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Microfluidic Technologies

  • Ali Asgar. S. Bhagat
  • Chwee Teck Lim
Chapter
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 195)

Abstract

Presence of circulating tumor cells (CTCs) in blood is an important intermediate step in cancer metastasis, a mortal consequence of cancer. However, CTCs are extremely rare in blood with highly heterogeneous morphologies and molecular signatures, thus making their isolation technically very challenging. In the past decade, a flurry of new microfluidic-based technologies has emerged to address this compelling problem. This chapter highlights the current state of the art in microfluidic systems developed for CTCs separation and isolation. The techniques presented are broadly classified as physical- or affinity-based isolation depending on the separation principle. The performance of these techniques is evaluated based on accepted separation metrics including sensitivity, purity and processing/analysis time. Finally, further insights associated with realizing an integrated microfluidic CTC lab-on-chip system as an onco-diagnostic tool will be discussed.

Keywords

Circulate Tumor Cell Microfluidic System Hematologic Cell Microfluidic Approach Inertial Lift Force 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  1. 1.Clearbridge BioMedics Pte LtdSingaporeSingapore
  2. 2.Department of BioengineeringNational University of SingaporeSingaporeSingapore
  3. 3.Department of Mechanical EngineeringNational University of SingaporeSingaporeSingapore
  4. 4.Mechanobiology InstituteSingaporeSingapore

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