In this chapter, we cover fundamentals of Raman imaging. The different imaging methods, such as point mapping, line scanning and global imaging are described in terms of their advantages and limitations, as well as new imaging modes allowing faster image acquisition. Important definitions of the experiment design, such as spot size, spatial and axial resolution are reviewed. Important concepts enabling to push the limits in terms of spatial resolution are addressed. Finally, basic and more advanced data-processing methods are discussed, as means to separate and eliminate undesirable spectral features. Analysis methods and chemometric tools are introduced, including univariate and multivariate analysis, image and morphological analysis, as well as statistical representation methods. Illustrations are shown with “real-life” samples like pharmaceutical tablets or carbon nanotubes. It concentrates on the empirical ‘good’ practices that can be used in experiment designs.
KeywordsRaman Spectrum Laser Spot Raman Band Active Pharmaceutical Ingredient Hyperspectral Imaging
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