Effects of Mentha Piperita Extracts on Activities of Several Enzymes of Tetranychus Cinnabarinus

  • Jian-ju Ren
  • Guang-lu Shi
  • Lan-qing Ma
  • Yu-bo Liu
  • You-nian Wang
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 134)


The specific acaricidal mechanisms of Mentha piperita extracts were studied. The acaricidal and relative enzyme activity in Tetranychus cinnabarinus after treatment with Mentha piperita extracts at the concentration of 2mg·ml- 1 was studied by using the slide dip technique and colorimetric method, respectively. The effects of Mentha piperita extracts on the poisonous symptom and ultrastructure of the treated mites were also observed. Our results showed that fraction V from Mentha piperita extracts had stronger toxicity than others. The mites had experienced four phases namely quiescence, excitement, spasm and death after treatment, indicating that the Mentha piperita extracts were able to cause the poisonous symptom of the treated mites. Enzyme activity assay showed that the activities of glutathione-S-transferase (GSTs) and monoamine oxidase (MAO) were strongly induced whereas the activities of acetylcholine esterase (AChE) and protease were restrained after treatment. The changes of these enzymes led to the death of mites due to the destruction in the nervous transmission and digestive system. The transmission electron microscopic observations revealed that the treated mites suffered from the rupture of ultrastructure such as endoplasmic reticulum, mitochondria and nuclear membrane. These data provided useful information for the use of Mentha piperita as a novel resource of botanical acaricide.


