Advertisement

Insecticidal Activity of Juglans regia Extracts against Tetranychus cinnabarinus and Their Effects on Relative Enzymes Activity in Tetranychus cinnabarinus

  • You-nian Wang
  • Hai-xiang Wang
  • Yu-bo Liu
  • Guang-lu Shi
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 134)

Abstract

The experiment was conducted to determine the insecticidal activity of three different extracts from walnut green husk against Tetranychus cinnabarinus. The results showed that the female insect mortality was 83.44 % at 24 h after exposed in the petroleum ether extract(1 mg·mL-1) from walnut green husk, which was significantly higher than that in the chloroform and methanol extracts (P < 0.05) . After parted and purified by extracting and chromatography, it was found that the acaricidal activity of petroleum ether part from crude extracts was significantly more than the methanol part in the concentration of 1 mg·mL-1, and the female insect mortality reached 84.39 % at 24 h after exposed in the petroleum ether part. There were 19 parts gained from petroleum ether part by chromatography, and the insecticidal activity of 4 parts (6th, 7th, 13th, 17th parts) was higher than other parts, which mortality to female insects was more than 60 %. And the purity of 6th part has been above 90 % by HPLC. After detailed gradient detection, it was found that the extraction rate of 6th part from crude extracts was 6.34% in the concentration of 10mg·mL-1, and the female insect mortality was 98.45 %. On the other hand, the effect of 6th part on relative enzymes activity in Tetranychus cinnabarinus was also researched by colorimetric method. The results showed that the activity of AchE and Na+, K+-ATPase in Tetranychus cinnabarinus were 0.47 and 0.62 times as the control after the exposure in the concentration of 10 mg·mL-1 at 8 hours.

Keywords

Tetranychus cinnabarinus Juglans regia acetylcholine esterase Na+ K+-ATPase 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. 1.
    Cao, H., Wang, Y., Shi, G., et al.: Effects of the Chloroform Extracts of Kochia scoparia to Several Enzyme Systems in Tetranychus viennensis. Scientia Silvae Sinicae 43(2), 68–72 (2007) (in Chinese)Google Scholar
  2. 2.
    He, Y., Zhan, R., Zhao, Y.: Advances in Research and Application of Botanical Pesticides. Chinese Journal of Tropical Agriculture 24(3), 58–62 (2004) (in Chinese)Google Scholar
  3. 3.
    Lin, Z., Zhang, L.: The Effecy of Phophine on Acetylcholine esterase in Adults of Rhyzopertha Dominica(Fabricius) and Sitophilus Zeamais(Motschulsky) in Vivo. Journal of Zhengzhou Institute of Technology 22(4), 35–41 (2001) (in Chinese)Google Scholar
  4. 4.
    Li, L., Li, L., Fan, Y., et al.: Insect Pests of Chinese Jujube. China Agriculture Press, Beijing (1992) (in Chinese)Google Scholar
  5. 5.
    Shi, G., Zheng, W., Dang, Z., et al.: Insect Pests of Fruit Trees. China Agriculture Press, Beijing (1994) (in Chinese)Google Scholar
  6. 6.
    Tang, J.: Study of the Bioactive Constituents from the Leaves of Juglans Regia. Master Thesis in Northwest Agriculture&Forestry University (2003) (in Chinese)Google Scholar
  7. 7.
    Zhang, X.: Function and Niche of Botanical Pesticide. World Agriculture 2(1), 31–32 (2003) (in Chinese)Google Scholar
  8. 8.
    Zhai, M., Li, X., Lin, Q., et al.: Extract of Inhibiting Composition and Inhibitory Activity from Walnut Leaves. Journal of Northwest Forestry University 18(4), 89–91 (2003) (in Chinese)Google Scholar
  9. 9.
    Zhai, M., Yang, X., Lin, Q., et al.: A Study on the Bioactivity of Walnut Leaves Against Stilpnotia candida. Journal of Northwest Forestry University 18(2), 65–67 (2003) (in Chinese)Google Scholar
  10. 10.
    Zhang, Z.: New Developments of Insect Toxicology. Peking University Press, Beijing (1982) (in Chinese)Google Scholar
  11. 11.
    Zhai, M., Yang, X., Liu, L.: The Contact Effect of Extracts from Walnut Leaves on Aphid. Journal of Northwest Forestry University 16(4), 55–56 (2001) (in Chinese)Google Scholar
  12. 12.
    Zhu, L., Ni, Y., Huang, C., et al.: Multiple Bioassy Method to Tetranychus cinnabarinus. Journal of Nanjing Agricultural Technology College 17(1), 13–17 (2002)Google Scholar
  13. 13.
    Abbort, W.S.: A method of computing the effectiveness of an insecticide. J. Am. Mosq. Control Assoc. 3, 302–303 (1987)Google Scholar
  14. 14.
    FAO. Revised method for spider mites and their eggs (Tetranychus spp. and Panonychus ulmi Koch). FAO Plant Production 21, 49–54 (1980) Google Scholar
  15. 15.
    Hazan, A., Gerson, U., Tahori, A.S.: Spider mite webbing I. the production of webbing under various enviromental conditions. Acarologia 16, 68–84 (1974)Google Scholar
  16. 16.
    Ho, C., Maggi, V., et al.: Spider mite (Acari: Tetranychidae) on various crops in Taiwan. J. Agr. Res. China. 46, 333–346 (1997)Google Scholar
  17. 17.
    Mahoney, N., Molyneux, R.J., Mckenna, J., Leslie, C., Mcgranahan, G.: Resistance of ’Tulare’ Walnut (Juglans regia cv. Tulare) to aflatoxigenesis. J. Fo. Sci. 68(2), 619–621 (2003)CrossRefGoogle Scholar
  18. 18.
    Dekeyser, M.A., Downer, R.G.H.: Biochemical and Physiological Targets for Miticides. Pestic. Sci. 40, 85–101 (1994)CrossRefGoogle Scholar
  19. 19.
    Sachiko, K., Youko, K., Hamitsu, K.: A novel miticide. Norio O and Tadakazu WAKD-2023: Biological activity and mode of action IUPAC Congress (1999)Google Scholar
  20. 20.
    Wang, Y., Shi, G., Zhao, L., et al.: Acaridical activity of Juglans regia leaf extracts on Teranychus viennensis and Tetranychus cinnabarinus (Acari: Tetranychidae). J. Econ. Entomo. 100, 1298–1303 (2007)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag GmbH Berlin Heidelberg 2012

Authors and Affiliations

  • You-nian Wang
    • 1
  • Hai-xiang Wang
    • 1
  • Yu-bo Liu
    • 1
  • Guang-lu Shi
    • 1
  1. 1.Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. ChinaBeijing University of AgricultureBeijingChina

Personalised recommendations