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3D Observation and Preliminary Quantitative Analysis of Germinal Vesicle Oocytes in Aging and Pubertal Mice

  • Ning Tian
  • Lu Zhang
  • Jing-gao Zheng
  • Die-yan Chen
  • Ying Li
  • Wan-yun Ma
Part of the Advances in Intelligent and Soft Computing book series (AINSC, volume 134)

Abstract

The method involved in this paper may bring new prospects for research of immature oocytes quality between the aging mice and the pubertal mice. By adopting the two-photon excitation laser scanning microscopy and studying nucleolus morphological parameter such as the shape, volume size and location, more abundant information were obtained about the immature oocytes of aging and pubertal mice, and may provide a broader approach to search the relationship between the oocyte quality and maternal-age effect as well as other predisposing factors.

Keywords

Aging mouse Nucleolus Germinal vesicle oocytes 

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Copyright information

© Springer-Verlag GmbH Berlin Heidelberg 2012

Authors and Affiliations

  • Ning Tian
    • 1
  • Lu Zhang
    • 2
  • Jing-gao Zheng
    • 1
  • Die-yan Chen
    • 1
  • Ying Li
    • 2
  • Wan-yun Ma
    • 1
  1. 1.Key Laboratory for Atomic and Molecular Nanosciences of Education Ministry, Department of PhysicsTsinghua UniversityBeijingPeople’s Republic of China
  2. 2.Department of Histology and Embryology, School of Basic Medical SciencesPeking University Health Science CenterBeijingPeople’s Republic of China

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