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Advances in Optical Technologies for Rare Cell Detection and Characterization

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Book cover Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer

Part of the book series: Recent Results in Cancer Research ((RECENTCANCER,volume 195))

Abstract

Scanning cytometry enables detection of circulating tumor cells without enrichment, minimizing potential loss of sensitivity due to variable expression of enrichment targets; however, some approaches lack specificity without imaging to identify false positives. High fidelity imaging enables identification of CTCs using morphological considerations and semi-quantitative measurement of biomarker expression for predicting targeted therapy but often lacks speed needed for the large number of mononuclear blood cells. A hybrid approach of first scanning a sample at high speed and high numerical aperture to locate CTCs followed by high resolution imaging of a small number of objects reduces the time needed for high resolution imaging without loss of detection sensitivity.

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Author information

Authors and Affiliations

  1. Kryptos Medical, Los Altos Hills, CA, USA

    Lou Dietz

  2. Palo Alto Research Center, Palo Alto, CA, USA

    Richard Bruce

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  1. Lou Dietz
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Corresponding author

Correspondence to Richard Bruce .

Editor information

Editors and Affiliations

  1. , Breast Cancer Translational Res. Lab., Institut Jules Bordet, Boulevard de Waterloo 125, Brussels, 1000, Belgium

    Michail Ignatiadis

  2. , Breast Cancer Translational Res. Lab., Institut Jules Bordet, Boulevard de Waterloo 125, Brussels, 1000, Belgium

    Christos Sotiriou

  3. Zentrum für Experimentelle Medizin, Inst. Tumorbiologie, Universitätsklinikum Hamburg-Eppendorf, Martinistr. 52, Hamburg, 20246, Germany

    Klaus Pantel

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© 2012 Springer-Verlag Berlin Heidelberg

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Dietz, L., Bruce, R. (2012). Advances in Optical Technologies for Rare Cell Detection and Characterization. In: Ignatiadis, M., Sotiriou, C., Pantel, K. (eds) Minimal Residual Disease and Circulating Tumor Cells in Breast Cancer. Recent Results in Cancer Research, vol 195. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-28160-0_7

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  • DOI: https://doi.org/10.1007/978-3-642-28160-0_7

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  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-28159-4

  • Online ISBN: 978-3-642-28160-0

  • eBook Packages: MedicineMedicine (R0)

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