Methods to Isolate and Identify New Plant Signaling Peptides

  • Sunil SagarEmail author
  • Chris Gehring
  • Kenneth P. Minneman
Part of the Signaling and Communication in Plants book series (SIGCOMM, volume 16)


Peptides play a key role in plant development and intercellular communication. A variety of novel peptides and peptidic compounds have been isolated over the last few decades. This chapter examines the various methods used to isolate and identify new peptides and peptidic compounds from plants, and provides an overview of these methods. The principles involved in extraction and methods commonly used for separation, isolation, and structural elucidation are summarized. Methods discussed include high-performance liquid chromatography in both normal and reversed phases, ion-exchange chromatography, size-exclusion chromatography, affinity chromatography, hydrophilic interaction liquid chromatography, hydrophobic interaction chromatography, multidimensional liquid chromatography, and various physical identification techniques such as mass spectrometry for determining molecular structures. The application of such techniques to identification of novel plant peptides is stressed.


Peptide Mixture Hydrophobic Interaction Chromatography Peptide Molecule Peptide Separation Hydrophilic Interaction Liquid Chromatography 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag Berlin Heidelberg 2012

Authors and Affiliations

  • Sunil Sagar
    • 1
    Email author
  • Chris Gehring
    • 2
  • Kenneth P. Minneman
    • 1
  1. 1.Computational Biosciences Research CenterKing Abdullah University of Science and TechnologyThuwalSaudi Arabia
  2. 2.Division of Chemical and Life Sciences and EngineeringKing Abdullah University of Science and TechnologyThuwalSaudi Arabia

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