Mentha piperita Tetranychus cinnabarinus activity of enzyme ultrastructure 


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  1. 1.
    Antonious, G.F., Dahlman, D.L., Hawkins, L.M.: Insecticidal and Acaricidal Performance of Methyl Ketones in Wild Tomato Leaves Bull. Environ. Contam. Toxicol 71, 400–407 (2003)CrossRefGoogle Scholar
  2. 2.
    Irfan, A., Hikmet, O., Onder, C., Fikrettin, S.: Toxicity of essential oil vapours to two greenhouse pests, Tetranychus urticae Koch and Bemisia tabaci. Genn. Industrial Crops and Products 19, 167–173 (2004)CrossRefGoogle Scholar
  3. 3.
    Mansour, F., Ravid, U., Putievsky, E.: Studies on the effects of essential oils isolated from 14 species of Labiatae on the carmine spider mite, Tetranychus cinnabarinus. Phytoparasitica 14, 137–142 (1986)CrossRefGoogle Scholar
  4. 4.
    Shaaya, F., Ravid, U., Paster, N., Juven, B., Zisman, U., Pissarev, V.: Fumigant toxicity of essential oils against four major stored product insects. J. Chem. Ecol 17, 499–504 (1991)CrossRefGoogle Scholar
  5. 5.
    Ho, S.H., Ma, Y., Huang, Y.: Anethole apotential insecticide from Illicium verum Hook F against two stored-product insects. Int. Pest Control 39, 50–51 (1997)Google Scholar
  6. 6.
    Landolt, P.J., Hofstetter, R.W., Biddick, L.L.: Plant essential oils as arrestants and repellents for neonate larvae of the codling moth (Lepidoptera: Totricidae). Environ. Entomol. 28(6), 954–960 (1999)Google Scholar
  7. 7.
    Liu, J.H.: environmental-friendly botanical pesticide. Journal of Biology 37(6), 30–31 (2002)Google Scholar
  8. 8.
    Ardeshir, A., Rahim, E., Gholamhosein, T.: Laboratory evaluation of some plant essences to control Varroa destructor (Acari: Varroidae). Experimental and Applied Acarology 27, 319–327 (2002)CrossRefGoogle Scholar
  9. 9.
    Committee of National Codex. Codex of People’s Republic of China, Beijing: People’ sanitation publishing company, 310 (2000)Google Scholar
  10. 10.
    Liang, C.Y., Li, W.L., Zhang, H.Q., et al.: The advance on the research of chemical constituents and pharmacological activities of Mentha haplocalyx. Chinese Wild Plant Resources 22(3), 91 (2003)MathSciNetGoogle Scholar
  11. 11.
    Chen, G.L., Yu, Y.B., Li, D.M.: Primary study of the effective constitudes and pharmacologic on peppermint oil. Chinese Journal of Infermation on TCM 7(2), 33–34 (2000)Google Scholar
  12. 12.
    Edris, A.E., Farrag, E.S.: Antifungal activity of peppermint an sweet basil essential oils and their majority constituents of some plant pathogenic fungi from the vaporphase. Nahrung 47(2), 117–121 (2003)CrossRefGoogle Scholar
  13. 13.
    Schuhmacher, A., Reichling, J., Schnitzler, P.: Virucidal effect of peppermint oil on the developed viruses herpess implex virus type and type in vitro. Phytomedicine 10(6-7), 504–510 (2003)CrossRefGoogle Scholar
  14. 14.
    Ahmed, S.M., Eapen, M.: Vapour toxicity and repellency of some essential oils to insect pests. Indian Perfumer 30(1), 273–278 (1986)Google Scholar
  15. 15.
    Raja, N., Albert, S., Ignacimuthu, S.: Effect of plant volatile oils in protecting stored cowpea Vigna unguiculata (L.) Walpers against Callosobruchus maculates (F.) (Coleoptera: Bruchidae) infestation. Journal of Stored Products Research 37, 127–132 (2001)CrossRefGoogle Scholar
  16. 16.
    Jeremy, A., Rudd: Effects of pesticides on spin down and webbing production by the two-spotted spider mite, Tetranychus urticae Koch (Acari: Tetranychidae). Experimental & Applied Acarology 21, 615–628 (1997)CrossRefGoogle Scholar
  17. 17.
    Cao, H., Liu, S.Q., Wang, H.L., et al.: The effects of extracts of Tateges eracta on activities of several enzyme of Tetranychus viennensis zacher. Scientia Silvae Sinicae 39(2), 114–115 (2003)MathSciNetGoogle Scholar
  18. 18.
    Mu, L.Y.: Methodology of plant chemical protection, pp. 160–161. China Agriculture publishing company, Beijing (1994)Google Scholar
  19. 19.
    Qu, J.H., Chen, J.C., Hu, G.D., et al.: Dynamic changes of catalase and glutathione-s-transferase in the different tissues of tilapia exposed to phenol. Ecology and Environment 15(4), 687–692 (2006)Google Scholar
  20. 20.
    Chen, F.J., Gao, X.W., Lei, M.Q., et al.: Effects of tannic acid on glutathione S-transferases in Helicoverpa armigera(Hǖbner). Acta Entomologica Sinica 46(6), 684–690 (2003)Google Scholar
  21. 21.
    Ku, C.C., Chiang, F.M., Hsin, C.Y., YaoY, E., Sun, C.N.: Glutathion transferases isozymes involved in insecticide resistance of diamond bacmoth larvae. Pestic. Biochem. Physiol 50, 191–197 (1994)CrossRefGoogle Scholar
  22. 22.
    Prapanthadara, L., Promter, N., Koottathep, S., Somboon, P., Ketterman, A.J.: Isoenzymes of glutathione-S-transferasess from the mosquito Anopheles dirus species B: the purification,partial characterization and interaction with various insecticides. Insect Biochem. Mol. Biol 30, 395–403 (2000)CrossRefGoogle Scholar
  23. 23.
    Wang, D., Tang, Z.H., Shang, J.Y., et al.: Advances in the studies of acetylcholinesterase genes from insects. Acta Entomologica Sinica 49(3), 497–503 (2006) (in Chinese)Google Scholar
  24. 24.
    Chen, J.C., Wu, W., Qu, J.H., et al.: Activities of catalase and monoamine oxidase in different tissues of tilipia under stress of deltamethrin. Journal of Agro-Environment Science 25(6), 1441–1445 (2006)Google Scholar
  25. 25.
    Cao, H., Wang, Y.N., Liu, S.Q., et al.: Effects of the Chloroform Extracts of Kochia scoparia to Several Enzyme Systems in Tetranychus viennensis. Scientia Silvae Sinicae 42(2), 68–72 (2007)Google Scholar
  26. 26.
    Wang, Y.N., Shi, G.L., Zhao, L.L., et al.: Acaricidal activity of Juglans regia leaf extracts on Tetranychus viennensis and Tetranychus cinnabarinus (Acari: Teranychidae). Journal of Economic Entomology 100(4), 1298–1303 (2007)CrossRefGoogle Scholar
  27. 27.
    Abbott, W.S.: A method for computing the effectiveness of an insecticide. Econ. Entomol. 18, 265–267 (1925)Google Scholar
  28. 28.
    FAO. Revised method for spidermites and their eggs(Tetranychus spp.and Panonychus ulmi Koch). FAO Plant Production 21, 49–54 (1980)Google Scholar

Copyright information

© Springer-Verlag GmbH Berlin Heidelberg 2012

Authors and Affiliations

  • Jian-ju Ren
    • 1
  • Guang-lu Shi
    • 1
  • Lan-qing Ma
    • 1
  • Yu-bo Liu
    • 1
  • You-nian Wang
    • 1
  1. 1.Key Laboratory of Urban Agriculture (North) of Ministry of Agriculture, P.R. ChinaBeijing University of AgricultureBeijingChina

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