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From Nucleic Acids Sequences to Molecular Medicine pp 429–452Cite as

Suppression of Hepatitis C Viral Genome Replication with RNA-Cleaving Deoxyribozyme

Part of the RNA Technologies book series (RNATECHN)

Abstract

Downregulation of viral genes via oligonucleotide-based gene therapy is a potential strategy for the treatment of virus infection such as hepatitis C. Hepatitis C virus (HCV) is a small-sized, enveloped, positive-sense single-stranded RNA virus. As HCV has highly mutative properties and strong drug resistance, effective antiviral drug for HCV infection is currently unavailable. One of the potential therapeutic strategies for hepatitis C treatment is to cleave HCV RNA genome with proper antisense nucleic acids, thereby inhibiting virus replication in host. RNA-cleaving antisense oligodeoxyribozyme, known as DNAzyme, is an attractive therapeutic oligonucleotide which enables cleavage of mRNA in a sequence-specific manner and thus silencing target gene. In this chapter, we discuss current status of functional antisense oligonucleotides that have been applied to inhibit HCV replication in vitro and in vivo. In particular, the DNAzyme and the DNAzyme conjugated nanoparticle system are discussed in detail to demonstrate a successful usage of functional oligonucleotide and its delivery in vivo for further therapeutic application of functional oligonucleotides in the treatment of hepatitis C.

Keywords

  • Antisense oligonucleotide
  • DNAzyme
  • Hepatitis C virus
  • Nanomaterial-based gene delivery
  • RNA cleavage

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Acknowledgments

This work was supported by NRF grants (2010-0019306, 2011-0016385) and the WCU project (R33-10128) funded by the MEST, Republic of Korea.

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Authors and Affiliations

  1. Department of Chemistry, Seoul National University, Seoul, 151-747, Republic of Korea

    Dal-Hee Min

  2. WCU Program and Basic Research Laboratory, Department of Bioscience and Biotechnology, Konkuk University, Seoul, 143-701, Republic of Korea

    Dong-Eun Kim

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  1. Institute of Chemistry/Biochemistry, Free University Berlin, Thielallee 63, Berlin, 14195, Berlin, Germany

    Volker A. Erdmann

  2. Inst. Bioorganic Chemistry, PAN Poznan, ul. Z. Noskowskiego 12/14, Poznan, 61-704, Poland

    Jan Barciszewski

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Min, DH., Kim, DE. (2012). Suppression of Hepatitis C Viral Genome Replication with RNA-Cleaving Deoxyribozyme. In: Erdmann, V., Barciszewski, J. (eds) From Nucleic Acids Sequences to Molecular Medicine. RNA Technologies. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-27426-8_17

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