The Effects of Lupeol from the Petroleum Ether Extract of Inula Britanica on the Biological and Enzyme Activity of Tetranychus Cinnabarinus
The acaricidal and relative enzyme activity in Tetranychus cinnabarinus treated with petroleum ether extract of Inula britanica were studied. Our results indicated that petroleum ether extract of Inula britanica had acaricidal activities against Tetranychus cinnabarinus, and its mortality to Tetranychus cinnabarinus had reached 92.05 % at the concentration of 2mg∙mL-1. After liquid chromatography and thin layer chromatography, there were 38 fractions gained in which fraction 7 had stronger toxicity than others, and its mortality to Tetranychus cinnabarinus was 92.05 %. Fraction 7 was identified as Lupeol by CG/MS, and the mortality of Lupeol to Tetranychus cinnabarinus was 66.46%. The activities of glutathione-S-transferase, Ca2+ - ATPase, SOD and protein content were assayed by colorimetric method after treatment with Lupeol. Our results showed that the activity of SOD was strongly induced whereas the activities of glutathione-S-transferase and Ca2+- ATPase were restrained, but there was little change in total protein content. These results suggested Lupeol from the petroleum ether extract of Inula britanica had acaricidal activities against Tetranychus cinnabarinus. These data provided useful information for the use of Inula britanica as a novel resource of botanical acaricide.
KeywordsInula britanica Petroleum Tetranychus cinnabarinus enzyme activity
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- 1.Abbott, W.S.: A method of computing the effectiveness of an insecticide. J. Econ. Entomol. 3, 302–303 (1987)Google Scholar
- 2.Cao, H., Liu, S.Q., Shi, G.L., et al.: The effects of the extracts from stellera chamaejasmel on the biological and enzyme activity of tetranychus viennensis zacher. Science Silvae Sinicae 39(1), 98–102 (1957)Google Scholar
- 3.Dalale, N., Baudouin, C., et al.: Lupeol stimulates the production of high-quality type I collagen in human skin through HSP47 induction. Journal of the American Academy of Dermatology 58(2), AB62 (2008)Google Scholar
- 4.Food and Agriculture Organization. Revised method for spider mites and their eggs (Tetranychus spp. and Panonychus ulmi Koch). FAO Plant Prod. Prot. 21, 49–54 (1980)Google Scholar
- 6.Guo, Q.L., Yang, J.S., et al.: Sesquiterpenes in Inula L. Plants and Their Pharmacological Activities 17(6), 804–809 (2005)Google Scholar
- 7.Li, J., Zhang, Y.T., Zheng, W., et al.: Commasie brilliant blue staining was used to study the total protein content. Chinese Journal of Biologicals 13(2), 118–120 (2000)Google Scholar
- 8.Liu, S.Y., Sun, L.G., Xing, W., et al.: The effect of chronic lead exposure on hippocampal intraneuronal [Ca2 + ] and activity of Ca2 + -ATPase in rats. Journal of Health Toxicology 13(1), 102–103 (1999)Google Scholar
- 9.Saleem, M.: Lupeol, a novel anti-inflammatory and anti-cancer dietary triterpene. Cancer Lett. 2009 (2000) (Epub ahead of print)Google Scholar
- 10.Shen, Z.J., Wang, H.X., Shi, G.L., et al.: Biological Activities of Extracts from 3 Species of Plants Against Tetranychus cinnabarinus. Journal of Beijing Agricultural College 23(1), 22–24 (2008)Google Scholar
- 14.Wang, Y., Shi, G.L., Wang, Y.N., et al.: Acaricidic Activity of Herbal Extracts Against Tetranychus cinnabarinus 24(5), 323–326 (2008)Google Scholar
- 16.Zhao, L.L., Liu, S.Q., Cao, H., et al.: Primary Studies of Acarocidal Activity of Wikstroemia chamedaphne against Tetranychus viennensis. Scientia Silvae Sinicae 41(6), 65–70 (2005)Google Scholar