Abstract
Antimicrobial resistance test of 59 Samonella isolated from chicken was done by K-B method. The drug-resistance rate of oxacillin, tetracycline, erythromycin, penicillin G, gentamicin was 74.6%, 64.4%, 52.5%, 47.5% and 37.3% respectively. Most of the isolates were multidrug resistance and the rate was 81.36% (48/59). Among the total of the multidrug resistance isolates, the rate of resistance to three, four and five drugs was 45.76% (27/59), 23.73% (14/59) and 11.86% (7/59). PCR and DNA sequencing were used for screening and characterization of class I integrons. As a result, the detection rate of class I integron was 40.68% (24/59), and among these 48 isolates which had been proven multidrug-resistant, the detection rate of class I integron was 50% (24/48). Class I integron PCR positive isolates rate of multidrug-resistance was 100% (24/24). Results show that the class I integron has the relationship with multidrug resistance.
This work is partially supported by Hebei Science and Technology Support Program (09220402D).
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References
Hall, A.J., Saito, E.K.: Avian wildlife mortality events due to salmonellosis in the united states,1985–2004. Journal of Wildlife Diseases 44(3), 585–593 (2008)
Maguire, A.J., Brown, D.F.J., Gray, J.J., Desselberger, U.: Rapid screening technique for class I integrons in Enterobacteriaceae and nonfermenting gram-negative bacteria and its use in molecular epidemiology. Antimicrob. Agents Chemother. 45, 1022–1029 (2001)
Vo, A.T.T., van Duijkeren, E., Fluit, A.C., Wannet, W.J.B., Verbruggen, A.J., Maas, H.M.E., Gaastra, W.: Antibiotic resistance, integrons and Salmonella genomic island 1 among non-typhoidal Salmonella serovars in The Netherlands. Int. J. Antimicrob. 28, 172–179 (2006)
Guerra, B., Soto, S., Cal, S., Mendoza, M.C.: Antimicrobial resistance and spread of class I integrons among Salmonella serotypes. Antimicrob. Agents Chemother. 44, 2166–2169 (2000)
Nogrady, N., Gado, I., Toth, A., Paszti, J.: Antibiotic resistance and class I integron patterns of non-typhoidal human Salmonella serotypes isolated in Hungary in 2002 and 2003. Int. J. Antimicrob. 26, 126–132 (2005)
Gebreyes, W.A., Thakur, S., Davies, P.R., Funk, J.A., Altier, C.: Trends in antimicrobial resistance, phage types and integrons among Salmonella serotypes from pigs, 1997–2000. J. Antimicrob. Chemother. 53, 997–1003 (2004)
Rao, S., Maddox, C.W., Hoien-Dalen, P.: Diagnostic Accuracy of Class I Integron PCR Method in Detection of Antibiotic Resistance in Salmonella Isolates from Swine Production Systems. Journal of Clinical Microbiology 46(3), 916–920 (2008)
Chiu, C.H., Ou, J.T.: Rapid identification of Salmonella serovars in feces by specific detection of virulence genes invA and spvC by an enrichment broth culture-multiplex PCR combination assay. J. Clin. Microbiol. 34, 2619–2622 (1996)
Wayne, P.A.: NCCLS. Performance standards for antimicrobial susceptibility testing. Eleventh International Supplement. Veterinary Antimicrobial Susceptibility Testing Document M31-A2, 21(1) (2001)
Yang, C.-M., Lin, M.-F., Lin, C.-H.: Characterization of antimicrobial resistance patterns and integrons in human fecal Escherichia coli in Taiwan. Jpn. J. Infect. Dis. 62, 177–181 (2009)
Hall, R.M., Collis, C.M.: Mobile gene cassettes and integrons: capture and spread of genes by site-specific recombination. Mol. Microbiol. 15, 593–600 (1995)
Rao, S., Maddox, C.W., Hoien-Dalen, P., Lanka, S., Weigel, R.M.: Diagnostic Accuracy of Class I Integron PCR Method in Detection of Antibiotic Resistance in Salmonella Isolates from Swine Production Systems. Journal of Clinical Microbiology 3, 916–920 (2008)
Daly, M., Fanning, S.: Characterization and chromosomal mapping of antimicrobial resistance genes in Salmonella enterica serotype Typhimurium. Appl. Environ. Microbiol. 66, 4842–4848 (2000)
Guerra, B., Soto, S., Cal, S., Mendoza, M.C.: Antimicrobial resistance and spread of class I integrons among Salmonella serotypes. Antimicrob. Agents Chemother. 44, 2166–2169 (2000)
Guerra, B., Soto, S.M., Arguelles, J.M., Carmen Mendoza, M.: Multidrug Resistance Is Mediated by Large Plasmids Carrying a Class I Integron in the Emergent Salmonella enterica Serotype [4,5,12:i:2]. Antimicrobial Agents and Chemotherapy 45(4), 1305–1308 (2001)
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Wang, Q. et al. (2012). Detection Class I Integron of Multidrug Resistace Pathogenic Salmonella from Chicken. In: Zhu, E., Sambath, S. (eds) Information Technology and Agricultural Engineering. Advances in Intelligent and Soft Computing, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27537-1_61
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DOI: https://doi.org/10.1007/978-3-642-27537-1_61
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