67Ga and 68Ga Purification Studies: Preliminary Results
The positron emission tomography technique is very useful for diagnosis of several diseases. 68Ga is a positron emitter with half-life of 67.7 min. As it is available from 68Ge/68Ga generator systems, it is not necessary to have a nearby cyclotron. However, the eluate from commercial generators contains high levels of metallic impurities, which compete with 68Ga in biomolecular labeling. Thus, a subsequent purification step is needed after generator elution. Here we present the results of two different methods developed for handmade purification of 68Ga and 67Ga for subsequent radiolabeling of biomolecules. Two purification methods were employed. The first one uses a cation exchange resin, and 68Ga is eluted with a solution of acetone/acid. The second method of purification is performed by column chromatography solvent extraction, with 68Ga recovery in deionized water. The best result was achieved with cationic resin AG50W-X8 (>400 mesh). However, the resin is not commercially available. The extraction chromatography column based on absorption of diisopropyl ether in XAD-16 is the most promising purification method. Although the levels of 68Ga recovery and purification were smaller with the cationic resin method, its advantage is the 68Ga recovery in deionized water.
KeywordsCation Exchange Resin Metallic Impurity Sodium Iodide Diisopropyl Ether Extraction Chromatography Column
- Velikyan I (2005) Synthesis, characterization and application of 68Ga-labelled macromolecules. Dissertation-Uppsala University, SwedenGoogle Scholar