Abstract
In order to deeply understand the effect of Salinization Resistance Transgenic Soybean (SRTS) on diversity of rhizospheric bacteria in saline soils in Songnen Plain, Heilongjiang Province, DGGE- cloning and traditional culture-depending methods were used. DGGE fingerprint analysis results indicated that the bacterial diversity index and evenness index in rhizospheric soil of SRTS were lower than other soybean’s based on analysis to the number of bands of bacterial 16SrDNA. DGGE-cloning sequencing results showed that SRTS has a certain inhibition effect on rhizospheric soil bacteria phylum Firmicutes and decreased the priority of phylum Proteobacteria, yet had no effect on rhizosphere soil bacteria phylum Acidobacteria. The study revealed that the release of SRTS in saline soil decreased the amount and the diversity of bacteria in rhizospheric soil to some extent, and phylum Alphaproteobacteria genus Sphingomonas was promoted in rhizospheric soil.
This work is partially supported by Genetically Modified Breeding Projects 2009ZX08011- 025B-02 to Honhyan Wang; NEAU Scientific Foundation and NEAU Innovation Team Foundation (CXT003-3-2) to Zhihua Liu.
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Wang, Z., Liu, Z., Wang, H. (2012). Salinization Resistance Transgenic Soybean Reduced Bacterial Diversity in Rhizosphere. In: Zhu, E., Sambath, S. (eds) Information Technology and Agricultural Engineering. Advances in Intelligent and Soft Computing, vol 134. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27537-1_47
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DOI: https://doi.org/10.1007/978-3-642-27537-1_47
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