Detection Class I Integron of Multidrug Resistace Pathogenic Salmonella from Chicken

  • Qiuyue Wang
  • Yanying Zhang
  • Qiumei Shi
  • Wei Yao
  • Cuizhen Chen
  • Guangping Gao
  • Donglin Zhang
  • Hai Fang
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 134)

Abstract

Antimicrobial resistance test of 59 Samonella isolated from chicken was done by K-B method. The drug-resistance rate of oxacillin, tetracycline, erythromycin, penicillin G, gentamicin was 74.6%, 64.4%, 52.5%, 47.5% and 37.3% respectively. Most of the isolates were multidrug resistance and the rate was 81.36% (48/59). Among the total of the multidrug resistance isolates, the rate of resistance to three, four and five drugs was 45.76% (27/59), 23.73% (14/59) and 11.86% (7/59). PCR and DNA sequencing were used for screening and characterization of class I integrons. As a result, the detection rate of class I integron was 40.68% (24/59), and among these 48 isolates which had been proven multidrug-resistant, the detection rate of class I integron was 50% (24/48). Class I integron PCR positive isolates rate of multidrug-resistance was 100% (24/24). Results show that the class I integron has the relationship with multidrug resistance.

Keywords

Samonella class I integron multidrug resistance detection 

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Copyright information

© Springer-Verlag GmbH Berlin Heidelberg 2012

Authors and Affiliations

  • Qiuyue Wang
    • 1
  • Yanying Zhang
    • 1
  • Qiumei Shi
    • 1
  • Wei Yao
    • 2
  • Cuizhen Chen
    • 1
  • Guangping Gao
    • 1
  • Donglin Zhang
    • 1
  • Hai Fang
    • 1
  1. 1.Key Laboratory of Preventive Veterinary Medicine of Hebei ProvinceHebei Normal University of Science and TechnologyQinhuangdaoChina
  2. 2.Heibei Qinglong County Pingfangzi Junior Middle SchoolQinhuangdaoChina

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