaschek HP (2007) Butanol production from agricultural residues: impact of degradation products on Clostridium beijerinkii growth and butanol fermentation. Biotechnol Bioeng 97(6):1460–1469
Qureshi N, Saha BC, Cotta MA (2007) Butanol production from wheat straw hydrolysate using Clostridium beijerinckii. Bioprocess Biosyst Eng 30:419–427
Claassen PAM, Budde MAW, Contreras AML (2000) Acetone, butanol and ethonol production from domestic organic waste by solventogenic Clostridia. J Microbiol Biotechnol 2(1):39–44
Tran HTM, Cheirsilp B, Hodgson B, Umsakul K (2010) Potectial use of Bacillus subtilis in a co-culture with Clostridium butylicum for acetone-butanol-ethanol production from cassava starch. Biochem Eng J 48:260–267
Qureshi N, Blaschek HP (2000) Butanol production using Clostridium beijerinckii BA101 hyper-butanol producing mutant strain and recovery by pervaporation. Appl Biochem Biotechnol 84–86:225–235
Qureshi N, Blaschek HP (2001) Recent advances in ABE fermentation: hyper-butanol producing Clostridium beijerinckii BA101. J Ind Microbiol Biotechnol 27:287–291
Napoli F, Olivieri G, Russo ME, Marzocchella A, Salatino P (2010) Production of butanol in a continuous packed bed reactor of Clostridium acetobutylicum. Chem Eng Trans 20:193–198
Tashiro Y, Takeda K, Kobaayashi G, Sonomoto K (2005) High production of acetone-butanol-ethanol with high cell density culture by cell-recycling and bleeding. J Biotechnol 120:197–206
Qureshi N, Blaschek HP (1999) Production of acetone-butanol-ethanol (ABE) by a hyper-producing mutant strain of Clostridium beijerinckii BA101 and recovery by pervaporation. Biotechnol Prog 15:594–602
Qureshi N, Blaschek HP (2000) Butanol production using Clostridium beijerinckii BA101 hyperbutanol—producing mutant strain and recovery by pervaporation. Appl Biochem Biotechnol 84:5–15
Qureshi N, Schripsema J, Lienhardt J, Blaschek HP (2000) Continuous solvent production by clostridium beijerinckii BA101 immobilized by adsorption on to brck. World J Microbiol Biotechnol 16:377–382
Liu S, Qureshi N (2009) How microbes tolerate ethanol and butanol. New Biotechnol 26:117–121
Schneck KV, Sands JA, Montenecourt BS (1984) Effect of butanol on lipid composition and fluidity of Clostridium acetobutylicum ATCC 824. Appl Environ Microbiol 47(1):193–194
Bowles LK, Ellefson W (1985) Effects of butanol on Clostridium acetobutylicum Appl Environ Microbiol 50(5):1165–1170
Westhuizen AVD, Jones DT, Woods DR (1982) Autolytic activity and butanol tolerance of Clostridium acetobutylicum. Appl Environ Microbiol 44(6):1277–1281
Zheng YN, Li LZ, Xian M, Ma YJ, Yang JM, Xu X, He DZ (2009) Problems with the microbial production of butanol. J Ind Microbiol Biotechnol 36:1127–1138
Ezeji TC, Qureshi N, Blaschek HP (2004) Acetone butanol ethanol (ABE) production from concentrated substrate: reduction in substrate inhibition by fed-batch technique and product inhibition by gas stripping. Appl Microb Biotechnol 63:653–658
Ezeji TC, Qureshi N, Blaschek HP (2003) Production of acetone, butanol and ethanol by Clostridium beijerinckii BA 101 and in situ recovery by gas stripping. World J Microbiol Biotechnol 19:595–603
Qureshi N, Meagher MM, Huang J, Hutkins RW (2001) Acetone butanol ethanol (ABE) recovery by pervaporation using silicalite–silicone composite membrane from fed-batch reactor of Clostridium acetobutylicum. J Membr Sci 187:93–102
Grobben NG, Eggink G, Cuperus FP, Huizing HJ (1993) Production of acetone, butanol and ethanol (ABE) from potato wastes: fermentation with integrated membrane extraction. Appl Microbiol Biotechnol 39:494–498
Qureshi N, Hughes S, Maddox IS, Cotta MA (2005) Energy-efficient recovery of butanol from model solutions and fermentation broth by adsorption. Bioprocess Biosyst Eng 27:215–222
Annous BA, Blaschek HP (1991) Isolation and characterization of Clostridium acetobutylicum mutants with enhanced amylolytic activity. Appl Environ Microbiol 57(9):2544–2548
Formanek J, Mackie R, Blaschek HP (1997) Enhanced butanol production by Clostridium beijerinkii BA101 grown in semi defined P2 medium containing 6 percent maltodextrin or glucose. Appl Environ Microbiol 63(6):2306–2310
Qureshi N, Maddox IS (1992) Application of novel technology to the ABE Fermentation Process. Appl Biochem Biotechnol 34(35):441–448
Demirbas A (2009) Political, Economic and environmental impacts of biofuels: a review. Appl Energy 86:108–117
Author information
Authors and Affiliations
Corresponding author
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 2012 Springer-Verlag Berlin Heidelberg
About this chapter
Cite this chapter
Kumar, M., Gayen, K. (2012). Biobutanol: The Future Biofuel. In: Baskar, C., Baskar, S., Dhillon, R. (eds) Biomass Conversion. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28418-2_7
Download citation
DOI: https://doi.org/10.1007/978-3-642-28418-2_7
Published:
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-28417-5
Online ISBN: 978-3-642-28418-2
eBook Packages: Chemistry and Materials ScienceChemistry and Material Science (R0)